Improved in vitro endothelialization on nanostructured titania with tannin/glycosaminoglycan-based polyelectrolyte multilayers

Sabino, Roberta M.; Kipper, Matt J.; Martins, Alessandro F.; Popat, Ketul C.

doi:10.1007/s44164-022-00024-x

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…During the fabrication of TiO 2 NTs, a decrease in intensity was caused by the decrease of the Ti/TiO 2 NT ratio in the samples which in turn was due to fabricated NTs being in the amorphous form without any heat treatment. , MTT assay control presents that the end-product TiO 2 NT-based membranes have a cell viability of 93.06%. They are biocompatible to be used in biofiltration applications and miniaturized blood purification devices. − Normalized MTT test results of control groups and samples are given in Figure d.…”

Section: Results and Discussionmentioning

confidence: 99%

Electrostatic Repulsive Features of Free-Standing Titanium Dioxide Nanotube-Based Membranes in Biofiltration Applications

2023

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This study presents the electrostatic repulsive features of electrochemically fabricated titanium dioxide nanotube (NT)-based membranes with different surface nanomorphologies in cross-flow biofiltration applications while maintaining a creatinine clearance above 90%. Although membranes exhibit antifouling behavior, their blood protein rejection can still be improved. Due to the electrostatically negative charge of the hexafluorotitanate moiety, the fabricated biocompatible, superhydrophilic, free-standing, and amorphous ceramic nanomembranes showed that about 20% of negatively charged 66 kDa blood albumin was rejected by the membrane with ∼100 nm pores. As the nanomorphology of the membrane was shifted from NTs to nanowires by varying fabrication parameters, pure water flux and bovine serum albumin (BSA) rejection performance were reduced, and the membrane did not lose its antifouling behavior. Herein, nanomembranes with different surface nanomorphologies were fabricated by a multi-step anodic oxidation process and characterized by scanning electron microscopy, atomic force microscopy, water contact angle analysis, X-ray diffraction, and energy-dispersive X-ray spectroscopy. The membrane performance of samples was measured in 3D printed polyethylene terephthalate glycol flow cells replicating implantable artificial kidney models to determine their blood toxin removal and protein loss features. In collected urine mimicking samples, creatinine clearances and BSA rejections were measured by the spectrophotometric Jaffe method and high-performance liquid chromatography.

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