Improved Mass Multiplication of Rhodiola crenulata Shoots Using Temporary Immersion Bioreactor with Forced Ventilation

Abstract

A temporary immersion bioreactor system was found to be suitable for mass shoot proliferation of Rhodiola crenulata. The shoot multiplication ratio and hyperhydration rate reached 46.8 and 35.4%, respectively, at a temporary immersion cycle of 3-min immersion every 300 min. Forced ventilation was employed in the temporary immersion bioreactor culture in order to decrease the hyperhydration rate, improve shoot quality and enhance the multiplication ratio. The highest multiplication ratio of 55.7 was obtained under a temporary immersion cycle of 3-min immersion every 180 min with the forced ventilation at an air flow rate of 40 l/h, and the hyperhydration rate was reduced to 26.1%. Forced ventilation also improved the subsequent elongation and rooting rate of these proliferated shoots, and the shoot cultures from the temporary immersion bioreactor formed complete plantlets when subcultured onto a rooting medium containing 5 μmol/l indole-3-acetic acid.

Cattleya walkeriana growth in different micropropagation systems

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Engineering Salidroside Biosynthetic Pathway in Hairy Root Cultures of Rhodiola crenulata Based on Metabolic Characterization of Tyrosine Decarboxylase

“…R . crenulata belongs to the family of Crassulaceae and mostly grows in the very harsh environments with high altitude of 5000 meters and above, low oxygen concentration, strong ultraviolet radiation and poor soils [2]. So, R .…”

Effects ofin vitroculture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipiferaL.) from somatic embryos

“…Because the TIB type supplies sufficient oxygen, it appears to offer advantages in the reduction of hyperhydricity and increase in stimulatory growth substances (Alister et al 2005;Zhao et al 2012). Plant tissue culture techniques have been employed to propagate many species but acclimation is still a problem for successful plant production (Preece and Sutter 1991;Kozai 1999).…”