“…Among those, 140 SAAVs in 131 proteins occurred only in NM patients (Figure 2D; Table S4), and 223 proteins in 18 MT patients had 256 SAAVs, of which 110 SAAVs in 100 proteins occurred in both NM and MT samples (Figure 2D; Table S5), and 203 SAAVs in 184 proteins only occurred in MT samples (Figure 2D; Table S6). Similarly to single nucleotide variants (SNVs), some mutations reported previously, such as those in TP53, antigen-presenting cells (APCs), vascular endothelial growth factor (VEGF), and SMAD4 were found 39, 40, 41, 42, 43. Among these, two mRNA-protein positively changed and metabolism-related proteins, FDFT1, a 47-kDa membrane-associated enzyme located at a branch point in the mevalonate pathway involved in the replication stage of the hepatitis virus C (HCV) life cycle 44 and paclitaxel sensitivity in hypopharynx cancer cell, 45 and UQCR5, a component of the ubiquinol-cytochrome c reductase complex, amplifying in primary breast cancer core biopsy samples46, 47, 48 and overexpressing in gastric cancer, 49 were found to be an occurrence of somatic alterations, which was validated with Sanger sequencing, in the 18.2% (8/44) and 9.1% (4/44) of CRC, and are specific to CLM (Figure 3A).…”