Improved Recovery of Erythropoietin and Darbepoetin from Equine Plasma by the Application of a Wheat Germ Agglutinin Mediated Pre-Extraction Prior to Immunoaffinity Chromatography

Stanley, Shawn M.R.; Chua, Danny

doi:10.4236/abb.2014.57077

Cited by 3 publications

(1 citation statement)

References 29 publications

(33 reference statements)

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“…Extraction of two recombinant human glycoproteins-erythropoietin and Darbepoetinfrom equine plasma by immunoaffinity chromatography has been shown to be improved by 15% when pretreating the samples with wheat germ agglutinin immobilized on Sepharose gel [74]. The technique is easily scalable and indicates the WGA potential for naturally or synthetically glycosylated recombinant protein industry.…”

Section: Non-medical Wga Applicationsmentioning

confidence: 99%

Wheat Germ Agglutinin—From Toxicity to Biomedical Applications

Balčiūnaitė-Murzienė

Dzikaras

2021

Applied Sciences

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Wheat germ agglutinin is a hevein class N-Acetylglucosamine–binding protein with specific toxicity and biomedical potential. It is extractable from wheat germ—a low-value byproduct of the wheat industry—using well–established extraction methods based on salt precipitation and affinity chromatography. Due to its N-Acetylglucosamine affinity, wheat germ agglutinin exhibits antifungal properties as well as cytotoxic properties. Its anticancer properties have been demonstrated for various cancer cells, and toxicity mechanisms are well described. Wheat germ agglutinin has been demonstrated as a viable solution for various biomedical and therapeutic applications, such as chemotherapy, targeted drug delivery, antibiotic-resistant bacteria monitoring and elimination. This is performed mostly in conjunction with nanoparticles, liposomes, and other carrier mechanisms via surface functionalization. Combined with abundant wheat byproduct sources, wheat germ agglutinin has the potential to improve the biomedical field considerably.

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Section: Non-medical Wga Applicationsmentioning

confidence: 99%

Wheat Germ Agglutinin—From Toxicity to Biomedical Applications

Balčiūnaitė-Murzienė

Dzikaras

2021

Applied Sciences

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Fabrication of an on-line enzyme micro-reactor coupled to liquid chromatography–tandem mass spectrometry for the digestion of recombinant human erythropoietin

Foo

Smith

Stanley³

2015

Talanta

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Wheat Germ Agglutinin (WGA): Its Nature, Biological Role, Significance in Human Nutrition, and Possibility to Be Used as Marker of Whole-Grain Status in Wheat-Based Foods

Carcea,

Melloni,

Narducci

et al. 2024

Foods

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The growing scientific evidence on the health benefits of whole-grain food consumption has promoted the manufacturing of a great number of products differing in quality and content of whole-grain components. This is particularly true for commercial wheat-based products where it is not always clear how much whole wheat is present considering that in many cases, they are manufactured from reconstituted mill streams and that there is not a standardised globally accepted definition and metrics to objectively evaluate whole-grain status. Attempts have been made to assess the level of “wholegraininess” in wheat products by measuring specific constituents that correlate with different wheat tissues, especially those that are expected to be found in a true whole-grain wheat product. Wheat germ agglutinin (WGA), a small lectin protein present exclusively in the wheat-germ tissues, has been indicated by several scientists as one of these constituents and after founding that its level changes depending on the amount of germ found in a wheat flour, it has been indicated as a biomarker of whole-grain status for wheat products. In this review, the biochemistry of WGA, its methods of detection, and current knowledge on its possibility to be practically utilized as a reliable marker are critically discussed.

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Improved Recovery of Erythropoietin and Darbepoetin from Equine Plasma by the Application of a Wheat Germ Agglutinin Mediated Pre-Extraction Prior to Immunoaffinity Chromatography

Cited by 3 publications

References 29 publications

Wheat Germ Agglutinin—From Toxicity to Biomedical Applications

Wheat Germ Agglutinin—From Toxicity to Biomedical Applications

Fabrication of an on-line enzyme micro-reactor coupled to liquid chromatography–tandem mass spectrometry for the digestion of recombinant human erythropoietin

Wheat Germ Agglutinin (WGA): Its Nature, Biological Role, Significance in Human Nutrition, and Possibility to Be Used as Marker of Whole-Grain Status in Wheat-Based Foods

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