Improved photodynamic inactivation of gram‐positive bacteria using hematoporphyrin encapsulated in liposomes and micelles

Tsai, Tsung‐Han; Yu, Yang; Wang, Tse Hsien; Chien, Hsiung–Fei; Chen, Chin-Tin

doi:10.1002/lsm.20754

Cited by 85 publications

(66 citation statements)

References 34 publications

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“…Previously, we showed that pharmaceutical delivery systems can be used to facilitate PDI due to improved photochemical efficiency and the subsequent microbe-killing effect (24). In this study, we further found that one of the most commonly studied biomaterials, chitosan, can potentiate the PDI efficacy in planktonic cells and biofilms.…”

supporting

confidence: 55%

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Chitosan Augments Photodynamic Inactivation of Gram-Positive and Gram-Negative Bacteria

Tsai

Chien

Wang

et al. 2011

Antimicrob Agents Chemother

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Antimicrobial photodynamic inactivation (PDI) was shown to be a promising treatment modality for microbial infections. This study explores the effect of chitosan, a polycationic biopolymer, in increasing the PDI efficacy against Gram-positive bacteria, including Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, and methicillin-resistant S. aureus (MRSA), as well as the Gram-negative bacteria Pseudomonas aeruginosa and Acinetobacter baumannii. Chitosan at <0.1% was included in the antibacterial process either by coincubation with hematoporphyrin (Hp) and subjection to light exposure to induce the PDI effect or by addition after PDI and further incubation for 30 min. Under conditions in which Hp-PDI killed the microbe on a 2-to 4-log scale, treatment with chitosan at concentrations of as low as 0.025% for a further 30 min completely eradicated the bacteria (which were originally at ϳ10 8 CFU/ml). Similar results were also found with toluidine blue O (TBO)-mediated PDI in planktonic and biofilm cells. However, without PDI treatment, chitosan alone did not exert significant antimicrobial activity with 30 min of incubation, suggesting that the potentiated effect of chitosan worked after the bacterial damage induced by PDI. Further studies indicated that the potentiated PDI effect of chitosan was related to the level of PDI damage and the deacetylation level of the chitosan. These results indicate that the combination of PDI and chitosan is quite promising for eradicating microbial infections.

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confidence: 55%

“…Preparation and characterization of micelles. Hp was encapsulated in micelles by a reverse-phase evaporation method as described before (24). Briefly, 0.5 mg of Hp and 100 mg of PF127 were dissolved in a cosolvent system (chloroformmethanol at 4:1, vol/vol), which was then removed by rotary evaporation to form a thin film.…”

Section: Methodsmentioning

confidence: 99%

Chitosan Augments Photodynamic Inactivation of Gram-Positive and Gram-Negative Bacteria

Tsai

Chien

Wang

et al. 2011

Antimicrob Agents Chemother

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“…As described, aPDT is expected to be a potent method for bacterial removal without using antibiotics. Recently, numerous studies in vitro have demonstrated that bacteria can be effectively killed by aPDT, [13][14][15][16][17] and this study showed that it is possible to remove mycoplasmas from cell cultures using aPDT. However, the efficiency of aPDT in killing bacteria that have host cell-invasive properties is still unknown.…”

Section: Discussionmentioning

confidence: 95%

Mycoplasma Removal from Cell Culture Using Antimicrobial Photodynamic Therapy

Hasebe

Ishikawa

Shamsul³

et al. 2013

Photomedicine and Laser Surgery

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Objective: The objective of this research was to determine the effectiveness of antimicrobial photodynamic therapy (aPDT) in the removal of mycoplasmas from contaminated cells. Background data: Mycoplasmas often contaminate cell cultures. The cell-contaminating mycoplasmas are removed by antibiotics, but the use of antibiotics usually induces antibiotic-resistant bacteria. aPDT is expected to be a possible alternative to antibiotic treatments for suppressing infections. Materials and Methods: Mycoplasma salivarium (Ms)-infected human embryonic kidney (HEK) 293 cells were irradiated using a red light-emitting diode (LED) in the presence of methylene blue (MB) as a photosensitizer. The Ms viable count was determined using culture on agar plates or using a mycoplasma detection kit. Results: aPDT performed using red LED irradiation was effective in decreasing live Ms in the presence of MB without damaging the HEK293 cells. aPDT removed live Ms from the infected cells after washing the cells with sterilized phosphate-buffered saline (PBS) to decrease the initial number of live Ms before aPDT. Conclusions: This study suggests that aPDT could remove mycoplasmas from contaminated cells.

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“…We will give some examples of nanostructures that have been investigated as PS-delivery systems. As the main application of aPDT is likely to be in the medical (wound and surfaces sterilization) and environmental fields (food industry and water purification), particular interest has been placed on biocompatible and biodegradables nanomatrix, such as polymeric nanoparticles [45], micelles [46], and liposomes [47]. The use of biodegradable polymeric nanoparticles as PS-delivery nanoparticles has been recently reported.…”

Section: Photomedicine -Advances In Clinical Practicementioning

confidence: 99%

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

Bloise¹,

Minzioni²,

Imbriani³

et al. 2017

Photomedicine - Advances in Clinical Practice

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Recent developments in light-controlled therapies (e.g., photodynamic and photothermal therapies) provide promising strategies to prevent and suppress bacterial infections, which are a leading cause of morbidity and mortality. Antibacterial photodynamic therapy (aPDT) has drawn increasing attention from the scientific society for its potential to kill multidrug-resistant pathogenic bacteria and for its low tendency to induce drug resistance. In this chapter, we summarize the mechanism of action of aPDT, the photosensitizers, as well the current developments in terms of treating Gram-positive and Gram-negative bacteria. The chapter also describes the recent progress relating to photomedicine for preventing bacterial infections and biofilm formation. We focus on the laser device used in aPDT and on the light-treatment parameters that may have a strong impact on the results of aPDT experiments. In the last part of this chapter, we survey on the various nanoparticles delivering photoactive molecules, and photoactive-nanoparticles that can potentially enhance the antimicrobial action of aPDT.

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Improved photodynamic inactivation of gram‐positive bacteria using hematoporphyrin encapsulated in liposomes and micelles

Abstract: Our results indicate that photosensitizer entrapped in micelle exert similar or better PDI efficacy than that of liposome, which indicates this formulation may be useful for the treatment of local infections in the future.

Cited by 85 publications

References 34 publications

Chitosan Augments Photodynamic Inactivation of Gram-Positive and Gram-Negative Bacteria

Chitosan Augments Photodynamic Inactivation of Gram-Positive and Gram-Negative Bacteria

Mycoplasma Removal from Cell Culture Using Antimicrobial Photodynamic Therapy

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

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