Improved osteoblast cell affinity on plasma-modified 3-D extruded PCL scaffolds

Domingos, Marco; Intranuovo, Francesca; Gloria, Antonio; Gristina, Roberto; Ambrosio, Luigi; Bartolo, Paulo Jorge Da Silva; Favia, Pietro

doi:10.1016/j.actbio.2012.12.031

Cited by 164 publications

(149 citation statements)

References 38 publications

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“…Therefore plasma is very adequate to treat delicate structures such as nanofibers. Moreover, the action of plasma treatments is limited in depth to few nanometers below the surface thus not affecting the bulk properties of the base material that conserves its mechanical stability [41,74,114]. Plasma treatment has the potential to homogenously modify the surface of complex shaped biomaterials which makes it even more attractive for the functionalization of nanofibers [115,116].…”

Section: Different Functionalization Approaches Of Electrospun Biodegmentioning

confidence: 99%

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

Ghobeira¹,

Morent²

2017

Biodegradable Polymers: Recent Developments and New Perspectives

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Section: Different Functionalization Approaches Of Electrospun Biodegmentioning

confidence: 99%

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

Ghobeira¹,

Morent²

2017

Biodegradable Polymers: Recent Developments and New Perspectives

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“…It was previously reported that after polyesters were undergone O 2 plasma treatment, the polymeric surfaces became etched and more hydrophilic due to the presence of newly generated polar components, for example, ACAOA, >C@O, and ACOOH, on the surfaces. [23][24][25][26] This resulted in the increases in the O/C atomic ratios of the plasma-treated blended scaffolds.…”

Section: Characterization Of Scaffoldsmentioning

confidence: 95%

PCL/PHBV blended three dimensional scaffolds fabricated by fused deposition modeling and responses of chondrocytes to the scaffolds

Kosorn

Sakulsumbat

Uppanan

et al. 2016

J. Biomed. Mater. Res.

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In this study, poly(ε-caprolactone)/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PCL/PHBV) blended porous scaffolds were fabricated by fused deposition modeling (FDM). PCL/PHBV filaments, initially prepared at different weight ratios, that is, 100/0, 75/25, 50/50, and 25/75, were fabricated by the lay-down pattern of 0/90/45/135° to obtain scaffolds with dimension of 6.0 × 6.0 × 2.5 mm and average filament diameters and channel sizes in the ranges of 370-390 µm and 190-210 µm, respectively. To enhance the surface hydrophilicity of the materials, the scaffolds were subsequently subjected to a low pressure oxygen plasma treatment. The untreated and plasma-treated scaffolds were comparatively characterized, in terms of surface properties, mechanical strength, and biological properties. From SEM, AFM, water contact angle, and XPS results, the surface roughness, wettability, and hydrophilicity of the blended scaffolds were found to be enhanced after plasma treatment, while the compressive strength of the scaffolds was scarcely changed. It was, however, found to increase with an increasing content of PHBV incorporated. The porcine chondrocytes exhibited higher proliferative capacity and chondrogenic potential when being cultured on the scaffolds with greater PHBV contents, especially when they were plasma-treated. The PCL/PHBV scaffolds were proven to possess good physical, mechanical, and biological properties that could be appropriately used in articular cartilage regeneration. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1141-1150, 2017.

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“…31 Extruded PCL scaffolds were also plasma-treated with a C 2 H 4 /N 2 deposition, followed by H 2 posttreatment, to coat the surface of 3D structures with a nitrogen-rich film containing groups to enhance the scaffold's hydrophilicity and cell affinity. 32 Furthermore, iron-doped hydroxyapatite nanoparticles were embedded in a PCL matrix to develop fully biodegradable nanocomposite 2D substrates for bone tissue engineering. 33 Therefore, the combination of structural and functional modifications for PCL scaffolds/substrates can enrich the properties of PCL-based 3D scaffolds for cell culture studies and tissue/organ regeneration.…”

Section: Discussionmentioning

confidence: 99%

Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells

Hong¹,

Bang²,

Xu³

et al. 2015

IJN

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Controlling the thickness of an electrospun nanofibrous scaffold by altering its pore size has been shown to regulate cell behaviors such as cell infiltration into a three-dimensional (3D) scaffold. This is of great importance when manufacturing tissue-engineering scaffolds using an electrospinning process. In this study, we report the development of a novel process whereby additional aluminum foil layers were applied to the accumulated electrospun fibers of an existing aluminum foil collector, effectively reducing the incidence of charge buildup. Using this process, we fabricated an electrospun scaffold with a large pore (pore size >40 μm) while simultaneously controlling the thickness. We demonstrate that the large pore size triggered rapid infiltration (160 μm in 4 hours of cell culture) of individual endothelial progenitor cells (EPCs) and rapid cell colonization after seeding EPC spheroids. We confirmed that the 3D, but not two-dimensional, scaffold structures regulated tubular structure formation by the EPCs. Thus, incorporation of stem cells into a highly porous 3D scaffold with tunable thickness has implications for the regeneration of vascularized thick tissues and cardiac patch development.

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Improved osteoblast cell affinity on plasma-modified 3-D extruded PCL scaffolds

Cited by 164 publications

References 38 publications

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

PCL/PHBV blended three dimensional scaffolds fabricated by fused deposition modeling and responses of chondrocytes to the scaffolds

Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells

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Improved osteoblast cell affinity on plasma-modified 3-D extruded PCL scaffolds

Cited by 164 publications

References 38 publications

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

Plasma surface functionalization of biodegradable electrospun scaffolds for tissue engineering applications

PCL/PHBV blended three dimensional scaffolds fabricated by fused deposition modeling and responses of chondrocytes to the scaffolds

Thickness-controllable electrospun fibers promote tubular structure formation by&nbsp;endothelial progenitor cells

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Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells