Improved production of recombinant tumstatin in stably transformed Trichoplusia ni BTI Tn 5B1-4 cells

Chang, Kuo‐Hsuan; Lee, Jong Min; Jeon, Hee Kyoung; Chung, In Sik

doi:10.1016/j.pep.2004.01.004

Cited by 14 publications

(8 citation statements)

References 16 publications

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“…It will be necessary to investigate if this is an effect specific for the cell adhesion molecule L1, or if it is also valid in other cases. The production levels obtained with Tn-L1/Ig5-6 are within the range of those obtained for other proteins expressed in Tn cells using the stable expression system, for example, tumstatin which was obtained at approximately 4 mg l −1 (Chang et al, 2004), although a higher level of 41 mg l −1 has recently been described for ␤3-Nacetylglucosaminyltransferase 2 (Dojima et al, 2009). …”

Section: Discussionsupporting

confidence: 78%

“…However, there are several drawbacks to this system: since it is a lytic system cellular contents such as hydrolases, including proteases and glycosidases are released to the medium and might compromise protein quality; a large proportion of the protein is accumulated intracellularly since the folding and secretion machinery may be compromised by the infection. As an alternative, the stable expression system in insect cells has been used for the production of recombinant membrane (Kempf et al, 2002) and soluble glycoproteins (Barinka et al, 2004;Chang et al, 2002Chang et al, , 2004Gouveia et al, 2007;Swiech et al, 2008). For the secretory form of the enzyme fucosyltransferase III, the levels of protein obtained using the stable expression system were higher when compared with the baculovirus expression system (Morais and Costa, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…al., 2001), but it has been less used with the stable expression system (Chang et al, 2004;Dojima et al, 2009;Farrell et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…DMSO is a small amphipathic molecule soluble in both aqueous and organic media that exerts a wide variety of biological effects, by altering lipid membrane structure and metabolism, DNA synthesis and transcription, cell proliferation, differentiation and apoptosis (reviewed in Yu and Quinn, 1994). More specifically, DMSO was shown to increase production of recombinant proteins in stably transfected Drosophila melanogaster S2 (Chang et al, 2002;Swiech et al, 2008) and Tn insect cells (Chang et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Production and N-glycosylation of recombinant human cell adhesion molecule L1 from insect cells using the stable expression system. Effect of dimethyl sulfoxide

Gouveia

Kandzia²,

Conradt³

et al. 2010

Journal of Biotechnology

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

“…al., 2001), but it has been less used with the stable expression system (Chang et al, 2004;Dojima et al, 2009;Farrell et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production and N-glycosylation of recombinant human cell adhesion molecule L1 from insect cells using the stable expression system. Effect of dimethyl sulfoxide

Gouveia

Kandzia²,

Conradt³

et al. 2010

Journal of Biotechnology

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“…Exponentially growing TN-5B1-4 cells were transfected with pIZTx2/M1-M2 using the lipofectin method as described elsewhere [20]. Recombinant bacmids were produced with site-specific homologous recombination following the transformation of competent E. coli DH10Bac cells (Invitrogen) with pFastBac1/HA and pFastBac1/NA.…”

Section: Stable Transfection Of Tn-5b1-4 Cells and Generation Of Recomentioning

confidence: 99%

Production of influenza virus-like particles from stably transfected Trichoplusia ni BT1 TN-5B1-4 cells

Hong

Park

Lee

et al. 2015

Biotechnol Bioproc E

Self Cite

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We investigated the production of influenza A/ Korea/01-2-9/2009 (H1N1) virus-like particles (VLPs) containing four structural proteins, matrix protein 1 (M1), matrix protein 2 (M2), neuraminidase (NA) and hemagglutinin (HA), using stably transfected Trichoplusia ni BT1 TN-5B1-4 (TN-5B1-4) cells. Recombinant M1, M2, NA and HA were expressed as bands with molecular weights of 28, 17, 60, and 70 kDa, respectively, in stably transfected TN-5B1-4 (TN-5B1-4/M1-M2-NA-HA) cells. VLPs were purified from the culture medium of the TN-5B1-4/M1-M2-NA-HA cells by pelleting on a sucrose cushion, followed by ultracentrifugation in a sucrose density gradient. The four structural proteins were released together from the TN-5B1-4/M1-M2-NA-HA cells, and were co-purified from the same fractions of the sucrose density gradient, indicating that recombinant M1, M2, NA, and HA self-assembled into VLPs in the TN-5B1-4/M1-M2-NA-HA cells. Recombinant baculoviruses (rBac/NA and rBac/HA) expressing recombinant NA and HA were generated and used to co-infect stably transfected TN-5B1-4 (TN-5B1-4/M1-M2) cells expressing recombinant M1 and M2, resulting in the production of high-molecularweight VLPs and the release of self-assembled VLPs with diameters of 80 ~ 120 nm. The production of VLPs was greatly enhanced in the TN-5B1-4/M1-M2 cells co-infected with rBac/NA and rBac/HA, compared with the TN-5B1-4/M1-M2-NA-HA cells. Our results suggest that a stably transfected-insect cell expression system might be useful for producing VLPs for the development of recombinant vaccines against influenza.

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Targeting Drug Transporters – Combining In Silico and In Vitro Approaches to Predict In Vivo

Bahadduri

Polli

Swaan

et al. 2010

Methods in Molecular Biology

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Transporter proteins are expressed throughout the human body in different vital organs. They play an important role to various extents in determining absorption, distribution, metabolism, excretion, and toxicity (ADME/Tox) properties of therapeutic molecules. Over the past decade, numerous drug transporters have been cloned and considerable progress has been made toward understanding the molecular characteristics of individual transporters. In this chapter several in vitro and in silico techniques are described with applications to understand transporter behavior. These include employing new techniques to rapidly identify novel ligands for transporters. Ultimately these methods should lead to a greater overall appreciation of the role of transporters in vivo.

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Improved production of recombinant tumstatin in stably transformed Trichoplusia ni BTI Tn 5B1-4 cells

Cited by 14 publications

References 16 publications

Production and N-glycosylation of recombinant human cell adhesion molecule L1 from insect cells using the stable expression system. Effect of dimethyl sulfoxide

Production and N-glycosylation of recombinant human cell adhesion molecule L1 from insect cells using the stable expression system. Effect of dimethyl sulfoxide

Production of influenza virus-like particles from stably transfected Trichoplusia ni BT1 TN-5B1-4 cells

Targeting Drug Transporters – Combining In Silico and In Vitro Approaches to Predict In Vivo

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