Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding

Li, Kun-tai; Liu, Donghong; Li, Yongliang; Chu, Ju; Wang, Yonghong; Zhuang, Yingping; Zhang, Siliang

doi:10.1016/j.biortech.2008.03.023

Cited by 43 publications

(27 citation statements)

References 20 publications

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“…To elucidate the mechanism of high-productivity vitamin B 12 under fermentation using a DO step-wise reduction strategy, the metabolic kinetics of P. denitrificans was investigated in detail in a 5 L fermenter. Compared with P. denitrificans fermentation under the control strategies DO-stat and DO step-wise increase, it was found that a DO step-wise reduction strategy could not only result in accelerated EMP flux, but also significantly improve the activities of ALA synthase and PBG synthase, so that more precursors for vitamin B 12 biosynthesis could be generated.…”

Section: Discussionmentioning

confidence: 99%

“…20 Therefore, the generation of these amino acids was crucial to the production of vitamin B 12 . As an important intermediate in the TCA, α-ketoglutarate plays an important role in the regulation of the balance between carbon and nitrogen metabolism in most microorganisms, which is the metabolic precursor for glutamate generation.…”

Section: Activities Kinetics Of the Key Enzymes Involved In The Centrmentioning

confidence: 99%

“…For example, Wang et al 13 demonstrated that high oxygen transfer rates could accelerate cell growth and initial vitamin B 12 biosynthesis rate of P. denitrificans, while the limiting DO level was critical for higher productivity in the late fermentation process. Similarly, Li et al 10 pointed out that the comparatively low DO level was favorable for vitamin B 12 biosynthesis, but it would have an extremely negative effect on cell growth.…”

Section: Introductionmentioning

confidence: 97%

“…Vitamin B 12 belongs to the family of cobalt corrinoid, which is an important biologically active compound widely used for the treatment of pernicious anemia and peripheral neuritis. 1,2 Because the chemical synthesis processes are highly complicated and costly, some industrial microorganisms have been successfully developed for the commercial production of vitamin B 12 , such as Pseudomonas denitrificans, Propionibacterium freudenreichii, and Propionibacterium shermanii.…”

Section: Introductionmentioning

confidence: 99%

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The metabolic characteristicsof high‐production vitamin B₁₂ by Pseudomonas denitrificans under dissolved oxygen step‐wise reduction

Peng

Cheng

Zhang

et al. 2013

J of Chemical Tech & Biotech

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BACKGROUND: Previous research proved that fermentation via dissolved oxygen (DO) step-wise reduction was favorable for vitamin B 12 productivity by Pseudomonas denitrificans, but the high-production mechanism is still unclear. Based on this fact, P. denitrificans metabolic characteristics under different DO supply models were investigated in a 5 L fermenter by assaying the activities of the key enzymes involved in central carbon metabolism and the vitamin B 12 biosynthetic pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Activities Kinetics Of the Key Enzymes Involved In The Centrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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The metabolic characteristicsof high‐production vitamin B₁₂ by Pseudomonas denitrificans under dissolved oxygen step‐wise reduction

Peng

Cheng

Zhang

et al. 2013

J of Chemical Tech & Biotech

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“…The microorganisms most widely used in these fermentations include Propionibacterium spp. [13,27], Pseudomonas denitrificans [21], methanogens (Methanosarcina barkeri, Methanobacterium spp.) and Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii

Wyk

Britz

2010

Dairy Sci. Technol.

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-To overcome nutritional vitamin B 12 deficiencies in certain populations in Southern Africa, fortified functional foods have been developed. However, current microbiological methods used to accurately determine vitamin B 12 levels in foodstuffs, important for quality control and regulatory purposes, are time consuming. This study describes an extraction and detection method for vitamin B 12 in dairy products and growth media cultured with Propionibacterium freudenreichii. Samples were extracted by mixing in KCN buffer (pH 4.5), autoclaving at 121°C for 25 min, cooling and centrifugation. The resultant supernatant was syringe-filtered prior to reversed-phase HPLC analysis using a methanol-water gradient that was effective in resolving the B 12 peak. The method offered excellent linearity with a regression coefficient R > 0.998. The limit of quantification was 0.005 μg·mL −1 sample. For samples with vitamin B 12 concentrations well within the linear range of the assay, good repeatability was demonstrated for the same sample with mean concentrations of 2.62 ± 0.02 and 2.61 ± 0.02 μg·mL −1 detected on day 1 and day 2, respectively. Recovery values ranged from 98.6% to 103.2%, indicating that the extraction method ensured complete dissolution of vitamin B 12 from the matrices under study. Sensitivity was enhanced by sample concentration and purification using a series of solid phase extraction steps which resulted in improved peak resolution and removal of interfering peaks. The method was validated by comparison of the HPLC results of the same sample with those obtained using traditional microbiological methods. The method is a rapid alternative to the more time-consuming microbiological assay. Résumé -Une méthode HPLC rapide pour extraire et quantifier la vitamine B 12 dans les produits laitiers et les cultures de Propionibacterium freudenreichii. Pour lutter contre les carences en vitamine B 12 parmi certaines populations d'Afrique du Sud, des aliments fonctionnels enrichis ont été développés. Cependant, les méthodes actuelles utilisées pour analyser précisément le niveau de vitamine B 12 dans les aliments, qui sont importantes à des fins de contrôle-qualité et de législation, sont coûteuses en temps. Cet article décrit une méthode d'extraction et de détection de la vitamine B 12 dans les produits laitiers et les cultures de Propionibacterium freudenreichii. Les échantillons étaient extraits par mélange à du tampon KCN, pH 4,5 et autoclavage à 121°C pendant 25 min, refroidissement et centrifugation. Le surnageant résultant était filtré puis analysé par HPLC en phase inverse en utilisant un gradient méthanol-eau, qui permettait une bonne résolution du pic de vitamine B 12 . La méthode montrait une bonne linéarité, avec un coefficient de régression R > 0,998. La limite de quantification était de 0,005 μg·mL −1 d'échantillon. Pour des échantillons contenant des concentrations dans la moyenne de la gamme de linéarité, la répétabilité a été établie, avec des valeurs obtenues pour le même échantillon à deux jo...

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Synthetic Biology in Metabolic Engineering: From Complex Biochemical Pathways to Compartmentalized Metabolic Processes - a Vitamin Connection

Deery

Frank

Lawrence

et al. 2014

Encyclopedia of Molecular Cell Biology and Molecular Medicine

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Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding

Cited by 43 publications

References 20 publications

The metabolic characteristicsof high‐production vitamin B₁₂ by Pseudomonas denitrificans under dissolved oxygen step‐wise reduction

The metabolic characteristicsof high‐production vitamin B₁₂ by Pseudomonas denitrificans under dissolved oxygen step‐wise reduction

A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii

Synthetic Biology in Metabolic Engineering: From Complex Biochemical Pathways to Compartmentalized Metabolic Processes - a Vitamin Connection

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Improved large-scale production of vitamin B12 by Pseudomonas denitrificans with betaine feeding

Cited by 43 publications

References 20 publications

The metabolic characteristicsof high‐production vitamin B12 by Pseudomonas denitrificans under dissolved oxygen step‐wise reduction

The metabolic characteristicsof high‐production vitamin B12 by Pseudomonas denitrificans under dissolved oxygen step‐wise reduction

A rapid HPLC method for the extraction and quantification of vitamin B12in dairy products and cultures ofPropionibacterium freudenreichii

Synthetic Biology in Metabolic Engineering: From Complex Biochemical Pathways to Compartmentalized Metabolic Processes - a Vitamin Connection

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The metabolic characteristicsof high‐production vitamin B₁₂ by Pseudomonas denitrificans under dissolved oxygen step‐wise reduction

The metabolic characteristicsof high‐production vitamin B₁₂ by Pseudomonas denitrificans under dissolved oxygen step‐wise reduction

A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii