Improved photodynamic action of nanoparticles loaded with indium (III) phthalocyanine on MCF-7 breast cancer cells

Souto, Carlos Augusto Zanoni; Madeira, Klesia Pirola; Rettori, Daniel; Baratti, Mariana Ozello; Rangel, Letícia Batista Azevedo; Razzo, Daniel; Silva, André Romero da

doi:10.1007/s11051-013-1879-8

Cited by 12 publications

(5 citation statements)

References 44 publications

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“…Such fine explorations could be expanded both at biological and chemical level, this avenue being a tempting and pristine new one assessment in PDT field. Thus, at biological level our study discloses new possibilities for testing its efficacy in other cancers types such as malignant melanoma [ 51 ] or breast cancer [ 52 ], generally accessible tumors for delivering a particular PS in a targeted manner. In addition, the chemistry approaches of In-Pc prevails over its biological assessments and reach new levels by exploring for instance PEGylated poly(d,l-lactide-co-glycolide) nanoparticles loaded with In-Pc in an attempt to increase the PDT efficacy using MCF7 breast cancer cell line [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Toxicological and efficacy assessment of post-transition metal (Indium) phthalocyanine for photodynamic therapy in neuroblastoma

et al. 2016

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Metallo-phthalocyanines due to their photophysical characteristics as high yield of triplet state and long lifetimes, appear to be good candidates for photodynamic therapy (PDT). Complexes with diamagnetic metals such as Zn2+, Al3+ Ga3+ and In3+meet such requirements and are recognized as potential PDT agents. Clinically, Photofrin® PDT in neuroblastoma therapy proved in pediatric subjects diagnosed with progressive/recurrent malignant brain tumors increased progression free survival and overall survival outcome. Our study focuses on the dark toxicity testing of a Chloro-Indium-phthalocyanine photosensitizer (In-Pc) upon SH-SY5Y neuroblastoma cell line and its experimental in vitro PDT. Upon testing, In-Pc has shown a relatively high singlet oxygen quantum yield within the cells subjected to PDT (0.553), and 50 μg/mL IC50. Classical toxicological and efficacy assessment were completed with dynamic cellular impedance measurement methodology. Using this technology we have shown that long time incubation of neuroblastoma cell lines in In-Pc (over 5 days) does not significantly hinder cell proliferation when concentration are ≤ 10 μg/mL. When irradiating neuroblastoma cells loaded with non-toxic concentration of In-Pc, 50% of cells entered apoptosis. Transmission electron microscopy has confirmed apoptotic characteristics of cells. Investigating the proliferative capacity of the in vitro treated cells we have shown that cells that “escape” the irradiation protocol, present a reduced proliferative capacity. In conclusion, In-Pc represents another photosensitizer that can display sound PDT properties enhancing neuroblastoma therapy armentarium.

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Section: Discussionmentioning

confidence: 99%

Toxicological and efficacy assessment of post-transition metal (Indium) phthalocyanine for photodynamic therapy in neuroblastoma

et al. 2016

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“…Souto et al [ 50 ] reported indium (III) phthalocyanine (InPc, Scheme 6 ) loaded into nanoparticles consisting of poly(ethylene glycol)- b -poly(D, L-lactide- co -glycolide) copolymer for breast cancer treatment. Interestingly, the confocal laser scanning microscopy studies showed that free InPc as well as InPc-loaded micelles were similarly internalized into cancer cells.…”

Section: Phthalocyanine-polymeric Nanoparticle Delivery Systems For Cancer Photodynamic Therapymentioning

confidence: 99%

Recent Progress in Phthalocyanine-Polymeric Nanoparticle Delivery Systems for Cancer Photodynamic Therapy

2021

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This perspective article summarizes the last decade’s developments in the field of phthalocyanine (Pc)-polymeric nanoparticle (NP) delivery systems for cancer photodynamic therapy (PDT), including studies with at least in vitro data. Moreover, special attention will be paid to the various strategies for enhancing the behavior of Pc-polymeric NPs in PDT, underlining the great potential of this class of nanomaterials as advanced Pcs’ nanocarriers for cancer PDT. This review shows that there is still a lot of research to be done, opening the door to new and interesting nanodelivery systems.

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“…They evaluated the photodynamic activity of ZnPc incorporated in unilamellar liposomal formulations containing increasing concentrations of cholesterol that improved the stability of the particles, optimized the release of ZnPc, and modulated its phototoxicity against several human tumor cells. PEGylated poly(d,l-lactide-co-glycolide) nanoparticles (NPs) were employed to encapsulate indium(III) phthalocyanine (InPc) and its PDT efficacy against MCF-7 breast tumor cells was evaluated by Souto et al [175]. It was demonstrated in this way that other factors than the chemical composition of the polymeric shell, such as the phthalocyanine concentration, incubation time, and laser power, can modulate the therapeutic effects.…”

Section: Formulation Techniquesmentioning

confidence: 99%

Porphyrin Derivative Nanoformulations for Therapy and Antiparasitic Agents

et al. 2020

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Porphyrins and analogous macrocycles exhibit interesting photochemical, catalytic, and luminescence properties demonstrating high potential in the treatment of several diseases. Among them can be highlighted the possibility of application in photodynamic therapy and antimicrobial/antiparasitic PDT, for example, of malaria parasite. However, the low efficiency generally associated with their low solubility in water and bioavailability have precluded biomedical applications. Nanotechnology can provide efficient strategies to enhance bioavailability and incorporate targeted delivery properties to conventional pharmaceuticals, enhancing the effectiveness and reducing the toxicity, thus improving the adhesion to the treatment. In this way, those limitations can be overcome by using two main strategies: (1) Incorporation of hydrophilic substituents into the macrocycle ring while controlling the interaction with biological systems and (2) by including them in nanocarriers and delivery nanosystems. This review will focus on antiparasitic drugs based on porphyrin derivatives developed according to these two strategies, considering their vast and increasing applications befitting the multiple roles of these compounds in nature.

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Improved photodynamic action of nanoparticles loaded with indium (III) phthalocyanine on MCF-7 breast cancer cells

Cited by 12 publications

References 44 publications

Toxicological and efficacy assessment of post-transition metal (Indium) phthalocyanine for photodynamic therapy in neuroblastoma

Toxicological and efficacy assessment of post-transition metal (Indium) phthalocyanine for photodynamic therapy in neuroblastoma

Recent Progress in Phthalocyanine-Polymeric Nanoparticle Delivery Systems for Cancer Photodynamic Therapy

Porphyrin Derivative Nanoformulations for Therapy and Antiparasitic Agents

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