Improved production of -galactosidase and -fructofuranosidase by fungi using alternative carbon sources

Abstract

The influence of alternative carbon sources as inducers of -galactosidase and -fructofuranosidase by filamentous fungi (that are, Aspergillus aculeatus, Chrysonilia sitophila, Gliocladium virens, Aspergillus fumigatus and Trichoderma longibrachiatum), recently isolated from Brazil's Atlantic Forest biome has been investigated. The greatest levels of intracellular β-galactosidase activity were obtained using orange peel waste (56.31 U/mL) with A. aculeatus, rice straw (22.57 U/mL) with G. virens, sorghum straw (16.48 U/mL) with C. sitophila, and passion fruit peel with either A. fumigatus (17.26 U/mL) or T. longibrachiatum (17.53 U/mL). The most effective intracellular -fructofuranosidase activity was obtained by A. aculeatus using trub (409.46 U/mL) or passion fruit peel (44.59 U/mL). Thus, alternative carbon sources, such as orange peel and trub, exhibit great potential as inducers for the production of these enzymes. Such fungal isolates from the Atlantic Forest of Paraná, Brazil are promising candidates for generating significant amounts of β-galactosidase and -fructofuranosidase using abundant and inexpensive agro-industrial substrates.

Research Article Bioprospecting and enzymatic potential of filamentous fungi from the Bela Vista Biological Refuge in Itaipu, Brazil

“…From this perspective, some of these isolates reported have already been studied in the study of Andrades et al (2016), who investigated the influence of alternative carbon sources as inducers of β-galactosidase and fructofuranosidase by filamentous fungi Aspergillus aculeatus KM382062, Aspergillus fumigatus KY522978 and Trichoderma longibrachiatum KY548385. The highest levels of intracellular β-galactosidase activity were obtained using orange peel residues (56.31 U / mL) with A. aculeatus, A. fumigatus (17.26 U / mL) and T. longibrachiatum (17.53 U / mL).…”