Improved production of butyl butyrate with lipase from Thermomyces lanuginosus immobilized on styrene–divinylbenzene beads

Martins, Andréa Bercini; Friedrich, John L. R.; Cavalheiro, J. Coelho; Garcia‐Galan, Cristina; Barbosa, Oveimar; Ayub, Marco Antônio Záchia; Fernández‐Lafuente, Roberto; Rodrigues, Rafael C.

doi:10.1016/j.biortech.2013.02.052

Cited by 99 publications

(47 citation statements)

References 19 publications

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“…The highest yield (52.47 %) was achieved at 50 8C. Similar results were also reported by Martins [21] for the production of butyl butyrate via TLIM-catalyzed reaction. It is reported that an increase in temperature may cause a loss of enzyme activity [22].…”

Section: Effect Of Temperaturesupporting

confidence: 88%

Enzymatic Synthesis and Characterization of Sucrose Erucate

Jadhav¹,

Pratap²

2017

Tenside Surfactants Detergents

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The enzyme catalyzed synthesis is a environmentally friendly route compared to traditional syntheses. The lipase-catalyzed synthesis of sucrose erucate was achieved in a solvent mixture of t-butanol and dimethyl sulfoxide (4 : 1) by esterification of sucrose with erucic acid using the immobilized Thermomyces lanuginosus lipase. Various process parameters like temperature, substrate molar ratio, solvent, time, and enzyme loading were studied. The optimal conditions for the esterification reaction obtained were 10 %(w/w) enzyme loading, a molar ratio sucrose/fatty acid 1 : 1, mild reaction conditions (50 8C and atmospheric pressure) and reaction time (40 h with 55.6 % conversion). The sugar esters were characterized for surfactant properties at different concentration. Sucrose erucate showed a surface tension of (32.73 ± 0.01) mN m -1 at a critical micellar concentration of 9.8 · 10 -5 mol L -1 and good emulsification power.

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Section: Effect Of Temperaturesupporting

confidence: 88%

Enzymatic Synthesis and Characterization of Sucrose Erucate

Jadhav¹,

Pratap²

2017

Tenside Surfactants Detergents

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“…To ensure optimal biocatalyst activity and attain high yield reactions, several factors must be taken into consideration, mainly the temperature, water content and the loading of the biocatalyst in the reaction medium (MARTINS et al, 2013;FRIEDRICH et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Assessing the reaction conditions to mediate the milkfat-soybean oil enzymatic interesterification

Paula

Nunes

Castro

et al. 2016

Braz. J. Food Technol.

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Assessing the reaction conditions to mediate the milkfat-soybean oil enzymatic interesterification Determinação das condições reacionais para mediar a interesterificação enzimática da gordura do leite e do óleo de soja SummaryA food grade lipase from Rhizopus oryzae immobilized on a hybrid polysiloxane-polyvinyl alcohol matrix (SiO 2 -PVA) was used as the biocatalyst to mediate the interesterification reactions of a blend containing 65% milkfat and 35% soybean oil. All the reactions occurred in an inert nitrogen atmosphere in cylindrical glass reactors (80 mL) with 40 g of the milkfat-soybean oil blend. The influence of the following variables was evaluated: biocatalyst loading (250-1500 activity units per gram of blend), biocatalyst moisture content (5-20%), temperature (45-60 °C) and incubation time (2-48 h). The reactions were monitored by determining the free fatty acid content, triacylglycerol (TAGs) composition in carbon species, and the consistency of the interesterified (IE) products. The reaction conditions were set based on the parameters that provided a high interesterification yield and good consistency of the final product within the ideal range (200 to 800 gf cm -2 ). Hence the best results were obtained using a biocatalyst loading of 500 U g -1 of blend with 10% moisture content at 45 °C for 4 h. Under these conditions the consistency of the interesterified product was 539.7 ± 38 gf cm -2 . The results demonstrated the potential of the immobilized lipase to alter the TAGs profile of the milkfat-soybean oil blend, allowing for the production of structured lipids.Keywords: Rhizopus oryzae lipase; Milkfat; Soybean oil; Interesterification. ResumoLipase de grau alimentício de Rhizopus oryzae, imobilizada em uma matriz híbrida de polissiloxano-polivinilálcool (SiO 2 -PVA), foi usada como biocatalisador para mediar reações de interesterificação de uma mistura contendo 65% de gordura de leite e 35% de óleo de soja. Todas as reações ocorreram sob atmosfera inerte de nitrogênio, em reatores de vidro cilíndricos (80 mL) com 40 g da mistura. Avaliou-se a influência das seguintes variáveis: carregamento de biocatalisador (250-1.500 unidades de atividade por grama de mistura), umidade do biocatalisador (5-20%), temperatura (45-60 °C) e tempo de incubação (2-48 h). O progresso das reações foi monitorado pela determinação do conteúdo de ácidos graxos livres, da composição em triacilgliceróis (TAGs) em espécies de carbono e da consistência de produtos interesterificados (IE). As condições reacionais foram estabelecidas com base nos parâmetros que proporcionaram alto grau de interesterificação e consistência do produto final dentro da faixa ideal (200 a 800 gf cm -2 ). Neste contexto, os melhores resultados foram obtidos utilizando-se 500 U de biocatalisador por grama de mistura, com 10% de...

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“…Results show that all preparations presented similar performances, producing approximately 70% yield of synthesis in the short reaction time of 4 h. Under optimized conditions, the commercial Lipozyme TL-IM and the MCI Gel CHP20P support immobilized with 120 mg g -1 of TLL, showed yields around 60 and 80% of butyl butyrate after 4 h, respectively. 42 Commercial Lipozyme TL-IM derivative showed lower than 20% fatty acid ethyl esters yields when used with olive and palm oil. 43 These results demonstrate the potential applicability of the support Immobead 150 with TLL immobilized via multipoint attachment for esterification and transesterification reactions.…”

Section: Reactions Of Esterification and Transesterificationmentioning

confidence: 97%