Improved matrix coating for positive- and negative-ion-mode MALDI-TOF imaging of lipids in blood vessel tissues

Meisenbichler, Christina; Doppler, Christian; Bernhard, David; Müller, Thomas

doi:10.1007/s00216-019-01826-x

Cited by 17 publications

(16 citation statements)

References 38 publications

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“…Matrix recrystallization was performed according to Meisenbichler et al [24] with some modifications. One mL of 5% aqueous methanol containing 10 mM acetic acid was placed in a glass Petri dish (90 mm in diameter), and preheated at 37 • C on the hot plate for 10 min.…”

Section: Matrix Coatingmentioning

confidence: 99%

“…In MALDI-MSI, the fabrication of a homogeneous analyte-containing matrix coating onto the sample surface is a prerequisite for both high sensitivity and high spatial resolution [24,25]. Spraying a matrix in solution is commonly used for matrix coating; however, this involves the risk of lateral analyte delocalization.…”

Section: Introductionmentioning

confidence: 99%

“…Spraying a matrix in solution is commonly used for matrix coating; however, this involves the risk of lateral analyte delocalization. On the other hand, in recent years, matrix vapor deposition/recrystallization has received much attention for its potential to overcome these problems [24]. Thus, for example, phospholipids in different cell layers of blood vessel walls were successfully visualized with high quality, using DAN by this matrix coating method for MALDI-MSI analysis [24].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, in recent years, matrix vapor deposition/recrystallization has received much attention for its potential to overcome these problems [24]. Thus, for example, phospholipids in different cell layers of blood vessel walls were successfully visualized with high quality, using DAN by this matrix coating method for MALDI-MSI analysis [24]. In contrast, the application of matrix vapor deposition/recrystallization for analysis of plant tissues has been limited.…”

Section: Introductionmentioning

confidence: 99%

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Localization of Flavan-3-ol Species in Peanut Testa by Mass Spectrometry Imaging

Enomoto

Nirasawa²

2020

Molecules

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Flavan-3-ols, procyanidins and their monomers are major flavonoids present in peanuts that show a wide range of biological properties and health benefits, based on their potent antioxidant activity. Procyanidin oligomers, especially A-type, are reportedly abundant in peanut skin; however, their localization in the raw peanut testa remains poorly understood. Therefore, we performed matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to investigate the localization of flavan-3-ols in peanut testa. 1,5-Diaminonaphthalene was coated onto the peanut section by matrix vapor deposition/recrystallization, and MALDI-MSI measurements were performed in the negative-ion mode. Peaks matching the m/z values of flavan-3-ol [M − H]− ions were observed in the mass spectrum extracted from the outer epidermis of the peanut testa, using the region of interest function. Catechin and/or epicatechin, five A-type, and one B-type procyanidins were assigned by the fragment ions generated by retro-Diels-Alder, heterocyclic ring fission, and quinone methide reactions detected in MALDI-tandem MS spectra. These flavan-3-ols were localized in the outer epidermis of the peanut testa. This information will contribute to improving the extraction and purification efficiencies of flavan-3-ols from peanut testa. As flavan-3-ols display anti-microbial activity, it is speculated that flavan-3-ols present in the outer epidermis of peanut testa act to prevent pathogen infection.

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Section: Matrix Coatingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Localization of Flavan-3-ol Species in Peanut Testa by Mass Spectrometry Imaging

Enomoto

Nirasawa²

2020

Molecules

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“…However, owing to its solvent-free mechanism, a recrystallisation step after sublimation can be necessary to allow better extraction of analyte of interest from the sample and therefore a more intense signal (Yang and Caprioli, 2011). Commonly the recrystallisation step is performed by incubation with solvent vapour (Yang and Caprioli, 2011;Meisenbichler et al, 2019;Morikawa-Ichinose et al, 2019), although the literature has also reported the use of sprayers for solvent application (Ferguson et al, 2013;Lauzon et al, 2015; Dueñas, Carlucci and Lee, 2016).…”

Section: Introductionmentioning

confidence: 99%

A Quantitative MALDI-MSI Study of the Movement of Molecules in Biological Systems

Russo¹

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Mass spectrometry imaging to detect lipid biomarkers and disease signatures in cancer

2019

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BackgroundCurrent methods to identify, classify, and predict tumor behavior mostly rely on histology, immunohistochemistry, and molecular determinants. However, better predictive markers are required for tumor diagnosis and evaluation. Due, in part, to recent technological advancements, metabolomics and lipid biomarkers have become a promising area in cancer research. Therefore, there is a necessity for novel and complementary techniques to identify and visualize these molecular markers within tumors and surrounding tissue.Recent FindingsSince its introduction, mass spectrometry imaging (MSI) has proven to be a powerful tool for mapping analytes in biological tissues. By adding the label‐free specificity of mass spectrometry to the detailed spatial information of traditional histology, hundreds of lipids can be imaged simultaneously within a tumor. MSI provides highly detailed lipid maps for comparing intra‐tumor, tumor margin, and healthy regions to identify biomarkers, patterns of disease, and potential therapeutic targets. In this manuscript, recent advancement in sample preparation and MSI technologies are discussed with special emphasis on cancer lipid research to identify tumor biomarkers.ConclusionMSI offers a unique approach for biomolecular characterization of tumor tissues and provides valuable complementary information to histology for lipid biomarker discovery and tumor classification in clinical and research cancer applications.

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Improved matrix coating for positive- and negative-ion-mode MALDI-TOF imaging of lipids in blood vessel tissues

Cited by 17 publications

References 38 publications

Localization of Flavan-3-ol Species in Peanut Testa by Mass Spectrometry Imaging

Localization of Flavan-3-ol Species in Peanut Testa by Mass Spectrometry Imaging

A Quantitative MALDI-MSI Study of the Movement of Molecules in Biological Systems

Mass spectrometry imaging to detect lipid biomarkers and disease signatures in cancer

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