Improved in vitro Cultivation of Babesia caballi.

Abstract

ABSTRACT. Babesia caballi infected erythrocytes were collected from the blood of an experimentally infected horse and could be continuously cultivated in vitro with parasitemia ranging from 2-4% in RPMI 1640 medium supplemented with 2 mM L-glutamine, 20 mM HEPES and 40% adult horse serum in a low oxygen atmosphere (2% O 2 , 5% CO 2 and 93% N 2 ). All attempts to increase parasitemia failed using other culture media, serum concentrations and culture vessels. However, parasite growth was enhanced by transfer of cultures from a low oxygen to 5% CO 2 in air, with parasitemia ranging from 8-10%. -KEY WORDS: Babesia caballi, in vitro cultivation.

Age-dependent dynamics of Theileria equi and Babesia caballi infections in southwest Mongolia based on IFAT and/or PCR prevalence data from domestic horses and ticks

“…2 % and 84 . 5 % for T. equi and B. caballi, respectively, were observed (Avarzed et al 1997). In contrast to the present study, these authors found a significant decrease in prevalence in animals between 11 and 16 years, which may be due to the large stratification intervals (1, 2, 3, 4, 5 years, 6-10 years, 11-16 years) and the small sample size in this particular stratum (n=5).…”

Seroepidemiological Evidence for the Possible Presence of Babesia (Theileria) equi and Babesia caballi Infections in Donkeys in Western Xinjiang, China

“…Several reports on the prevalence of equine babesiosis in Mongolia, which shares a border with the Xinjiang, have been published [1,3,14]. These studies demonstrated that both B. equi and B. caballi infections in horses are widespread in Mongolia.…”

“…In the both ELISAs, the secondary goat anti-donkey IgG antibody (Rockland Immunochemicals, 1:4,000) was used. The IFAT using B. equi parasite or B. caballi parasite as antigens was performed as described previously [1], except that the secondary rabbit anti-donkey IgG antibody (Bethyl Laboratories, 1:400) was used.…”

Estimation of the transmission dynamics of Theileria equi and Babesia caballi in horses

“…Chronically infected horses represent a reservoir infecting ticks, which subsequently transmit the parasites to other equids. Piroplasms can be detected directly by microscopical examination of Giemsa-stained thin blood smears or by polymerase chain reaction (PCR) (Bruning, 1996), and for indirect diagnosis, the immunofluorescence antibody test (IFAT) is the most widely used technique (Gummow et al 1996 ;Avarzed et al 1997 ;Heuchert et al 1999).…”

“…Chronically infected horses represent a reservoir infecting ticks, which subsequently transmit the parasites to other equids. Piroplasms can be detected directly by microscopical examination of Giemsa-stained thin blood smears or by polymerase chain reaction (PCR) (Bruning, 1996), and for indirect diagnosis, the immunofluorescence antibody test (IFAT) is the most widely used technique (Gummow et al 1996 ;Avarzed et al 1997 ;Heuchert et al 1999).The epidemiology of equine piroplasmoses has been investigated in various studies (Mahoney, 1969 ;Mahoney and Ross, 1972 ;Ross and Mahoney, 1974 ;Smith, 1983 ;Dallwitz et al 1987 ;Medley et al 1993). In 2004, a study was conducted in a domestic horse population in south-western Mongolia (Rü egg et al 2007).…”