Improved method for bergapten determination by high-performance liquid chromatography

Shu, Chi-Kuen; Walradt, John P.; Taylor, Welton I.

doi:10.1016/s0021-9673(00)93835-4

Cited by 29 publications

(10 citation statements)

References 7 publications

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“…Apart from furocoumarins 1-15, angelicin (19), bergaptol (16) and isobergapten (20) should also be analysed because of their occurrence in Angelica archangelica, 55 Angelica genuflexa 56 lemon and bergamot oils 57 and Heracleum species. [58][59][60] Psora-4 (18) and 5-(4-phenoxybutoxy)psoralen (17) could be analysed for a specificity purpose even if not reported in nature.…”

Section: Elution Conditionsmentioning

confidence: 99%

“…To comply with regulations and low expected concentration limits, sensitive and specific analytical methods might be used. High‐performance liquid chromatography coupled with a diode array detector (HPLC‐DAD) is the method of choice generally reported for their analysis . In 2004, Frérot et al proposed a reversed phase HPLC method with UV detection at 310 nm to separate and quantify the 15 most common furocoumarins ( 1 – 15 ) in essential oils .…”

Section: Introductionmentioning

confidence: 99%

“…Highperformance liquid chromatography coupled with a diode array detector (HPLC-DAD) is the method of choice generally reported for their analysis. [15][16][17][18][19][20] In 2004, Frérot et al proposed a reversed phase HPLC method with UV detection at 310 nm to separate and quantify the 15 most common furocoumarins (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) in essential oils. 18 This method was validated in a collaborative ring test by the IFRA Analytical Working Group 8 years later.…”

mentioning

confidence: 99%

“…32,41 Recently, HPTLC with a scanning system has stimulated a growing interest for the routine determination of target compounds. 46 Thus, the aim of this study was (i) to propose a new HPTLC method to analyse the 20 most common furocoumarins (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) found in perfumery raw materials for screening purposes ( Figure 1). and (ii) to propose a validated HPTLC method allowing the quantification of bergapten in essential oils in accordance with regulated limits.…”

mentioning

confidence: 99%

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HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

Borgne¹,

Cicchetti²,

Bertrand³

2017

Flavour & Fragrance J

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The concentration of furocoumarins, known to induce mutagenesis, carcinogenesis and photodermatitis under UV radiation, is limited by the EU cosmetic regulation in consumer products such as cosmetics, sun protection and bronzing formulations. The bergapten concentration is also limited by the International Fragrance Association. Analytical methods have been proposed for their quantification using high‐performance liquid chromatography (HPLC) coupled with UV detection or mass spectrometry with limits of detection (LOD) and quantification (LOQ) in accordance with the regulation. These methods are time and solvent consuming for routine quality control analyses. In this context, simple and fast HPLC methods in one‐ (1‐D) and two‐ (2‐D) dimensions were developed for the simultaneous analysis of the 15 most common furocoumarins reported in Citrus essential oils: psoralen, bergapten, xanthotoxin, isopimpinellin, oxypeucedanin, oxypeucedanin hydrate, byakangelicol, byakangelicin, heraclenin, 8‐geranyloxypsoralen, bergamottin, imperatorin, isoimperatorin, phellopterin, epoxybergamottin along with five other furocoumarins known to be present in Angelica, bergamot or Heracleum species: angelicin, bergaptol, isobergapten, psora‐4 and 5‐(4‐phenoxybutoxy)psoralen. The 1‐D method was validated for the quantification of bergapten using a Camag TLC Scanner as a detector. The LOD was determined at 2 mg/kg. The method was linear from 2 to 100 mg/kg and the method range was defined using accuracy profiles at with an 80% tolerance interval, with a 30% error, to be 5–100 mg/kg in acetonitrile and 13–100 mg/kg in matrix.

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Section: Elution Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

Borgne¹,

Cicchetti²,

Bertrand³

2017

Flavour & Fragrance J

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“…At present, furocoumarins are essentially quantified using high pressure liquid chromatography (HPLC) techniques (Shu et al, 1975;Vande Casteele and Van Sumere, 1983;Thompson and Brown, 1984). However, furocoumarins are strongly phototoxic when associated Author to whom correspondence should be ;iddrcssed.…”

Section: Introductionmentioning

confidence: 99%

A selective photobiological assay to detect and quantify psoralen in Psoralea plants (Leguminosae)

Bourgaud

Grimal

Nguyen

et al. 1994

Phytochemical Analysis

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A simple and rapid bioassay involving the gram‐positive bacterium Bacillus brevis was developed to detect and quantify furocoumarins (psoralen and angelicin) in Psoralea plants (Leguminosae). Small paper discs, soaked with furocoumarin standards or the corresponding plant samples, are placed on B. brevis lawns in Petri dishes and irradiated for 24 h with UVA. The diameter of the growth inhibition zone around the discs is very well‐correlated with the quantity of furocoumarin standard in the disc. Because psoralen is more phototoxic than angelicin, its detection limit (5 × 10−8 g/disc) was lower than angelicin (5 × 10−7 g/disc). Consequently, it is possible to dilute a crude plant extract in order to reach a detectable psoralen concentration whilst maintaining the angelicin concentration below its lower detection limit. In this condition, angelicin does not interfer with psoralen detection, and the growth inhibition is entirely due to psoralen. The bioassay was validated using a classical high pressure liquid chromatographic method and the correlation between the two techniques was satisfactory with plant samples. Therefore, the bioassay can be considered as a sensitive, simple, rapid and selective method to quantify psoralen in Psoralea plants.

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Acetylenes and Psoralens

Christensen¹,

Brandt²

2006

Plant Secondary Metabolites

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Improved method for bergapten determination by high-performance liquid chromatography

Cited by 29 publications

References 7 publications

HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

A selective photobiological assay to detect and quantify psoralen in Psoralea plants (Leguminosae)

Acetylenes and Psoralens

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