Improved micropropagation, morphometric traits and photosynthetic pigments content using liquid culture system in Spathoglottis plicata Blume

Shekhawat, Mahipal S.; Rangan, Latha; Priyadharshini, S.; Manokari, M.

doi:10.1007/s42535-021-00303-0

Cited by 7 publications

(3 citation statements)

References 30 publications

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“…The maximum response for callus, leaf and root formation, and root length were witnessed with the inoculation of 100 somatic embryos per TIS. Uma et al [141] used varied inoculum densities (3,6, and 12 explants) per TIS to ascertain the optimum inoculum density for the best shooting response in bananas. The TIS with explant densities of 3 and 6 generated higher shoots (24 shoots/explants) than those with inoculum densities of 12 explants.…”

Section: Important Factors Influencing Temporary Immersion System (Tis)mentioning

confidence: 99%

“…Orchids are amazingly stunning ornamental plants belonging to the Orchidaceae family, comprising 30,000-35,000 species, numerous hybrids, and varieties [1][2][3]. Most orchids carry a high price in the international floriculture market as they have great floricultural appeal with their diversely colored attractive flowers having varied fragrances, shapes, and sizes [4][5][6]. Orchids also possess good therapeutic properties despite their outstanding ornamental values due to the rich contents of beneficial phytochemicals [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

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Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation.

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Section: Important Factors Influencing Temporary Immersion System (Tis)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

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“…Carnevali, J.L. Tapia & I. Ramírez (Bello-Bello et al 2020) and Spathoglottis plicata Blume (Shekhawat et al 2021), which have also proven to be a tool to produce orchids for conservation purposes (Jiang et al 2017).…”

Section: Introductionmentioning

confidence: 99%

In vitro germination and development of “Canelita” (Lycaste aromatica (Graham) Lindl.) in gravity immersion bioreactors

Solís-Zanotelli

Baltazar-Bernal

Cruz-Huerta

et al. 2022

In Vitro Cell.Dev.Biol.-Plant

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The orchid Lycaste aromatica (Graham) Lindl. is known for its outstanding cinnamon aroma, which has caused its illegal extraction from its natural habitat. For this reason, procedures were developed for the asymbiotic seeds germination and seedlings development in a gravity immersion bioreactor (GIB) system. Four culture media were tested for in vitro germination: ½ Murashige and Skoog (MS), MS, ½ Knudson C 15 g L − 1 sucrose, Knudson C. The e ciency of the in vitro culture protocol using the semi-solid medium was compared and with four immersion frequencies of the GIB system in a liquid medium, using 30 mL of ½ MS medium per seedling. Germination began at eight weeks and lasted for six weeks. The best treatment was ½ MS with 161 germinated seeds. In 90 days, the cultures in bioreactors reached signi cant differences in the number of leaves (10 leaves), number of roots (4.7 roots), and seedling height (5.4 cm). However, the seedlings developed in the semi-solid medium reported a higher number of shoots (1.6 shoots) and root length (2 cm); furthermore, the bioreactor culture showed the highest photosynthesis rate (0.74 µmol CO 2 g − 1 s − 1 ). The culture system in bioreactors is an excellent alternative to develop this species because it increases photosynthesis and the size of the seedlings, which favor their acclimatization. Key MessageLycaste aromatica begins germination in vitro at 8 weeks, lasts for six weeks, develops functional stomata, and promotes photosynthesis in the gravity immersion bioreactor.

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Micropropagation of Brassavola nodosa (L.) Lindl. using SETIS™ bioreactor

Vendrame

Beleski

2023

Plant Cell Tiss Organ Cult

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Brassavola nodosa (L.) Lindl. is a tropical epiphytic orchid showing characteristics of interest for the ornamental nursery industry. However, problems with traditional propagation methods limit the development of a large-scale commercial production system. In addition, this species is considered endangered due to the reduction in population caused by habitat destruction, climate change and overcollection from native areas. The use of micropropagation has been investigated for this species, and the use of liquid in vitro systems showed potential for use of temporary immersion bioreactors for micropropagation of B. nodosa. This study evaluated the e ciency of the SETIS™ bioreactor system for the micropropagation of B. nodosa by adjusting parameters of immersion (frequency and duration) and by comparing it to conventional semi-solid culture systems. Results indicate that temporary immersion of B. nodosa explants with a frequency of 2 h and duration of 2 min returned the highest multiplication rates, with 4.6 shoots produced per explant compared with 2.8 shoots per explant in semi-solid agarbased systems. The use of bioreactors also promoted increased growth and development and in vitro rooting, therefore improving survival and facilitating acclimatization of in vitro-derived plantlets. This is the rst study demonstrating a successful protocol for large-scale micropropagation of B. nodosa using SETIS™ bioreactors, which could have signi cant value and impact for the commercial production of this species as well as for conservation purposes. Key MessageMicropropagation of Brassavola nodosa was achieved using SETIS temporary immersion bioreactors, showing good multiplication rates, increased growth and development and in vitro rooting, thus improving survival and acclimatization ex vitro.

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Improved micropropagation, morphometric traits and photosynthetic pigments content using liquid culture system in Spathoglottis plicata Blume

Cited by 7 publications

References 30 publications

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

In vitro germination and development of “Canelita” (Lycaste aromatica (Graham) Lindl.) in gravity immersion bioreactors

Micropropagation of Brassavola nodosa (L.) Lindl. using SETIS™ bioreactor

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