Improved implant osseointegration of a nanostructured titanium surface via mediation of macrophage polarization

Ma, Qianli; Zhao, Lei; Liu, Rongrong; Jin, Boquan; Song, Wen; Wang, Ying; Zhang, Yu-Si; Chen, Lihua; Zhang, Yumei

doi:10.1016/j.biomaterials.2014.08.025

Cited by 262 publications

(224 citation statements)

References 76 publications

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“…M2-like polarized macrophages are known to favor healing events following injury [26,27]. In our study, M2Ct-differentiated macrophages markedly enhanced fibroblast-mediated wound closure in vitro indicating favorable prohealing phenotype that is necessary for successful implant integration in vivo.…”

Section: Discussionsupporting

confidence: 54%

Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation

et al. 2017

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(2017) Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation. Acta Biomaterialia . ISSN 1878-7568 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/40797/1/2017%2001%2016_Ryabov_Acta %20Biomaterialia.pdf Copyright and reuse:The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. This article is made available under the University of Nottingham End User licence and may be reused according to the conditions of the licence. For more details see: http://eprints.nottingham.ac.uk/end_user_agreement.pdf A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.uk facilitated wound closure by human fibroblasts in co-culture conditions. Using a model for induction of inflammation by LPS we have shown that the M2Ct phenotype is stable for 12 days. However, in the absence of M2Ct in the medium macrophages underwent rapid pro-inflammatory re-programming upon IFNg stimulation. Therefore, loading and release of the cytokine cocktail from a self-standing, transferable Gelatin/Tyraminated Hyaluronic acid based release system was developed to stabilize macrophage phenotype for in vivo application in implantation and tissue engineering. The M2Ct cytokine cocktail retained its anti-inflammatory activity in controlled release conditions. Our data indicate that the direct application of a potent M2 inducing cytokine cocktail in a transferable release system can significantly improve the long term functionality of biomedical devices by decreasing proinflammatory cytokine secretion and increasing the rate of wound healing.

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Section: Discussionsupporting

confidence: 54%

Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation

et al. 2017

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“…[10][11][12] Although rapid physiological resolution of inflammation is conducive to tissue healing, continued chronic inflammation would result in the hyperplasia of fibrotic scar tissue, ultimately leading to implant failure. 13,14 Therefore, it would also be beneficial for early tissue healing and implant success to control the inflammatory process through macrophage polarization.…”

Section: Introductionmentioning

confidence: 99%

The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

Miao¹,

Wang²,

Xu³

et al. 2017

IJN

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Nanotopography modification is a major focus of interest in current titanium surface design; however, the influence of the nanostructured surface on human cell/bacterium behavior has rarely been systematically evaluated. In this study, a homogeneous nanofiber structure was prepared on a titanium surface (Nano) by alkali-hydrothermal treatment, and the effects of this Nano surface on the behaviors of human MG-63 osteoblasts, human gingival epithelial cells (HGECs) and human gingival fibroblasts (HGFs) were evaluated in comparison with a smooth titanium surface (Smooth) by polishing and a micro-rough titanium surface (Micro) by sandblasting and acid etching. In addition, the impacts of these different surface morphologies on human THP-1 macrophage polarization and Streptococcus mutans attachment were also assessed. Our findings showed that the nanostructured surface enhanced the osteogenic activity of MG-63 cells (Nano=Micro>Smooth) at the same time that it improved the attachment of HGECs (Nano>Smooth>Micro) and HGFs (Nano=Micro>Smooth). Furthermore, the surface with nanotexture did not affect macrophage polarization (Nano=Micro=Smooth), but did reduce initial bacterial adhesion (Nano

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“…Interestingly, recent observations in vivo show that 30 nm TiO 2 nanotubes were associated with less inflammatory cell infiltration (CD68 + macrophages) compared to 80 nm nanotubes. 35 An additional mechanism for the reduced inflammatory response can be related to the material surface adsorbed proteins and their conformational state as affected by the physicochemical properties of the nanotopography. Although this study did not explore the early (within hours) cell adhesion and protein content, it was evident, and not unexpected, that a protein matrix was observed on both surfaces at the earliest time point examined (3 days).…”

Section: Notesmentioning

confidence: 99%

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

Karazisis¹,

Ballo²,

Petronis³

et al. 2016

IJN

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Purpose Mechanisms governing the cellular interactions with well-defined nanotopography are not well described in vivo. This is partly due to the difficulty in isolating a particular effect of nanotopography from other surface properties. This study employed colloidal lithography for nanofabrication on titanium implants in combination with an in vivo sampling procedure and different analytical techniques. The aim was to elucidate the effect of well-defined nanotopography on the molecular, cellular, and structural events of osseointegration. Materials and methods Titanium implants were nanopatterned (Nano) with semispherical protrusions using colloidal lithography. Implants, with and without nanotopography, were implanted in rat tibia and retrieved after 3, 6, and 28 days. Retrieved implants were evaluated using quantitative polymerase chain reaction, histology, immunohistochemistry, and energy dispersive X-ray spectroscopy (EDS). Results Surface characterization showed that the nanotopography was well defined in terms of shape (semispherical), size (79±6 nm), and distribution (31±2 particles/µm 2 ). EDS showed similar levels of titanium, oxygen, and carbon for test and control implants, confirming similar chemistry. The molecular analysis of the retrieved implants revealed that the expression levels of the inflammatory cytokine, TNF-α , and the osteoclastic marker, CatK , were reduced in cells adherent to the Nano implants. This was consistent with the observation of less CD163-positive macrophages in the tissue surrounding the Nano implant. Furthermore, periostin immunostaining was frequently detected around the Nano implant, indicating higher osteogenic activity. This was supported by the EDS analysis of the retrieved implants showing higher content of calcium and phosphate on the Nano implants. Conclusion The results show that Nano implants elicit less periimplant macrophage infiltration and downregulate the early expression of inflammatory ( TNF -α) and osteoclastic ( CatK ) genes. Immunostaining and elemental analyses show higher osteogenic activity at the Nano implant. It is concluded that an implant with the present range of well-defined nanocues attenuates the inflammatory response while enhancing mineralization during osseointegration.

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Improved implant osseointegration of a nanostructured titanium surface via mediation of macrophage polarization

Cited by 262 publications

References 76 publications

Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation

Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation

The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

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Improved implant osseointegration of a nanostructured titanium surface via mediation of macrophage polarization

Cited by 262 publications

References 76 publications

Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation

Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation

The response of human osteoblasts, epithelial cells, fibroblasts, macrophages and oral bacteria to nanostructured titanium surfaces: a systematic study

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and&nbsp;molecular events in vivo

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The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo