Improved packing of preparative biochromatography columns by mechanical vibration

Abstract

The bioprocessing industry relies on packed-bed column chromatography as its primary separation process to attain the required high product purities and fulfill the strict requirements from regulatory bodies. Conventional column packing methods rely on flow packing and/or mechanical compression. In this work, the application of ultrasound and mechanical vibration during packing was studied with respect to packing density and homogeneity. We investigated two widely used biochromatography media, incompressible ceramic hydroxyapatite, and compressible polymethacrylatebased particles, packed in a laboratory-scale column with an inner diameter of 50 mm.

Single-Molecule Dynamics Reflect IgG Conformational Changes Associated with Ion-Exchange Chromatography

“…We consider two possible explanations for this apparent discrepancy: the presence of morphological differences inside the commercially available optimized chromatographic column and the quasi-chromatographic flow chamber constructed for single-molecule experiments. It is commonly accepted that microstructural packing is important for efficient chromatographic performance . Factors such as slurry solvent and concentration, packing density, and packing method can significantly affect the quality of a separation.…”

“…It is commonly accepted that microstructural packing is important for efficient chromatographic performance. 65 Factors such as slurry solvent 66 and concentration, 67 packing density, 68 and packing method 69 can significantly affect the quality of a separation. Better efficiency, for example, can be achieved by using higher slurry concentrations resulting in suppressing particle size segregation inside the column.…”