Improved in vitro embryo development using in vivo matured oocytes from heifers superovulated with a controlled preovulatory lh surge

Leemput, E.E. van de; Vos, P.L.A.M.; Zeinstra, E.; Severs, Mirjam; Weijden, G.C. van der; Dieleman, S.J.

doi:10.1016/s0093-691x(99)00133-8

Cited by 110 publications

(62 citation statements)

References 34 publications

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“…The rate of blastocyst development in the post-LH group differed from recent findings in which exceptionally high rates of blastocyst development were found (Van de Leemput et al, 1999;Hendriksen et al, 2000). When two cows with low rates of blastocyst development were excluded from analysis, the rate of blastocyst development of the post-LH group increased to approximately 45% and the relative abundance of the genes did not change significantly.…”

Section: Discussioncontrasting

confidence: 84%

“…In contrast, for production of viable embryos in vitro, oocytes are used that differ from oocytes originating from ovulatory follicles in vivo in that they are: (1) derived from immature, subordinate follicles of 2-8 mm in diameter, and (2) matured in vitro, outside the follicle. Oocytes from small follicles (< 2-3 mm) are less likely to develop in vitro into blastocysts than are oocytes from larger (> 3 mm) follicles (Pavlok et al, 1992;Blondin et al, 1997;Van de Leemput et al, 1999). A comparative analysis of the ultrastructure of oocytes derived from dominant, preovulatory follicles with that of oocytes from subordinate, small follicles revealed first signs of maturation (referred to as 'capacitation' or 'prematuration') in the dominant follicle before the LH surge (Assey et al, 1994;Hyttel et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Effects of oocyte maturation regimen on the relative abundance of gene transcripts in bovine blastocysts derived in vitro or in vivo

Knijn

Wrenzycki²,

Hendriksen³

et al. 2002

Reproduction

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Bovine embryos produced in vitro differ substantially from embryos produced in vivo in the mRNA expression patterns of genes important for development. Several factors in the in vitro production systems have profound effects on embryonic mRNA expression patterns. The effects of the type of maturation on the expression pattern of genes important for development in blastocysts produced in vitro have not yet been investigated. The aim of the present study was to investigate the effects of various maturational protocols on the relative abundance of a panel of six marker genes, indicative of compaction and cavitation, metabolism, stress susceptibility and RNA processing, in bovine blastocysts produced in vitro. Four groups of blastocysts were analysed by a sensitive semi-quantitative RT-PCR assay. Blastocysts were produced in vitro from oocytes of different origin from: (1) 3-8 mm follicles; (2) preovulatory follicles before the LH surge; and (3) preovulatory follicles 24 h after the LH surge. The first two groups were matured in vitro, whereas the third group had undergone maturation in vivo. A fourth group comprised blastocysts developed entirely in vivo. Expression of glucose transporter 1 was significantly (P < 0.05) higher, and expression of desmocollin 2 and plakophilin tended to be higher (P < 0.1) for in vivo (group 4) compared with in vitro blastocysts (group 1), whereas no differences were found for heat shock protein 70.1, E-cadherin and poly(A) polymerase. Expression of the six transcripts did not differ among blastocysts produced in vitro from oocytes of groups 1, 2 and 3. Results indicate that alterations in the relative abundance of these transcripts in blastocysts produced in vitro cannot primarily be attributed to the origin of the oocyte, but are likely to have been induced by post-maturation or fertilization culture conditions.

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Section: Discussioncontrasting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Effects of oocyte maturation regimen on the relative abundance of gene transcripts in bovine blastocysts derived in vitro or in vivo

Knijn

Wrenzycki²,

Hendriksen³

et al. 2002

Reproduction

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“…This in agreement with several previous studies [11,26,45,52,80]. The data also support the notion that oocytes derived from large follicles are more competent than those derived from small follicles following IVP [46,61].…”

Section: Differences Between In Vivo-and In Vitro-produced Embryossupporting

confidence: 93%

Factors influencing oocyte and embryo quality in cattle

et al. 2001

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“…However, current IVM systems deliver suboptimal outcomes in terms of subsequent developmental competence (Leibfried-Rutledge et al 1987;van de Leemput et al 1999;Rizos et al 2002), limiting the use of this tool clinically ). Data collected from mouse models within our own laboratory demonstrate that, compared with in vivomatured (IVV) oocytes, IVM yields poorer embryo development, increased levels of apoptosis in resulting blastocysts and reduced implantation rates and fetal weights (Banwell et al 2007;Albuz et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Microarray analysis of mRNA from cumulus cells following in vivo or in vitro maturation of mouse cumulus–oocyte complexes

Kind

Banwell

Gebhardt

et al. 2013

Reprod. Fertil. Dev.

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The IVM of mammalian cumulus–oocyte complexes (COCs) yields reduced oocyte developmental competence compared with oocytes matured in vivo. Altered cumulus cell function during IVM is implicated as one cause for this difference. We have conducted a microarray analysis of cumulus cell mRNA following IVM or in vivo maturation (IVV). Mouse COCs were sourced from ovaries of 21-day-old CBAB6F1 mice 46 h after equine chorionic gonadotrophin (5 IU, i.p.) or from oviducts following treatment with 5 IU eCG (61 h) and 5 IU human chorionic gonadotrophin (13 h). IVM was performed in α-Minimal Essential Medium with 50 mIU FSH for 17 h. Three independent RNA samples were assessed using the Affymetrix Gene Chip Mouse Genome 430 2.0 array (Affymetrix, Santa Clara, CA, USA). In total, 1593 genes were differentially expressed, with 811 genes upregulated and 782 genes downregulated in IVM compared with IVV cumulus cells; selected genes were validated by real-time reverse transcription–polymerase chain reaction (RT-PCR). Surprisingly, haemoglobin α (Hba-a1) was highly expressed in IVV relative to IVM cumulus cells, which was verified by both RT-PCR and western blot analysis. Because haemoglobin regulates O2 and/or nitric oxide availability, we postulate that it may contribute to regulation of these gases during the ovulatory period in vivo. These data will provide a useful resource to determine differences in cumulus cell function that are possibly linked to oocyte competence.

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Improved in vitro embryo development using in vivo matured oocytes from heifers superovulated with a controlled preovulatory lh surge

Cited by 110 publications

References 34 publications

Effects of oocyte maturation regimen on the relative abundance of gene transcripts in bovine blastocysts derived in vitro or in vivo

Effects of oocyte maturation regimen on the relative abundance of gene transcripts in bovine blastocysts derived in vitro or in vivo

Factors influencing oocyte and embryo quality in cattle

Microarray analysis of mRNA from cumulus cells following in vivo or in vitro maturation of mouse cumulus–oocyte complexes

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