Improved n- butanol production from lignocellulosic hydrolysate by Clostridium strain screening and culture-medium optimization

Magalhães, Beatriz L.; Grassi, Maria Carolina B.; Pereira, Gonçalo Amarante Guimarães; Brocchi, Marcelo

doi:10.1016/j.biombioe.2017.10.044

Cited by 40 publications

(17 citation statements)

References 21 publications

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“…There is ongoing research on metabolic engineering to develop clostridial strains capable of simultaneously fermenting hexose and pentose for butanol production [71, 93, 94, 113, 114]. Even though Lee et al [23] stated metabolic engineering is necessary for simultaneous utilization of sugars, researchers have developed different feeding and pre-growth strategies achieving co-utilization without any strain manipulation [73, 115–120]. However, in the mixed sugar fermentation study of Zhang et al [121], transcriptional studies suggested that glucose inhibition on xylose metabolism-related genes was still present despite the simultaneous utilization of glucose and xylose.…”

Section: Fermentative Butanol Production From Mixed Sugarsmentioning

confidence: 99%

Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysis

Birgen

Dürre

Preisig

et al. 2019

Biotechnol Biofuels

103

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After just more than 100 years of history of industrial acetone–butanol–ethanol (ABE) fermentation, patented by Weizmann in the UK in 1915, butanol is again today considered a promising biofuel alternative based on several advantages compared to the more established biofuels ethanol and methanol. Large-scale fermentative production of butanol, however, still suffers from high substrate cost and low product titers and selectivity. There have been great advances the last decades to tackle these problems. However, understanding the fermentation process variables and their interconnectedness with a holistic view of the current scientific state-of-the-art is lacking to a great extent. To illustrate the benefits of such a comprehensive approach, we have developed a dataset by collecting data from 175 fermentations of lignocellulosic biomass and mixed sugars to produce butanol that reported during the past three decades of scientific literature and performed an exploratory data analysis to map current trends and bottlenecks. This review presents the results of this exploratory data analysis as well as main features of fermentative butanol production from lignocellulosic biomass with a focus on performance indicators as a useful tool to guide further research and development in the field towards more profitable butanol manufacturing for biofuel applications in the future. Electronic supplementary material The online version of this article (10.1186/s13068-019-1508-6) contains supplementary material, which is available to authorized users.

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Section: Fermentative Butanol Production From Mixed Sugarsmentioning

confidence: 99%

Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysis

Birgen

Dürre

Preisig

et al. 2019

Biotechnol Biofuels

103

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“…Since the 1960s, cellulase has been used increasingly in food, paper, pulp, textile industries, and pharmaceutical industries. However, there are limited studies that exploited the hydrolysis process of agricultural residues of lignocellulosic materials to produce high-value products with low cost [10][11][12]. Moreover, the problem with the biorefining process is that it is costly due to a lack of biocatalysts that are capable of withstanding a variety of environmental stressors such as low/high temperature, acidic/alkaline pH, high salinity, and expression of multi-enzyme complexes.…”

Section: Introductionmentioning

confidence: 99%

Coconut Mesocarp-Based Lignocellulosic Waste as a Substrate for Cellulase Production from High Promising Multienzyme-Producing Bacillus amyloliquefaciens FW2 without Pretreatments

et al. 2022

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Facing the crucial issue of high cost in cellulase production from commercial celluloses, inexpensive lignocellulosic materials from agricultural wastes have been attractive. Therefore, several studies have focused on increasing the efficiency of cellulase production by potential microorganisms capable of secreting a high and diversified amount of enzymes using agricultural waste as valuable substrates. Especially, extremophilic bacteria play an important role in biorefinery due to their high value catalytic enzymes that are active even under harsh environmental conditions. Therefore, in this study, we aim to investigate the ability to produce cellulase from coconut-mesocarp of the potential bacterial strain FW2 that was isolated from kitchen food waste in South Korea. This strain was tolerant in a wide range of temperature (–6–75 °C, pH range (4.5–12)) and at high salt concentration up to 35% NaCl. The molecular weight of the purified cellulase produced from strain FW2 was estimated to be 55 kDa. Optimal conditions for the enzyme activity using commercial substrates were found to be 40–50 °C, pH 7.0–7.5, and 0–10% NaCl observed in 920 U/mL of CMCase, 1300 U/mL of Avicelase, and 150 U/mL of FPase. It was achieved in 650 U/mL, 720 U/mL, and 140 U/mL of CMCase, Avicelase, and FPase using coconut-mesocarp, respectively. The results revealed that enzyme production by strain FW2 may have significant commercial values for industry, argo-waste treatment, and other potential applications.

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“…As a consequence, production of SCOAs was prevalent over solvents not only in experiments with glucose of this work, but also in those using synthetic vinasse. In fact, acidogenic fermentation tends to be the prevailing one especially at low concentrations of sugars in substrate (MONOT et al , 1982; MAGALHÃES et al , 2018). Additionally, it is noteworthy that even at 60 g Gli L −1 of initial concentration of carbon source, there are technical literature reports on cases of poor butanol generation (3.7 mg BuOH L −1 ) in a fermentation medium under pH controlled at 6.8 and 5.0 (GEORGE & CHEN, 1983).…”

Section: Resultsmentioning

confidence: 99%

Acidogenic Fermentation of Sugarcane Vinasse and Glucose Using Two Different Anaerobic Digestion Inocula

Martins

Araujo

Mockaitis

et al. 2021

Preprint

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Vinasse is an agroindustrial wastewater obtained at large amounts in countries such as Brazil, which is one of the world greatest sugar and ethanol producers from sugarcane. Despite its most common use is as nutrient for fertirrigation in sugarcane crops nowadays, alternative processing has been intensely sought in last decades for vinasse aiming primarily the production of energy carriers and high-added value chemicals. One of these alternatives is the anaerobic digestion promoted by bacteria, which results in several products of economic importance. In view of these information, this work presents an investigation of acidogenic fermentation by C. acetobutylicum ATCC 824 and C. beijerinckii ATCC 25752 was conducted in batch reactors by using synthetic vinasse and aqueous glucose solution as substrates. Evaluation of effects of microorganism and carbon source was focused mainly on the production of short chain organic acids (SCOAs), although other metabolites, such as ethanol, were also detected. Carbohydrates were effectively consumed by bacteria, so remaining concentrations of sucrose and glucose (Suc and Glu) in both substrates were considerably low at the end of tests. Lactic acid (HLa) was the prevailing metabolite in all experiments, determining the trends observed for total SCOAs content as a function of incubation time, while other acids were generated in much lesser amounts. Moreover, ethanol (EtOH) attained notably higher concentrations in batch reactors containing glucose as substrate, which showed more relevant production of this chemical. Maximum of 14.1 g HLa L-1 was quantified for lactic acid at incubation time 96 h in reactor using synthetic vinasse and C. beijerinckii (VB), linked to a productivity P=146.46 mg HLa L-1 h-1 and a yield Y=1108.03 mg HLa g Suc-1. For ethanol, maximum was 1.6 g EtOH L-1, occurring in reactor containing glucose and C. acetobutylicum (GA) also at incubation time 90 h, resulting in P=13.60 mg EtOH L-1 h-1 and Y=73.04 mg EtOH g Glu-1. Finally, kinetic models were adjusted to experimental data of carbohydrate content, lactate concentration and optical density (indicative of microorganism growth), showing good prediction capability as suggested by values of fit quality parameters such as Residual Sum of Squares (RSS) and R2.

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Improved n- butanol production from lignocellulosic hydrolysate by Clostridium strain screening and culture-medium optimization

Cited by 40 publications

References 21 publications

Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysis

Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysis

Coconut Mesocarp-Based Lignocellulosic Waste as a Substrate for Cellulase Production from High Promising Multienzyme-Producing Bacillus amyloliquefaciens FW2 without Pretreatments

Acidogenic Fermentation of Sugarcane Vinasse and Glucose Using Two Different Anaerobic Digestion Inocula

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