Improved organogenesis and micro-structural traits in micropropagated plantlets of Caralluma umbellata Haw. in response to Meta-Topolin

Jayaprakash, K.; Manokari, M.; Cokulraj, M.; Dey, Abhijit; Faisal, Mohammad; Alatar, Abdulrahman A.; Joshee, Nirmal; Shekhawat, Mahipal S.

doi:10.1007/s11240-023-02447-0

Cited by 2 publications

(3 citation statements)

References 52 publications

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“…Regarding the acclimatization stage, no significant differences were observed for the effect of CK on acclimatization percentage, but a slightly higher response in explants cultured with m-T was observed. These results agreed with those observed in Aloe polyphylla, S. cumini, and C. umbellate, where the plantlets induced with m-T were successfully acclimatized [39,56,57]. The reason for this may be due to the rapid uptake and transport of mT into the plant system and the production of reversibly sequestered metabolites [57].…”

Section: Discussionsupporting

confidence: 89%

“…These results agreed with those observed in Aloe polyphylla, S. cumini, and C. umbellate, where the plantlets induced with m-T were successfully acclimatized [39,56,57]. The reason for this may be due to the rapid uptake and transport of mT into the plant system and the production of reversibly sequestered metabolites [57].…”

Section: Discussionsupporting

confidence: 89%

“…Similar to our results, Naaz et al [56], studying Syzygium cumini, reported that shoots induced with m-T increased rhizogenic competency compared with shoots coming from kinetin, 2-isopentyl adenine, or BA. Similarly, in Caralluma umbellata, shoots derived from a culture medium supplemented with m-T and NAA showed the highest in vitro rooting activity [57]. In contrast to our work, in S. sempervirens and Juniperus drupacea, growing shoots with m-T did not improve the rooting response [10,58].…”

Section: Discussioncontrasting

confidence: 88%

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Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Rojas-Vargas

Montalbán²,

Moncaleán³

2023

Forests

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Cryptomeria japonica (Thunb. ex L.f.) D. Don is a commercial tree native to Japan and is one of the most important forest species in that country and the Azores (Portugal). Because of the quality of C. japonica timber, several genetic improvement programs have been performed. Recently, some studies focusing on C. japonica somatic embryogenesis have been carried out. However, in this species, this process uses immature seeds as initial explants, and for this reason, it is not possible to achieve the maximum genetic gain (100% genetic of the donor plant). Although some studies have been made applying organogenesis to this species, the success of the process in adult trees is low. For this reason, our main goal was to optimize the micropropagation method by using trees older than 30 years as a source of plant material. In this sense, in a first experiment, we studied the effect of different types of initial explants and three basal culture media on shoot induction; then, two sucrose concentrations and two light treatments (LEDs versus fluorescent lights) were tested for the improvement of rooting. In a second experiment, the effects of different plant growth regulators (6-benzylaminopurine, meta-topolin, and thidiazuron) on shoot induction and the subsequent phases of the organogenesis process were analyzed. The cultures produced the highest number of shoots when QL medium (Quoirin and Lepoivre, 1977) and long basal explants (>1.5 cm) were used; the shoots obtained produced a higher number of roots when they were grown under red LED lights. Moreover, root induction was significantly higher in shoots previously induced with meta-topolin.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 89%

Section: Discussioncontrasting

confidence: 88%

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Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Rojas-Vargas

Montalbán²,

Moncaleán³

2023

Forests

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Meta-Topolin-induced mass shoot multiplication and biosynthesis of valuable secondary metabolites in Stevia rebaudiana Bertoni bioreactor culture

Ptak,

Szewczyk,

Simlat

et al. 2023

Sci Rep

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Stevia rebaudiana Bertoni possesses various medicinal and food industrial applications. This study is the first to explore the effect of the cytokinins meta-Topolin (mT; 6-(3-hydroxybenzylamino) purine), zeatin, kinetin, and BAP (6-benzylaminopurine) at concentrations of 0 (control), 5, 10, and 15 µM on shoot multiplication, as well as stevioside, rebaudioside A, phenolic acid, and flavonoid content in bioreactor cultures. The highest number of shoots (23.4 per explant) was obtained in the medium containing 5 μM of mT. However, 15 μM of mT was superior for fresh biomass production and dry biomass accumulation. Reversed-phase (RP)-HPLC analysis showed a beneficial effect of 5 μM mT on stevioside (11.43 mg/g dry weight [DW]) and rebaudioside A (10.74 mg/g DW) biosynthesis. In all conditions, the ratio of rebaudioside A/stevioside ranged from 0.75 to 1.12. The phenolic acids chlorogenic, neochlorogenic, isochlorogenic A, and rosmarinic were confirmed in the stevia extracts, as were the flavonoids isoquercetin, and quercitrin. The highest accumulations of chlorogenic and neochlorogenic acids and flavonoids were observed in shoot tissues derived from 5 µM mT, whereas 5 µM of BAP stimulated biosynthesis of chlorogenic, isochlorogenic A, and rosmarinic acids. This is the first report on the use of mT-cytokinin showing high potential in stevia cultures.

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Improved organogenesis and micro-structural traits in micropropagated plantlets of Caralluma umbellata Haw. in response to Meta-Topolin

Cited by 2 publications

References 52 publications

Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Meta-Topolin-induced mass shoot multiplication and biosynthesis of valuable secondary metabolites in Stevia rebaudiana Bertoni bioreactor culture

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