Improved method for the determination of propofol in blood by high-performance liquid chromatography with fluorescence detection

Plummer, Greg

doi:10.1016/0378-4347(87)80394-8

Cited by 302 publications

(118 citation statements)

References 1 publication

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…Hence, it was decided to apply the typical procedure of propofol determination by a simpler and more reliable HPLC with fluorescence detection [13] rather than the GC procedure requiring propofol derivatization [11] . As seen in Figure 1, the main difference between the investigated systems is the level of unbound propofol percentage.…”

Section: Resultsmentioning

confidence: 99%

Influence of intralipid on free propofol fraction assayed in human serum albumin solutions and human plasma

Kalitynski

Dawidowicz

Poszytek

2006

Acta Pharmacologica Sinica

View full text Add to dashboard Cite

Aim: It is generally assumed that only unbound drugs can reach the site of action by diffusing across the membranes and exerting pharmacological effects by interacting with receptors. Recent research has shown that the percentage of free drugs may depend on the total drug concentration. The aim of the paper is to verify whether the mentioned dependence reported for propofol also takes place in plasma and human serum albumin samples in the presence of intralipid --the medium used as a vehicle for propofol infusions and a parenteral nutrition agent. Methods: Artificial plasma samples and human plasma were spiked with intralipid or ethanolic solutions of propofol. The samples were then assayed for free propofol concentration using ultrafiltration and high performance liquid chromatography with fluorimetric detection. Results: The decrease of the total drug concentration results in free propofol fraction increase, irrespectively of the used type of propofol solvent and sample type. The addition of intralipid causes the lowering of the overall free drug fraction with respect to the samples spiked with ethanolic solutions of the drug. Conclusion: The presence of intralipid does not influence the phenomenon of free propofol fraction rise at low total drug concentration. Such a rise cannot be ignored in clinical conditions when the drug is applied for sedative, antiemetic or other low-dosage purposes.

show abstract

Section: Resultsmentioning

confidence: 99%

Influence of intralipid on free propofol fraction assayed in human serum albumin solutions and human plasma

Kalitynski

Dawidowicz

Poszytek

2006

Acta Pharmacologica Sinica

View full text Add to dashboard Cite

show abstract

“…Blood samples for propofol measurement by highperformance liquid chromatography [13] were taken from an indwelling intravenous cannula in the contralateral arm. Between one and four samples were taken from each patient at various target concentrations to assess the accuracy of the TCI system.…”

Section: Methodsmentioning

confidence: 99%

Patient‐maintained propofol sedation

1997

View full text Add to dashboard Cite

SummaryWe have developed a system which allows patients to operate a target-controlled infusion of propofol to provide sedation and we have studied its use in 36 unpremedicated patients undergoing local and regional anaesthetic procedures lasting 10-280 min. An intravenous propofol infusion was started at a target plasma level of 1 mg.ml ¹1 . The patient was able to increase the target propofol concentration in 0.2-mg.ml ¹1 increments by pressing a demand button. There was a lockout interval of 2 min and a maximum permissible target concentration of 3 mg.ml¹1 . There was considerable interindividual variability in propofol consumption (mean 39.3 mg.kg ¹1 .min ¹1 , range 3-131 mg.kg ¹1 .min ¹1 ), no cardiovascular instability and little oversedation. Eight patients required supplementary oxygen. Optimal sedation was provided at median target concentrations of 0.8-0.9 mg.ml ¹1 . The target-controlled infusion system bias was -47% and the inaccuracy was 48%. Patient satisfaction was high and 89% said that they would definitely use the technique again. This technique combines the benefits of target-controlled infusion with patient-controlled feedback and produces safe intra-operative sedation.

show abstract

“…Continuous monitoring included ECG, invasive arterial blood pressure (via a 20 G cannula in the radial artery) and pulse oximetry. Arterial blood samples were drawn every 3 h for measurement of arterial blood gases and estimation of blood propofol concentrations using High Pressure Liquid Chromatography with fluorescence detection [7] until the propofol infusion was discontinued.…”

Section: Cuse Historymentioning

confidence: 99%