Improved propagation of vanilla (Vanilla planifolia Jacks. ex Andrews) using a temporary immersion system

Ramos-Castellá, Alma Laura; Iglesias-Andreu, Lourdes Georgina; Bello-Bello, Jericó Jabín; Lee-Espinosa, H. E.

doi:10.1007/s11627-014-9602-8

Cited by 60 publications

(30 citation statements)

References 23 publications

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“…Under this condition, the highest proliferation rate (23.5) was achieved. This result was consistent with that of Ramos-Castella et al [14], who obtained a maximum of 14.27 ± 0.89 for Vanilla planifolia shoots per explants with 2 min immersions every 4 h for the micropropagation by a TIBS. Gatica-Arias and Weber [15] reported the similar results for organogenic calli that high immersion frequency (every 6 h) induced shoot and promoted hop micropropagation.…”

Section: Discussionsupporting

confidence: 92%

“…In this study, it was indicated that longer immersion times induce vitrification, and also found that lower immersion frequency may prevent vitrification, but result in the production of vigorous plants. This results were consistent with the Ramos-Castella study on Vanilla plansifolia Jacks ex Andrews [14]. On the other hand, Albarran et al [19] reported that no relation between hyperhudricity of Coffea arabica somatic embryos in different immersion intervals.…”

Section: High Efficiency Of Propagation For Sagittaria Sagittifolia Usupporting

confidence: 87%

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High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

Gao¹,

Lin²,

Luo³

et al. 2016

JAST-A

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Abstract:Although several studies have reported on the propagation of the Sagittaria sagittifolia to date, none of these methods have efficiently achieved the mass production of these plants. The present study aimed to investigate the propagation and growth of S. sagittifolia using a temporary immersion bioreactor system (TIBS) compared with conventional semi-solid and liquid culture. The effect of different immersion frequencies and immersion times together with supplementation of various plant growth regulators to the Murashige and Skoog (MS) medium was evaluated on shoot proliferation and plant growth. The results showed that the higher immersion frequency (every 6 h) and shorter immersion time (3 min and 10 min) in medium containing 4 mg/L BA and 0.1 mg/L NAA produced the highest multiplication rate (23), which are significantly higher than conventional semi-solid (3.6) and liquid (4.5) method, and the best plant growth parameter. While, the lower immersion frequency and longer immersion time (30 min every 12 h and 60 min every 24 h) induced vitrification and pollution rate in shoot tips 16.6% and 19%, 42% and 37%, respectively. There is distinct decrease in pollution rate (8.3%) in TIBS (10 min every 6 h) compared with the conventional semi-solid and liquid cultures. Medium containing 4 mg/L BA and 0.5 mg/L NAA using 10 min immersion every 6 h showed satisfaction at the rooting stage, with high shoot proliferation rate (21.6), 100% rooting and 94% plant survival. Therefore, applying TIBS in S. sagittifolia is an efficient method for scaling up the production of plantlets with high quality seedlings.

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Section: Discussionsupporting

confidence: 92%

Section: High Efficiency Of Propagation For Sagittaria Sagittifolia Usupporting

confidence: 87%

High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

Gao¹,

Lin²,

Luo³

et al. 2016

JAST-A

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“…Furthermore, TIS showed better shoot and shoot bud proliferation as well as root and leaf induction numbers compared to SSS and CIS. Relatively similar results were found in Vanilla planifolia [25] and Rubus spp. as well [26].…”

Section: Discussionsupporting

confidence: 85%

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Kunakhonnuruk

Inthima

Kongbangkerd

2019

Biology

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Epipactis flava Seidenf. is an endangered Thai rheophytic orchid that has recently shown a rapid decrease in its natural habitat, prompting an urgent need for conservation using ex situ reintroduction methods. Temporary immersion system (TIS) has been successfully applied for large-scale propagation in various plants species. Propagation efficiency of E. flava using TIS was investigated and compared with conventional semi-solid system (SSS) and liquid continuous immersion system (CIS). The highest percentage of new shoot and shoot bud formation was obtained from TIS, followed by CIS and SSS, respectively. Growth parameters as indicated by number of new shoots, shoot buds, shoot height and leaves per explant were significantly higher using TIS than with SSS and CIS. Moreover, the maximum number of new shoots and shoot buds per replication were reliably obtained from TIS higher than SSS and CIS. After acclimatization, the highest survival percentage of plantlets was observed in TIS (76.7%), with 60% surviving after eight weeks of transplantation in artificial stream. TIS was determined as the most suitable culture system for in vitro mass propagation of E. flava compared to CIS and SSS.

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“…Many CIB plantlets were did not develop normally, and the results of in vitro conditions did not support the production of healthy plants. For this reason, many researchers have used the TIB system to increase the multiplication ratio and root initiation from in vitro plantlets (Yan et al 2011;Niemenak et al 2013;Akdemir et al 2014;Ramos-Castella et al 2014;Gao et al 2015). Relatively high humidity seemed to result in high stomatal density in the semi-solid medium.…”

Section: Discussionmentioning

confidence: 99%

Effects ofin vitroculture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipiferaL.) from somatic embryos

An¹,

Kim²,

Moon³

et al. 2016

J Plant Biotechnol

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We compared germination efficiency for somatic embryos (SE) of Liriodendron tulipifera using semi-solid (SS), temporary immersion bioreactors (TIB), and continuous immersion bioreactors (CIB) to produce vigorous plants. The bioreactors were designed to be immersed in liquid media with plantlets with an adjustable immersion time. TIB and CIB improved germination rates up to 80.86% and 95.21%, respectively, however, CIB produced more hyperhydric plantlets than TIB. The height of plantlets in TIB was significantly higher than for those in CIB. Fresh weights of plantlets grown in CIB of were significantly lower than for those grown in TIB. The lowest chlorophyll concentration was found in in vitro plantlets from CIB. We examined abnormally developed leaves, stems, and apical zones of in vitro plantlets that were produced in CIB. Among the three types, SS showed the highest stomatal density and the shortest stomatal length in in vitro plantlets. After acclimatization, plants from CIB exhibited the lowest values in biomass, such as height, root collar diameter, leaf fresh weight, leaf length, leaf width, petiole length, petiole diameter, and leaf area. Photosynthesis and transpiration rates of ex vitro plants were not significantly different among the three culture types, but stomatal conductance was higher in TIB than in the SS and CIB. Therefore, the results suggest that TIB is the preferable bioreactor to improve in vitro plantlet regeneration of L. tulipifera. TIB-originated plants showed higher growth rate than SS and CIB after transferring to soil.

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Improved propagation of vanilla (Vanilla planifolia Jacks. ex Andrews) using a temporary immersion system

Cited by 60 publications

References 23 publications

High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Effects ofin vitroculture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipiferaL.) from somatic embryos

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