Improved positive electrospray ionization of patulin by adduct formation: Usefulness in liquid chromatography–tandem mass spectrometry multi-mycotoxin analysis

Malysheva, Svetlana V.; Mavungu, José Diana Di; Boonen, Jente; Spiegeleer, Bart De; Goryacheva, Irina Yu.; Vanhaecke, Lynn; Saeger, Sarah De

doi:10.1016/j.chroma.2012.10.060

Cited by 29 publications

(12 citation statements)

References 23 publications

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“…It indicated that patulin was reacted with ring opening reaction with PPL. The fragment at m/z 159 can alternatively undergo successive losses of carbon dioxide ( Malysheva et al, 2012 ). The speculation corresponds to the previous study by UV scanning.…”

Section: Resultsmentioning

confidence: 99%

Effective Biodegradation of Mycotoxin Patulin by Porcine Pancreatic Lipase

2018

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Patulin is a common contaminant in fruits and vegetables, which is difficult to remove. In this study, the biodegradation of patulin using porcine pancreatic lipase (PPL) was investigated. The method of HPLC was used to analyze the concentration of patulin. Batch degradation experiments were performed to illustrate the effect of PPL amount, pH, temperature, contact time, and initial concentration. Besides, the degradation product of patulin was characterized by full wavelength scanning and MS technologies. The results showed that the optimum degradation conditions of PPL for patulin was observed at pH 7.5, 40°C for 48 h. The percentage of degradation could reach above 90%. The structure of degradable product of patulin was inferred by the molecular weight 159.0594, named C7H11O4+. It indicated that PPL was effective for the degradation of patulin in fruits and vegetables juice.

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Section: Resultsmentioning

confidence: 99%

Effective Biodegradation of Mycotoxin Patulin by Porcine Pancreatic Lipase

2018

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“…Lohrey et al, (2013) synthesised isotopically-labelled 13 C 2 -tenuazonic acid for use as an internal standard for use in an LC-MS/MS method for tenuazonic acid in tomato products, but employed LC-HRMS to characterise the labelled material and establish its purity. Patulin which is another simple, small molecule mycotoxin has been analysed in urine as a methanol adduct principally using LC-MS/MS, although HRMS was utilised to reliable assignment of the patulin fragment ions (Malysheva et al, 2012).…”

Section: Monitoring Natural Toxins Including Mycotoxins In Food and Feedmentioning

confidence: 99%

Future perspectives in Orbitrap™-high-resolution mass spectrometry in food analysis: a review

Şenyuva

Gökmen

Sarikaya

2015

Food Additives & Contaminants: Part A

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A literature search from 2007 to 2014 was conducted to identify publications where principally LC-Orbitrap™-high-resolution mass spectrometry (HRMS) has been employed in food analysis. Of a total of 212 relevant references, only 22 papers were from 2007-10, but in subsequent years there has been a steady growth in publications with 38-55 relevant papers being published each year from 2011 to 2014. In the food safety area, over 50% of the published papers were equally divided between pesticides, veterinary drug residues and natural toxins (including mycotoxins) focused primarily on multi-analyte target analysis. LC-Orbitrap-HRMS was also found to be increasingly important for the analysis of bioactive substances, principally phenolic compounds in foods. A number of studies reported for the first time the identification of new fungal metabolites, predominantly various conjugated forms of known mycotoxins. Novel process contaminants were also identified by LC-Orbitrap-HRMS, as were various substances used for food adulteration and bioactive substances in herbal products and dietary supplements. Untargeted analysis is seen as a major future trend where HRMS plays a significant role. Retrospective analysis of scanned high-resolution mass spectra in conjunction with relevant databases can provide new insights. Metabolomics is also being increasingly used where foods are being profiled through fingerprinting using HRMS. All evidence points towards future growth in the number of applications of HRMS in food safety and quality, as the power of this technique gains wider recognition.

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“…With the increasing availability of MS detectors, variants of MS have also been used for quantification of PAT. Most such methods have combined LC and MS. Variations have included stable isotope dilution assay (Rychlik & Schieberle 1999), atmospheric pressure chemical ionisation (Sewram et al 2000), time-offlight MS (Gilbert & Senyuva 2008), tandem MS (Desmarchelier et al 2011;Malysheva et al 2012;Xiang et al 2012; ultra-high-performance LC (UPLC) with tandem MS (Beltrán et al 2014;Vaclavikova et al 2015), and recently dopant-assisted atmospheric pressure photo-ionisation with tandem MS (DA-APPI; Zhang et al 2014). GC can also be combined with MS for PAT detection upon derivatisation (Kharandi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Quantification of patulin in fruit leathers by ultra-high-performance liquid chromatography-photodiode array (UPLC-PDA)

Maragos

Busman

et al. 2015

Food Additives & Contaminants: Part A

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Patulin is a mycotoxin commonly found in certain fruit and fruit products. For this reason many countries have established regulatory limits pertaining to, in particular, apple juice and apple products. Fruit leathers are produced by dehydrating fruit puree, leaving a sweet product that has a leathery texture. A recent report in the literature described the detection of patulin at substantial levels in fruit leathers. To investigate this further, an ultra-high-performance liquid chromatography-photodiode array (UPLC-PDA) method was developed for the sensitive detection of patulin in fruit leathers. Investigations were also made of the suitability of direct analysis in real time-mass spectrometry (DART-MS) for detection of patulin from the surface of fruit leathers. Results indicated DART-MS was insufficiently sensitive for quantification from the surface of home-style apple leathers, although patulin spiked onto the surface of leather or peel could be detected. The UPLC-PDA method was used to determine the fate of patulin during the preparation of home-made fruit leathers. Interestingly, when a home-style process was used, the patulin was not destroyed, but rather increased in concentration as the puree was dehydrated. The UPLC-PDA method was also used to screen for patulin in commercial fruit leathers. Of the 36 products tested, 14 were above the limit of detection (3.5 μg kg(-1)) and nine were above the limit of quantification (12 μg kg(-1)). Positive samples were confirmed by UPLC-MS/MS. Only one sample was found above the US regulatory limit for single-strength apple juice products (50 μg kg(-1)). These results suggest patulin can be concentrated during preparation and can be found in fruit leathers. The limited survey suggests that patulin is fairly prevalent in such commercial products, but that the levels are usually low.

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Improved positive electrospray ionization of patulin by adduct formation: Usefulness in liquid chromatography–tandem mass spectrometry multi-mycotoxin analysis

Cited by 29 publications

References 23 publications

Effective Biodegradation of Mycotoxin Patulin by Porcine Pancreatic Lipase

Effective Biodegradation of Mycotoxin Patulin by Porcine Pancreatic Lipase

Future perspectives in Orbitrap™-high-resolution mass spectrometry in food analysis: a review

Quantification of patulin in fruit leathers by ultra-high-performance liquid chromatography-photodiode array (UPLC-PDA)

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