Improved low-invasive mRNA electroporation method into immature mouse oocytes visualizes protein dynamics during development
Yuhkoh Satouh,
Emiko Suzuki,
Keisuke Sasaki
et al.
Abstract:One of the major causes of oocyte quality deterioration, chromosome segregation abnormalities manifest mainly during meiosis I, which occurs before and during ovulation. However, currently, there is a technical limitation in the introduction of mRNA into premature oocytes without impairing embryonic developmental ability. In this study, we established a low-invasive electroporation (EP) method to introduce mRNA into pre-ovulatory, germinal vesicle (GV) mouse oocytes in an easier manner than the traditional mic… Show more
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