Improved method for preparation of samples for the polymerase chain reaction for detection of Coxiella burnetii in milk using immunomagnetic separation

Muramatsu, Yasukazu; Maruyama, Minoru; Yanase, Tsuyoshi; Ueno, Hiroshi; Morita, Çhiharu

doi:10.1016/0378-1135(95)00203-0

Cited by 23 publications

(24 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Coxiella burnetii has already been detected using different PCR methods in the placenta and milk samples of infected dairy cows [24,26,34]. But, to our knowledge, this study is the first report of direct identification of Coxiella burnetii by PCR in the faeces of naturallyinfected dairy cows.…”

Section: Discussionmentioning

confidence: 88%

“…Moreover, bacteriological isolation requires confined level-3 laboratories and cannot be performed for mass-screening. In the last few years, conventional polymerase chain reaction (PCR) has become a very useful method for the detection of Coxiella burnetii DNA in several biological samples taken from sheep and goats [7,26,28,31]. In dairy cattle, this technique has been evaluated for the detection of Coxiella burnetii only in milk [24,34].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Shedding routes ofCoxiella burnetiiin dairy cows: implications for detection and control

et al. 2006

View full text Add to dashboard Cite

-Reliable detection of Coxiella burnetii shedders is a critical point for the control of the spread of this bacterium among animals and from animals to humans. Coxiella burnetii is shed by ruminants mainly by birth products (placenta, birth fluids), but may also be shed by vaginal mucus, milk, and faeces, urine and semen. However, the informative value of these types of samples to identify shedders under field conditions is unknown. Our aim was then to describe the responses obtained using a real-time PCR technique applied to milk, vaginal mucus and faeces samples taken from 242 dairy cows in commercial dairy herds known to be naturally infected with Coxiella burnetii, and to assess their putative associations. Positive results were found in all types of tested samples even in faeces. No predominant shedding route was identified. Among the shedder cows, 65.4% were detected as shedders by only one route. By contrast, cows with positive results for all three samples were scarce (less than 7%). Testing a cow based on only one type of biological sample may lead to misclassify it with regards to its shedding of Coxiella burnetii and thereby underestimate the risk of bacterial spread within a herd. dairy cows / Coxiella burnetii / shedding routes / real-time PCR / Q fever

show abstract

Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Shedding routes ofCoxiella burnetiiin dairy cows: implications for detection and control

et al. 2006

View full text Add to dashboard Cite

show abstract

“…In the last decade, the polymerase chain reaction (PCR) has become a very useful method for Coxiella burnetii DNA detection in different biological samples taken from ruminants [6,21,24,26,35]. Recently, real-time PCR was developed with the aim of estimating the bacterial burden in samples.…”

Section: Introductionmentioning

confidence: 99%

Coxiella burnetiishedding by dairy cows

Guatteo

Beaudeau

Joly³

et al. 2007

Vet. Res.

144

142

View full text Add to dashboard Cite

-While shedding routes of Coxiella burnetii are identified, the characteristics of Coxiella shedding are still widely unknown, especially in dairy cattle. However, this information is crucial to assess the natural course of Coxiella burnetii infection within a herd and then to elaborate strategies to limit the risks of transmission between animals and to humans. The present study aimed at (i) describing the characteristics of Coxiella burnetii shedding by dairy cows (in milk, vaginal mucus, faeces) in five infected dairy herds, and at (ii) investigating the possible relationships between shedding patterns and serological responses. A total of 145 cows were included in a follow-up consisting of seven concomitant samplings of milk, vaginal mucus, faeces and blood (Day 0, D7, D14, D21, D28, D63, D90). Detection and quantification of Coxiella burnetii titres were performed in milk, vaginal mucus and faeces samples using real-time PCR assay, while antibodies against Coxiella were detected using an ELISA technique. For a given shedding route, and a given periodicity (weekly or monthly), cows were gathered into different shedding kinetic patterns according to the sequence of PCR responses. Distribution of estimated titres in Coxiella burnetii was described according to shedding kinetic patterns. Coxiella burnetii shedding was found scarcely and sporadically in faeces. Vaginal mucus shedding concerned almost 50% of the cows studied and was found intermittently or sporadically, depending on the periodicity considered. Almost 40% of cows were detected as milk shedders, with two predominant shedding patterns: persistent and sporadic, regardless of the sampling periodicity. Significantly higher estimated titres in Coxiella burnetii were observed in cows with persistent shedding patterns suggesting the existence of heavy shedder cows. These latter cows were mostly, persistently highly-seropositive, suggesting that repeated serological testings could be a reliable tool to screen heavy shedders, before using PCR assays. dairy cow / Coxiella burnetii / shedding / antibodies / kinetics

show abstract

“…Milk samples for the PCR were prepared as described elsewhere [13]. The nested PCR was performed as described previously [19].…”

mentioning

confidence: 99%

Prevalence of Coxiella burnetii Infection in Dairy Cattle with Reproductive Disorders.

Htwe

Kako

et al. 1998

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The prevalence of Coxiella burnetii infection in 207 cattle with reproductive disorders was studied by using an indirect immunofluorescence (IF) test, nested polymerase chain reaction (PCR) and isolation. IF antibodies to phase I and phase II antigens of C. burnetii were found in 122 (58.9%) and 125 (60.4%) of the sera, respectively, and PCR-positives were found in 8 (3.9%) of the sera and in 51 (24.6%) of the milk samples. In addition, C. burnetii was isolated from 51 (24.6%) of the milk samples by inoculating laboratory mice. The results indicate that the IF test plus PCR are useful in the diagnosis of bovine coxiellosis. It is difficult to deny that dairy cattle with reproductive disorders would be one of the important reservoirs of C. burnetii responsible for infection in both animal and human populations in Japan. -KEY WORDS: bovine coxiellosis, Coxiella burnetii, nested polymerase chain reaction.

show abstract

Improved method for preparation of samples for the polymerase chain reaction for detection of Coxiella burnetii in milk using immunomagnetic separation

Cited by 23 publications

References 10 publications

Shedding routes ofCoxiella burnetiiin dairy cows: implications for detection and control

Shedding routes ofCoxiella burnetiiin dairy cows: implications for detection and control

Coxiella burnetiishedding by dairy cows

Prevalence of Coxiella burnetii Infection in Dairy Cattle with Reproductive Disorders.

Contact Info

Product

Resources

About