Improved methods for isolation and enumeration of Malassezia furfur from human skin

Abstract

A medium for the isolation and enumeration of Malassezia furfur is described. Incubation at 34°C yielded geometric mean counts (in CFU per square centimeter) of 2.6 x 103 on the forehead, 8.5 x 102 on the cheek, and 9.6 x 103 on the back. These counts compared favorably with microscopic counts and greatly exceeded those obtained with previously described media.

No apparent association between periocular and ocular microcolonization and the degree of inflammation in patients with atopic keratoconjunctivitis

“…Three cilia were taken from the inferior lid margin using sterile pincers. One was placed on a hematin agar plate for isolation of aerobic bacteria, one on a Leeming & Notman agar (LNA) plate [8] for isolation of Malassezia species, and one on a CHROMagar Candida plate (CHROMagar Co., Paris, France) for isolation of Candida species. Samples for aerobic bacteria were taken from the inferior and superior eyelid margins with a sterile cotton tipped swab moistened with 0.9% saline and from the lower and superior conjunctival fornices with an unmoistened swab (Copan, Brescia, Italy) which were placed in transport tubes (Copan).…”

In vitromodulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule ofMalasseziaspecies

“…Therefore, the aim of this study was to determine the effect of the capsule of Malassezia on production of primary keratinocyte-derived proinflammatory (IL-1a, IL-6, IL-8 and TNF-a) and antiinflammatory (IL-10) cytokines to help elucidate the yeast's role in the pathogenesis of inflammatory skin diseases, including SD. (Leeming & Notman, 1987) at 34 1C in a humidified, aerobic atmosphere. For experiments, Malassezia were cultivated using a modified Leeming & Notman's broth containing 1% (w/v) , 2% (v/v) olive oil (Sigma), 200 mg cyclohemimide mL À1 and 25 mg chloramphenicol mL À1 , aerobically at 34 1C in an orbital incubator (120 r.p.m.).…”

Adhesion of Staphylococcus aureus and Staphylococcus epidermidis to the EpiskinR reconstructed epidermis model and to an inert 304 stainless steel substrate

“…The cutaneous flora, estimated at ca 10 6 cells cm )2 (Leyden et al 1987), is principally made up of coryneform bacteria (Corynebacterium, Brevibacterium and Propionibacterium) and staphylococci. Acinetobacter and Micrococcus are also found, but to a lesser extent, together with fungal flora comprised mainly of the Malassezia genus (Noble 1974;Leeming and Notman 1987;Leeming et al 1989;Roth Rudolf and James William 1989). Breakdown of this microbial equilibrium may lead to the proliferation of endogenous micro-organisms and/or contamination by exogenous micro-organisms, giving rise to more or less extensive infectious disorders.…”