Improved Method for Performing Density-Gradient Electrophoresis

Electrophoresis in a density gradient

“…It seems probable, however, that the usefulness of the method will depend largely both on the reproducibility of the separations which can be obtained and on the shapes of bands after separation, with particular reference to degree of resolution. The results recently published by Berg & Beeler (1958) show an apparently good resolution for human serum and plasma, although, as they themselves state, the method of protein estimation used must have led to an exaggeration of the albumin peaks. As appears in Fig.…”

Chromatography

“…Modifications. There have been some interesting modifications of the electromigration procedure: a portable apparatus (261), electromigration followed by chromatography in a strip of paper (268), use of acrylamide gel as a supporting medium (3)1), use and recovery of cellulose acetate migration medium (188), an improved method for performing density-gradient electrophoresis (36), the use of permselective, ion exchange electrodes (317), and the use of a cell with ion exchange membrane partitions (59). Discontinuous systems of buffers (159,333) and an electrophoretic method for desalting amino acids have been described (30)).…”

“…The effect of sorption upon the mobility of ions and the separation of cesium, thallium, sodium, copper, zinc, and cadmium ions in acetic acid and in ammonia solutions has been studied (25). Various radioactive species formed by neutron irradiation of substances, such as phosphates, have been separated by electrochromatography in paper (187,363,36)). Many oxy acids of phosphorus including those with P-O-P and P-P bonds have also been separated in paper (363).…”