Improved method for impedance measurements of mammalian cells

Rümenapp, Christine; Remm, Maido; Wolf, Bernhard; Gleich, Bernhard

doi:10.1016/j.bios.2009.02.030

Cited by 24 publications

(15 citation statements)

References 17 publications

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“…The option of distinguishing between free and bound water makes these methods attractive to investigate corrosion [18,133], changes in hydration in nanoporous systems [1] or transitions of thermo-responsive polymers [63,118]. Dipole responses in biomaterials include studies covering a broad range of applications [62,89,119] from molecules [11,34,59,130] via cells [84,101,103,132] to tissues [27,104]. In the framework of this book, two examples for application from the biosciences are being presented, both acquired in AC fields.…”

Section: Applicationsmentioning

confidence: 99%

“…Impedance measurements can be used to monitor cell behaviour in vitro [101] and in vivo [4,45,131]. Recently developed chip-based methods allow for spatial and temporal control of cell growth and cell stimuli.…”

Section: Lab-on-a-chip Systems In Generalmentioning

confidence: 99%

“…In such a case, it is sufficient to restrict the impedance measurements to one defined test frequency. This optimal frequency differs among different cell types and needs to be determined ahead of the experiments using cells attached onto the chip surface [101]. The test frequency is then determined from the maximal Fig.…”

Section: Impedance Spectroscopy With Ides In Lab-on-a-chip Devicesmentioning

confidence: 99%

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Bioimpedance Spectroscopy

Klösgen

Rümenapp

Gleich

2011

BetaSys

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In the context of biology, electrical phenomena are usually identified with ionic currents through protein channels, for example as they are postulated during nerve signalling (Andersen et al. Prog Neurobiol 88(2):104-113, 2009; Sakmann and Neher Annu Rev Physiol 46:455-472, 1984). However, there are more electrical phenomena that play a significant role in biological systems, namely those arising from polarization effects (Grimnes Bioimpedance and Bioelectricity, 2008; Polk Handbook of Biological Effects of Electromagnetic Fields 1996). Local inhomogeneities in charge distributions give rise to the formation of permanent molecular dipoles as in uncharged molecules such as (hydration) water, or due to the polar groups in proteins and lipid molecules. Non-permanent electrical dipoles can originate from the presence of ions in solution. Structured distributions of counter-ions at all polar interfaces, for example along the surface of proteins and especially along the polar membrane interfaces of cells, cause the formation of Stern and Helmholtz layers. All non-uniform distributions of charges and dipoles initiate and modify internal local electrical fields. Moreover, the application of external fields causes relaxation processes with characteristic contributions to the frequency-dependent complex dielectric constant. These dipolar relaxations were initially described by Debye (

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Section: Applicationsmentioning

confidence: 99%

Section: Lab-on-a-chip Systems In Generalmentioning

confidence: 99%

Section: Impedance Spectroscopy With Ides In Lab-on-a-chip Devicesmentioning

confidence: 99%

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Bioimpedance Spectroscopy

Klösgen

Rümenapp

Gleich

2011

BetaSys

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“…Haas et al, also demonstrated that the switching of rat aortic SMCs from a proliferative to a quiescent phenotype could be determined by reductions in total impedance measured across a 1kHz to 1MHz range [ 34 ]. Rümenapp et al, also reported distinct total impedance ratio variations across the same frequency range when investigating the effect of drug toxicity on murine fibroblasts [ 35 ]. We therefore hypothesised that impedance measurements in the high frequency kilohertz range would enable non-invasive characterisation between different vascular cell types as they proliferate to confluence.…”

Section: Introductionmentioning

confidence: 99%

Towards non-invasive characterisation of coronary stent re-endothelialisation – An in-vitro, electrical impedance study

2018

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The permanent implantation of a stent has become the most common method for ameliorating coronary artery narrowing arising from atherosclerosis. Following the procedure, optimal arterial wall healing is characterised by the complete regrowth of an Endothelial Cell monolayer over the exposed stent surface and surrounding tissue, thereby reducing the risk of thrombosis. However, excessive proliferation of Smooth Muscle Cells, within the artery wall can lead to unwanted renarrowing of the vessel lumen. Current imaging techniques are unable to adequately identify re-endothelialisation, and it has previously been reported that the stent itself could be used as an electrode in combination with electrical impedance spectroscopic techniques to monitor the post-stenting recovery phase. The utility of such a device will be determined by its ability to characterise between vascular cell types. Here we present in-vitro impedance spectroscopy measurements of pulmonary artery porcine Endothelial Cells, Human Umbilical Vein Endothelial Cells and coronary artery porcine Smooth Muscle Cells grown to confluence over platinum black electrodes in clinically relevant populations. These measurements were obtained, using a bespoke impedance spectroscopy system that autonomously performed impedance sweeps in the 1kHz to 100kHz frequency range. Analysis of the reactance component of impedance revealed distinct frequency dependent profiles for each cell type with post confluence reactance declines in Endothelial Cell populations that have not been previously reported. Such profiles provide a means of non-invasively characterising between the cell types and give an indication that impedance spectroscopic techniques may enable the non-invasive characterisation of the arterial response to stent placement.

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“…Most cell impedance sensors monitor the impedance at a fixed frequency chosen for maximum sensitivity. 17 Other methods measure impedance over a range of frequencies. 18 The simplest approach to measuring the frequency spectrum in a cell-based impedance sensor is to sweep the frequencies.…”

Section: Toxinmentioning

confidence: 99%

Nanofluidic structures for coupled sensing and remediation of toxins

Shaw

Contento

et al. 2014

SPIE Proceedings

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One foundational motivation for chemical sensing is that knowledge of the presence and level of a chemical agent informs decisions about treatment of the agent, for example by sequestration, separation or chemical conversion to a less harmful substance. Commonly the sensing and treatment steps are separate. However, the disjoint detection/treatment approach is neither optimal, nor required. Thus, we are investigating how nanostructured architectures can be constructed so that molecular transport (analyte/reagent delivery), chemical sensing (optical or electrochemical) and subsequent treatment can all be coupled in the same physical space during the same translocation event. Chemical sensors that are uniquely well-poised for integration into 3-D micro-/nanofluidic architectures include those based on plasmonics and impedance. Following detection, treatment can be substantially enhanced if mass transport limitations can be overcome. In this context, in situ generation of reactive species within confined geometries, such as nanopores or nanochannels, is of significant interest, because of its potential utility in overcoming mass transport limitations in chemical reactivity. Solvent electrolysis in electrochemically coupled nanochannels supporting electrokinetic flow for the generation of reactive species, can produce arbitrarily tunable quantities of reagents, such as O 2 or H 2 , in situ in close proximity to the site of a hydrogenation catalyst, for example. Semi-quantitative estimates of the local H 2 concentration are obtained by comparing the spatiotemporal fluorescence behavior and current measurements with finite element simulations accounting for electrolysis and subsequent convection and diffusion within the confined geometry. H 2 saturation can easily be achieved at modest overpotentials.

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Improved method for impedance measurements of mammalian cells

Cited by 24 publications

References 17 publications

Bioimpedance Spectroscopy

Bioimpedance Spectroscopy

Towards non-invasive characterisation of coronary stent re-endothelialisation – An in-vitro, electrical impedance study

Nanofluidic structures for coupled sensing and remediation of toxins

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