Improved quantitative detection of VBNC Vibrio parahaemolyticus using immunomagnetic separation and PMAxx-qPCR

Zhao, Linna; Lv, Xinrui; Xiao, Cao; Zhang, Jingfeng; Gu, Xiaojing; Zeng, Haiyan; Wang, Li

doi:10.1016/j.foodcont.2019.106962

Cited by 27 publications

(15 citation statements)

References 28 publications

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“…They basically work through a perfect synergy between the selective biological or biomimetic recognition element and the transducer (e.g., optical, electrical, electrochemical, etc.) operating the target revelation [ 153 , 154 ]. If opportunely implemented, biosensors can provide a fast revelation of bacteria together with a high degree of miniaturization and integration of the entire analytical system, ideal for in situ and remote-controlled applications.…”

Section: Viable But Non Culturable (Vbnc) Statementioning

confidence: 99%

Environmental Management of Legionella in Domestic Water Systems: Consolidated and Innovative Approaches for Disinfection Methods and Risk Assessment

et al. 2021

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Legionella is able to remain in water as free-living planktonic bacteria or to grow within biofilms that adhere to the pipes. It is also able to enter amoebas or to switch into a viable but not culturable (VBNC) state, which contributes to its resistance to harsh conditions and hinders its detection in water. Factors regulating Legionella growth, such as environmental conditions, type and concentration of available organic and inorganic nutrients, presence of protozoa, spatial location of microorganisms, metal plumbing components, and associated corrosion products are important for Legionella survival and growth. Finally, water treatment and distribution conditions may affect each of these factors. A deeper comprehension of Legionella interactions in water distribution systems with the environmental conditions is needed for better control of the colonization. To this purpose, the implementation of water management plans is the main prevention measure against Legionella. A water management program requires coordination among building managers, health care providers, and Public Health professionals. The review reports a comprehensive view of the state of the art and the promising perspectives of both monitoring and disinfection methods against Legionella in water, focusing on the main current challenges concerning the Public Health sector.

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Section: Viable But Non Culturable (Vbnc) Statementioning

confidence: 99%

Environmental Management of Legionella in Domestic Water Systems: Consolidated and Innovative Approaches for Disinfection Methods and Risk Assessment

et al. 2021

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“…Recently, Zhao et al . developed an immunomagnetic separation‐improved propidium monoazide‐qPCR assay and detected V. parahaemolyticus in raw shrimp to a level as low as 1.85 CFU g −1 in 4 h (Zhao et al ., 2020), which is similar to our RF‐SRCA method. However, the PCR‐based assays required tedious electrophoresis and costly thermocycle instruments.…”

Section: Resultsmentioning

confidence: 66%

“…The detection limits of multiplex droplet digital PCR (15 CFU mL −1 ) and a multiplex PCR assay (10 CFU g −1 ) were comparable to that of SRCA by electrophoresis, but 10-fold higher than that of RF-SRCA (Kim & Lee, 2014;Lei et al, 2020). Recently, Zhao et al developed an immunomagnetic separation-improved propidium monoazide-qPCR assay and detected V. parahaemolyticus in raw shrimp to a level as low as 1.85 CFU g −1 in 4 h (Zhao et al, 2020), which is similar to our RF-SRCA method. However, the PCR-based assays required tedious electrophoresis and costly thermocycle instruments.…”

Section: Detection Of V Parahaemolyticus In Artificially Contaminated Raw Oystermentioning

confidence: 98%

Development of real‐time fluorescence saltatory rolling circle amplification for rapid detection of Vibrio parahaemolyticus in seafood

Yuan

Yang

Zhang

et al. 2021

Int J of Food Sci Tech

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As a marine food-borne pathogen, Vibrio parahaemolyticus (V. parahaemolyticus) can cause human gastrointestinal disorders and pose a serious threat to food safety and public health worldwide. Using a portable scanner, herein, a real-time fluorescence saltatory rolling circle amplification (RF-SRCA) has been established for the rapid detection of V. parahaemolyticus in seafood targeting toxR gene. The RF-SRCA results could be effectively determined within 20-60 min via real-time fluorescence curve. Significant specificity of RF-SRCA was exhibited against 12 V. parahaemolyticus strains and 29 non-V. parahaemolyticus strains. The sensitivity and detection limit of V. parahaemolyticus in artificially contaminated raw oyster by RF-SRCA were 3.2 × 10 0 fg μL −1 and 2.3 × 10 0 CFU g −1 , respectively. Compared with SRCA by electrophoresis, RF-SRCA has higher sensitivity, lower detection limit, and avoids complicated electrophoresis. Compared with visual SRCA, the RF-SRCA takes shorter time, provides more accurate results and can eliminate the risk of cross-contamination. Moreover, 236 seafood samples were investigated for V. parahaemolyticus contamination, and the results showed 100% sensitivity, 95.88% specificity and 98.31% accuracy compared with the ISO method. Therefore, RF-SRCA possesses great potential as an accurate, specific, sensitive, rapid and convenient molecular diagnostic method for V. parahaemolyticus detection from various seafoods.

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“…The binding ratio of antibodies to MPs is one of the most significant parameters that determine the performance of IMS. Zhao et al (2020) investigated the influence of the antibody amounts (7.5, 15, 22.5, 30, and 60 µg per 0.5 mg MPs) on the enrichment of VBNC V. parahaemolyticus. As the antibody amount increased from 7.5 to 60 µg, the CE gradually increased and reached a maximum of 91.8% at 30 µg.…”

Section: The Molar Ratio Of Antibody To Mpsmentioning

confidence: 99%

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

Wang

Cai

Gao

et al. 2020

Comp Rev Food Sci Food Safe

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The high efficiency and accurate detection of foodborne pathogens and spoilage microorganisms in food are a task of great social, economic, and public health importance. However, the contamination levels of target bacteria in food samples are very low. Owing to the background interference of food ingredients and negative impact of nontarget flora, the establishment of efficient pretreatment techniques is very crucial for the detection of food microorganisms. With the sig

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Improved quantitative detection of VBNC Vibrio parahaemolyticus using immunomagnetic separation and PMAxx-qPCR

Cited by 27 publications

References 28 publications

Environmental Management of Legionella in Domestic Water Systems: Consolidated and Innovative Approaches for Disinfection Methods and Risk Assessment

Environmental Management of Legionella in Domestic Water Systems: Consolidated and Innovative Approaches for Disinfection Methods and Risk Assessment

Development of real‐time fluorescence saltatory rolling circle amplification for rapid detection of Vibrio parahaemolyticus in seafood

Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection

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