Improved Muscle Mitochondrial Capacity Following Gastric Bypass Surgery in Obese Subjects

Fernström, Maria; Bakkman, Linda; Loogna, Peter; Rooyackers, Olav; Svensson, Madeleine; Jakobsson, Towe; Brandt, Lena; Lagerros, Ylva Trolle

doi:10.1007/s11695-015-1932-z

Cited by 21 publications

(16 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, we observed that FCCP-induced maximal respiration was compromised in myotubes established from severely obese humans but did not improve following RYGB surgery. These data are in agreement with previous reports showing that reduced mitochondrial respiratory capacity was imprinted in skeletal muscle cells derived from severely obese humans [33][34][35][36][37] and RYGB surgery was not able to reverse it 34,[38][39][40] . This observation, along with improvements in mitochondrial network morphology and dynamics protein Drp1 activation at 7-months after RYGB, suggests that alterations in mitochondrial dynamics may precede improvements in mitochondrial respiratory capacity.…”

Section: Discussionsupporting

confidence: 93%

“…Although mitochondrial uncoupled maximal respiration was not changed after RYGB surgery, it is important to point out that the results from our study does not rule out other improvements related to mitochondrial function. It has been reported that ADP-stimulated State III respiration was improved after weight loss surgery 34,38,39 . Thus, future studies should seek to use more comprehensive approaches to assess mitochondrial function in primary myotubes from RYGB patients.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Roux-en-Y gastric bypass surgery restores insulin-mediated glucose partitioning and mitochondrial dynamics in primary myotubes from severely obese humans

Kugler

Gundersen

et al. 2019

Int J Obes

View full text Add to dashboard Cite

Background/Objectives: Impaired insulin-mediated glucose partitioning is an intrinsic metabolic defect in skeletal muscle from severely obese humans (BMI ≥ 40 kg/m 2). Roux-en-Y gastric bypass (RYGB) surgery has been shown to improve glucose metabolism in severely obese humans. The purpose of the study was to determine the effects of RYGB surgery on glucose partitioning, mitochondrial network morphology, and markers of mitochondrial dynamics skeletal muscle from severely obese humans. Subject/Methods: Human skeletal muscle cells were isolated from muscle biopsies obtained from RYGB patients (BMI = 48.0 ± 2.1, n=7) prior to, 1-month and 7-months following surgery and lean control subjects (BMI = 22.4 ± 1.1, n=7). Complete glucose oxidation, non-oxidized glycolysis rates, mitochondrial respiratory capacity, mitochondrial network morphology and regulatory proteins of mitochondrial dynamics were determined in differentiated human myotubes. Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 97%

Roux-en-Y gastric bypass surgery restores insulin-mediated glucose partitioning and mitochondrial dynamics in primary myotubes from severely obese humans

Kugler

Gundersen

et al. 2019

Int J Obes

View full text Add to dashboard Cite

show abstract

“…Another longitudinal study in the same clinical population as the present study has recently been published . In that study, the patients were followed 6 months post‐operatively and the authors reported an increased state 3 respiration and P/O ratio following surgery.…”

Section: Discussionmentioning

confidence: 73%

Mitochondrial respiratory capacity remains stable despite a comprehensive and sustained increase in insulin sensitivity in obese patients undergoing gastric bypass surgery

et al. 2018

View full text Add to dashboard Cite

show abstract

“…On the other hand, the effects on mitochondrial function seem to be dependent of intervention time, since after 6 months of endurance training increases genes expression entails in oxidative phosphorylation, but mitochondrial mass and function were not assessed . Also, the improved mitochondrial respiration may be related by weight loss, as was found after 18 months bariatric surgery, as well as other studies after bariatric surgery found to increase mitochondrial coupling and ATP generation in skeletal muscle, a known effect of physical training in this tissue . Studies in skeletal muscle have shown that mitochondrial respiration is lower in individuals with obesity compared to normal weight ones .…”

Section: Discussionmentioning

confidence: 95%

Physical training, UCP1 expression, mitochondrial density, and coupling in adipose tissue from women with obesity

Brandão

Carvalho

Souza

et al. 2019

Scandinavian Med Sci Sports

View full text Add to dashboard Cite

Background: Exercise training may improve energy expenditure, thermogenesis, and oxidative capacities. Therefore, we hypothesized that physical training enhances white adipose tissue mitochondrial oxidative capacity from obese women. Objective: To evaluate mitochondrial respiratory capacity, mitochondrial content, and UCP1 gene expression in white adipose tissue from women with obesity before and after the physical training program. Methods: Women (n = 14, BMI 33 ± 3 kg/m 2 , 35 ± 6 years, mean ± SD) were submitted to strength and aerobic exercises (75%-90% maximum heart rate and multiple repetitions), 3 times/week during 8 weeks. All evaluated subjects were paired, before and after training for resting metabolic rate (RMR), substrate oxidation (lipid and carbohydrate) by indirect calorimeter, deuterium oxide body composition, and aerobic maximum velocity (V max ) test. At the beginning and at the ending of the protocol, abdominal subcutaneous adipose tissue was collected to measure the mitochondrial respiration by high-resolution respirometry, mitochondrial content by citrate synthase (CS) activity, and UCP1 gene expression by RT-qPCR. Results: Combined physical training increased RMR, lipid oxidation, and V max but did not change body weight/composition. In WAT, exercise increased CS activity, decreased mitochondrial uncoupled respiration and mRNA of UCP1. RMR was positively correlated with fat-free mass. Conclusion: Physical training promotes an increase in mitochondrial content without changing tissue respiratory capacity, a reduction in mitochondrial uncoupling degree and UCP1 mRNA expression in WAT. Finally, it improved the resting metabolic rate, lipid oxidation and physical performance, independent of the body changing free, or fat mass in obese women. K E Y W O R D Scitrate synthase, human energy metabolism, mitochondria, obesity, physical training, uncoupling protein 1, white adipose tissue

show abstract

Improved Muscle Mitochondrial Capacity Following Gastric Bypass Surgery in Obese Subjects

Cited by 21 publications

References 40 publications

Roux-en-Y gastric bypass surgery restores insulin-mediated glucose partitioning and mitochondrial dynamics in primary myotubes from severely obese humans

Roux-en-Y gastric bypass surgery restores insulin-mediated glucose partitioning and mitochondrial dynamics in primary myotubes from severely obese humans

Mitochondrial respiratory capacity remains stable despite a comprehensive and sustained increase in insulin sensitivity in obese patients undergoing gastric bypass surgery

Physical training, UCP1 expression, mitochondrial density, and coupling in adipose tissue from women with obesity

Contact Info

Product

Resources

About