Improved in vivo and in vitro viability of platelet concentrates stored for seven days in a platelet additive solution

Abstract

An additive solution has been developed for storage of platelet concentrates (PC) which sustains improved in vivo and in vitro viability after 7 d of storage in second generation oxygen permeable containers. This platelet additive solution is a protein-free physiologic salt solution fortified with citrate, bicarbonate and glucose. The in vivo quality of the PC was evaluated by autologous radiolabelling with Indium-111-oxine to measure recovery and survival by multiple hit analysis. The in vitro quality was evaluated by total ATP content, hypotonic shock response and extent of shape change with ADP. Ten paired studies were performed with PC from the same donor being stored for 7 d at 22 degrees C in both CPDA-1 plasma and the additive solution. Mean recoveries and survivals were found to be substantially higher with PC stored in the additive solution than with PC stored in CPDA-1 plasma (51.0 +/- 7.8% and 144.1 +/- 15.9 h versus 36.6 +/- 10.7% and 110.4 +/- 31.6 h). The differences were statistically significant (P less than 0.001). The results of the in vitro assays described above parallelled the in vivo results, with statistically significantly superior results (P less than 0.01) for all parameters of PC stored in the additive solution. This study is the first to show that PC quality may be improved and storage extended using an additive solution.

Exploratory studies of extended storage of apheresis platelets in a platelet additive solution (PAS)

“…2,6,26,[39][40][41] de Wildt-Eggen et al provided an excellent review of both in vitro and in vivo results of platelets stored in plasma or PAS. 41 Only 2 prior studies have evaluated platelets stored in PAS beyond 7 days.…”

“…For both stored platelet recoveries and survivals, there are significant correlations with total platelet count of the product, storage volume, morphology score, glucose, Annexin V binding, ESC, HSR, pCO 2 , and pO 2 (all P , .05) (Figure 4A-B). Data are given as the average 6 1 standard error.…”

Photochemical Inactivation of Bacteria and HIV in Buffy–Coat–Derived Platelet Concentrates under Conditions That Preserve in vitro Platelet Function

“…Platelet samples were taken on days 1, 2, 3, 5 and 7 for analysis. The volume, platelet count, platelet dose, pH, partial oxygen pressure (pO 2 ), partial carbon dioxide pressure (pCO 2 ), bicarbonate concentration (HCO 3 – ), glucose, lactate, adenosine triphosphate (ATP), extent of shape change (ESC) [16], and hypotonic shock response (HSR) [17]were measured.…”

Types and sources of fuels for platelets in a medium containing minimal added fuels and a low carryover plasma

“…A wide range of storage formulations have been developed, most of which are effective in replacing a proportion of the plasma, and good storage results have been reported with platelet additive solutions containing acetate Guppy et al, 1990;Whisson et al, 1993) and glucose (Holme et al, 1987b). However, there are still only minimal data on the potential contribution of the plasma to the fuels available in a minimal plasma medium, and on platelet utilization of these plasma-derived fuels.…”

“…There are studies that shed some light on these questions, but most of the media used in these studies contain high contaminations (7-40%) of carryover plasma, and high-glucose-anticoagulant (resulting in glucose concentrations of 4-12 mM) which confound the results (e.g. Holme et al, 1987b;Bertolini et al, 1992). The exceptions are the studies by Shimizu et al (1993) and Whisson et al (1993), in which the carryover plasma is about 1%.…”