Improved Paclitaxel and Baccatin III Production in Suspension Cultures of Taxusmedia

Abstract

A cell suspension culture of Taxus media was established from a stable callus line of this species. The growth rate and production of paclitaxel and baccatin III of this cell suspension were significantly increased during the shake flask culture in its respective optimum media for cell growth and product formation, which were selected after assaying 24 different culture media. The highest yields of paclitaxel (2.09 mg L(-1)) and baccatin III (2.56 mg L(-1)) in the production medium rose (factors of 7.0 and 3.0, respectively) in the presence of methyljasmonate (220 microg g(-1) FW). When the elicitor was added together with mevalonate (0.38 mM) and N-benzoylglycine (0.2 mM), the increase in the yields of paclitaxel and baccatin III was even higher (factors of 8.3 and 4.0, respectively). Thereafter, a two-stage culture for cell suspension was carried out using a 5-l stirred bioreactor running for 36 days, the first stage being in the cell growth medium until cells entered their stationary growth phase (12 days) and the second stage being in the production medium supplemented with the elicitor and two putative precursors in the concentrations indicated above. Under these conditions, 21.12 mg L(-1) of paclitaxel and 56.03 mg L(-1) of baccatin III were obtained after 8 days of culture in the production medium.

A reliable protocol for the stable transformation of non-embryogenic cells cultures of grapevine (Vitis vinifera L.) and Taxus x media

“…Longitudinally-halved stem sections were placed in such a way that the inner cut surface came into contact with the culture medium optimized for calli induction [43]. The obtained cell lines were maintained in solid cultures in the optimum growth medium, as described elsewhere [44]. Calli were routinely subcultured every 14 days.…”

Effects of immobilization by entrapment in alginate and scale‐up on paclitaxel and baccatin III production in cell suspension cultures of Taxus baccata

“…Another possibility is the yield of paclitaxel and its synthetically useful progenitors in Taxus cell culture systems, described in several reports (Ketchum et al, 1999(Ketchum et al, , 2003Phisalaphong and Linden, 1999;Yukimune et al, 2000;Linden and Phisalaphong, 2000;Son et al, 2000;Wang et al, 2001;Cusidó et al, 2002).…”

TAXOIDES: METABOLITOS SECUNDARIOS DEL ÁRBOL DEL TEJO (Taxus spp.)

“…cuspidata 0.11 Son et al (2000) T. media 0.53 Cusidó et al (2002) T. wallichiana 0.75 Navia-Osorio et al (2002) T. chinensis 1.50 Wang et al (2001) T. yunnanensis 1.90 Zhang et al (2002) T. baccata 2.71 Bentebibel et al (2005) 7-epi-10-diacetyl taxol (KetchumTandon, Gibson, Begley, & Shuler, 1999), and 10-diacetyl taxol in T. baccata (Vander et al, 1994), among others. Table 2 shows the production rate of taxol in in vitro cultures of Taxus spp.…”

“…cuspidata 0.11 Son et al (2000) T. media 0.53 Cusidó et al (2002) T. wallichiana 0.75 Navia-Osorio et al (2002) T. chinensis 1.50 Wang et al (2001) T. yunnanensis 1.90 Zhang et al (2002) T. baccata 2.71 Bentebibel et al (2005) …”