Improved Production of Mashua (Tropaeolum tuberosum) Microtubers MAC-3 Morphotype in Liquid Medium Using Temporary Immersion System (TIS-RITA®)

Abstract

Essential molecules are embedded within the millenary crop Tropaeolum tuberosum (mashua); these compounds are critical for the Andean people’s traditional diet and extensively utilized by the pharmaceutical industry in Peru. In the Andean region, conventional cropping techniques generate microtubers susceptible to a viral infection, which substantially endangers mashua’s production. Therefore, we developed an innovative in vitro technique condition for enhancing the agriculture process for micro tubers production. The temporary immersion system (TIS) permits the production of high-quality microtubers in a reduced space, a lower amount of time, and in large quantities compared with tubers grown under traditional conditions. To obtain T. tuberosum’s microtubers via TIS, we propagated seedlings, utilizing TIS-RITA® vessels. A set of immersion frequency times were evaluated. Interestingly, results showed that immersion at 2 min every 3 h was more beneficial compared with 2 min every 5 h based on microtubers produced after 10 weeks from the treatments, revealing an efficient frequency setting which outputted improved microtubers quality and production.

GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays

“…The extraction of shoots with forceps from in vitro culture containers, as well as from TIS bioreactors, followed by arranging them on sterile surfaces in order to divide them with the help of surgical scalpels and their subsequent introduction into new culture containers, a process known as subculturing in the conventional in vitro culture process, is time-consuming and represents a significant portion the hand labor involved in most in vitro plant culture processes. The components of the actual GreenTray ® TIS bioreactor described herein, which is a further evolution of the ones presented in the past [31,32], make it significantly improved and versatile compared to other commercial TIS bioreactors, such as RITA ® , Matis ® , Plantform TM , SETIS TM [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17], or the TIS developed by several in vitro plant culture laboratories oriented to solve particular propagation objectives for diverse plant species [18][19][20][21][22][23][24][25][26][27][28].…”

“…Temporary immersion system (TIS) bioreactors have been developed over recent decades to favor plantlet or shoot development. The best-known commercial ones are RITA ® , its bigger version Matis ® [1][2][3][4][5][6][7], Plantform TM [8][9][10][11][12][13][14], and SETIS TM [15][16][17]. In addition, there are several other TISs developed for particular purposes by in vitro plant culture laboratories [18][19][20][21][22][23][24][25][26][27][28].…”

Sustainable Agriculture: Theories, Methods, Practices and Policies

“…Food supply is one of the important purposes of sustainable agricultural development. Gilmar et al [1] used an innovative in vitro technique to increase the cultivation of micro tubers, improve the yield and quality of seeds and crops, and ensure regional food supply.…”

El cultivo in vitro de plantas y su aporte en la agricultura local

“…Por ejemplo, en la actualidad escasos agronegocios aprovechan al cultivo in vitro como alternativa de propagación a gran escala de cultivos, entre los cuales solo se la utiliza en el café, banano, cacao, etcétera (Torres y Santos-Ordoñez, 2020). Sin embargo, existen pocos ejemplos de protocolos de cultivo in vitro de cultivos andinos como el mortiño, moringa, mashua, entre otras (Peña-Rojas et al, 2022;Cobo et al, 2018;Stephenson y Fahey, 2004). Esto hace que el cultivo de tejidos vegetales sea una tecnología poco explorada en el país y en sistemas agrícolas.…”