Improved Salmonella recovery from moderate to highly polluted waters

Abstract

A new enrichment procedure for the recovery of salmonellas from aquatic environments is proposed. It has been tested in a eutrophic lake showing moderate to high faecal contamination levels (the Albufera lake near Valencia, Spain), and in effluents coming into a wastewater treatment plant. The new method consists of the addition of sodium novobiocin to a modification of Rappaport's medium (R10/43°C). The new medium (NR10/43°C) allows a better recovery of salmonellas from water than selenite broth.

The isolation of salmonellas and campylobacters

“…(1964 suggested that pre-enrichment may be detrimental to the isolation of salmonellas from heavily contaminated materials. Attempts have been made to use direct enrichment of polluted waters in selenite and Rappaport-Vassiliadis (RV) medium (Vassiliadis et al 1976a) with and without added novobiocin (Alcaide et al 1982(Alcaide et al , 1984. The first of these papers reported that the addition of novobiocin to both selenite and RV medium improved their selectivity when used for direct enrichment.…”

A comparison of the original Rappaport medium (R medium) and the Rappaport–Vassiliadis medium (RV medium) in the isolation of salmonellae from meat products

“…On the other hand, Alcaide et al (1982), using RV medium with the addition of novobiocin and van Schothorst & Renaud (1983), using RV medium made with soya peptone showed that the RV medium was significantly more efficient than selenite broth or Muller-Kauffmann tetrathionate broth (MK-ISO), respectively. Furthermore Fricker et al (1983), reported also that RV medium was significantly more efficient than Muller-Kauffmann tetrathionate and selenite F broths, in the isolation of salmonellae from pre-enriched seagull cloacal swabs.…”

Comparative study of different methods for detection and enumeration of Salmonella spp. in natural waters

“…Vassiliadis et al (1976,1978) modified the medium of Rappaport et al (1956) by decreasing the malachite green concentration, and raising the incubation temperature to 43°C. The addition of sodium novobiocin to selective media to improve recovery of salmonellas was proposed by several authors (Jeffries 1959;Hargrove et al 1971;Boothroyd & Baird-Parker 1973;Hoben et al 1973;Restaino et al 1977;Moats 1978;Alcaide et al 1982Alcaide et al , 1984.…”