Improved Medium for Selecting Nitrate-Nonutilizing (<i>nit</i>) Mutants of <i>Verticillium dahliae</i>

Korolev, Nadia; Katan, Talma

doi:10.1094/phyto.1997.87.10.1067

Cited by 59 publications

(44 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At least four nitrate nonutilizing (nit) mutants from each of 67 pepper and four tomato isolates of V. dahliae were generated using techniques described previously (12,19). Complementation tests for each nit mutant were done by pairing the mutant isolate with standard NitM tester strains of V. dahliae belonging to VCG 1 (strain T9), VCG 2 (strain 115), VCG 3 (strain PCW), and VCG 4 (strain S39).…”

Section: Methodsmentioning

confidence: 99%

Characterization of Verticillium dahliae Isolates and Wilt Epidemics of Pepper

Bhat

Smith²,

Koike³

et al. 2003

Plant Disease

104

View full text Add to dashboard Cite

Bhat, R. G., Smith, R. F., Koike, S. T., Wu, B. M., and Subbarao, K. V. 2003. Characterization of Verticillium dahliae isolates and wilt epidemics of pepper. Plant Dis. 87:789-797.Epidemics of Verticillium wilt in pepper fields of the central coast of California and isolates of Verticillium dahliae associated with these epidemics were characterized. The mean incidence of wilted plants per field ranged from 6.3 to 97.8% in fields with Anaheim, jalapeno, paprika, or bell peppers. In general, incidence of wilt in jalapeno and bell pepper crops was lower than in crops of other types of pepper. Inoculum density of V. dahliae in the surveyed pepper fields ranged from 2.7 to 66.6 microsclerotia g -1 dry soil, and the correlation between disease incidence and density of microsclerotia was high (r = 0.81, P < 0.01). Distribution of Verticillium wilt was aggregated in a majority of the pepper fields surveyed, but the degree of aggregation varied. Vegetative compatibility group (VCG) characterization of 67 isolates of V. dahliae indicated that 67% belonged to VCG 2, 22% to VCG 4, and 11% to a new group, designated VCG 6. The pathogenicity of isolates of V. dahliae from bell pepper and tomato plants was tested by inoculating 1-month-old bell pepper (cv. Cal Wonder) and tomato (cv. EP 7) seedlings and incubating the inoculated plants in the greenhouse. Seedlings of bell pepper were susceptible only to the isolates of V. dahliae from pepper, whereas seedlings of tomato were susceptible to both pepper and tomato isolates. Pepper isolates belonging to VCG 2, VCG 4, and VCG 6 were highly pathogenic to bell pepper and chili pepper. Temperatures between 15 and 25°C were optimal for mycelial growth of a majority of isolates of V. dahliae. Molecular characterization of pepper isolates of V. dahliae using a polymerase chain reaction (PCR)-based random amplified polymorphic DNA (RAPD) technique revealed minor variation among these isolates, but unique polymorphic banding patterns were observed for isolates belonging to VCG 6. Verticillium wilt of pepper is a major production constraint in the central coast of California. More aggressive isolates of V. dahliae may have been selected in this region as a result of intensive cropping practices.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of Verticillium dahliae Isolates and Wilt Epidemics of Pepper

Bhat

Smith²,

Koike³

et al. 2003

Plant Disease

104

View full text Add to dashboard Cite

show abstract

“…Verticillium dahliae VCGs are mostly identified using spontaneous nitrate-nonutilizing (nit) mutants. If two isolates belong to the same VCG, their nit mutants will anastomose and produce wild-type growth in complementation assays (Korolev and Katan 1997). Isolates belonging to a VCG have the potential to share the same "pool" of genes and would be genetically separated from other individuals belonging to a different VCG thanks to socalled incompatibility mechanisms (Leslie 1993).…”

Section: Pathogenicity Groupsmentioning

confidence: 99%

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

2010

View full text Add to dashboard Cite

Olive (Olea europaea L.) is one of the first domesticated and cultivated tree species and has historical, social and economical relevance. However, its future as a strategic commodity in Mediterranean agriculture is threatened by diverse biotic (traditional and new/emerging pests and diseases) and abiotic (erosion, climate change) menaces. These problems could also be of relevance for new geographical areas where olive cultivation is not traditional but is increasingly spreading (i.e., South America, Australia, etc). One of the major constraints for olive cultivation is Verticillium wilt, a vascular disease caused by the soil-borne fungus Verticillium dahliae Kleb. In this review we describe how Verticillium wilt of olive (VWO) has become a major problem for olive cultivation during the last two decades. Similar to other vascular diseases, VWO is difficult to manage and single control measure are mostly ineffective. Therefore, an integrated disease management strategy that fits modern sustainable agriculture criteria must be implemented. Multidisciplinary research efforts and advances to understand this pathosystem and to develop appropriate control measures are summarized. The main conclusion is that a holistic approach is the best strategy to effectively control VWO, integrating biological, chemical, physical, and cultural approaches.

show abstract

“…Nit mutants of the isolates were generated on water agar chlorate (WAC) medium and identified on Czapek Dox agar (CDA) according to Korolev and Katan (1997). Colonies presenting a faint growth on CDA with no aerial mycelium were labelled as nit mutants and phenotyped on CDA amended with hypoxanthine as described by Correll et al (1987).…”

Section: Genetic Characterizationmentioning

confidence: 99%

Genetic and Molecular Approach to Verticillium dahliae Infecting Sunflower

García-Carneros

García-Ruiz

Molinero-Ruiz

2014

Helia

View full text Add to dashboard Cite

Verticillium leaf mottle and wilt caused by the fungus Verticillium dahliae is a major disease of sunflower in Argentina and the USA. In the summer of 2013, an important outbreak of the disease occurred in one field in the Southwest of Spain. The determination of vegetative compatibility groups (VCGs) of V. dahliae is used in the genetic characterization of the fungus. Molecular markers have also been developed and applied for the analysis of V. dahliae, particularly for those isolates infecting artichoke, cotton and olive tree. The objective of this work was to determine the genetic and molecular features of V. dahliae of sunflower. Three V. dahliae isolates, one from Argentina collected in 2012 and two collected in Spain in 2013, were analysed. The VCGs were determined by complementation between nit mutants of the isolates from sunflower and the VCGs reference strains. Molecular characterization was conducted by polymerase chain reaction using primer pairs that are diagnostic of either V. dahliae species, defoliating or non-defoliating pathotypes, or VCGs. Complementation tests between nit mutants and reference strains clearly showed that the three isolates from sunflower belong to the VCG2B. The VCG2B has been identified in V. dahliae from crops as cotton, artichoke, eggplant, pepper and tomato among others. When molecularly analysed, the three V. dahliae isolates infecting sunflower had the same molecular pattern than the one found for non-defoliating isolates of V. dahliae pathogenic to artichoke or cotton. The results of this work show the closeness between non-defoliating isolates of V. dahliae infecting artichoke, cotton and sunflower and suggest that any of these three species can serve as carrier and source of inoculum for Verticillium outbreaks in them.

show abstract

Improved Medium for Selecting Nitrate-Nonutilizing (nit) Mutants of Verticillium dahliae

Cited by 59 publications

References 23 publications

Characterization of Verticillium dahliae Isolates and Wilt Epidemics of Pepper

Characterization of Verticillium dahliae Isolates and Wilt Epidemics of Pepper

Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen

Genetic and Molecular Approach to Verticillium dahliae Infecting Sunflower

Contact Info

Product

Resources

About