Improved hollow-fibre membranes for dye-affinity chromatography

Abstract

Hollow-fibre membranes with different degrees of surface hydrophilicity were obtained by grafting mixtures of glycidyl methacrylate (GMA) and dimethyl acrylamide (DMAA) in various proportions, and Cibacron Blue F3G-A was attached to them through ammonia or glucamine spacers. Membrane hydrophilicity increased with the amount of dimethyl acrylamide in the grafted polymer. As the hydrophilicity increased the permeability decreased from 352 mL/cm2 min MPa for membranes grafted with GMA with ammonia spacer to 12.7 mL/cm2 min MPa for membranes grafted with GMA/DMAA 1/3 with glucamine spacer. Membranes grafted with GMA/DMAA 1/3 with ammonia spacer showed the best performance for BSA and lysozyme adsorption: maximum capacity was 15.3 +/- 2.2 mg BSA/mL membrane and 58.3 +/- 6.6 mg lysozyme/mL membrane while dissociation constants were 0.27 +/- 0.16 and 0.13 +/- 0.12 mg/mL, respectively. Over 80% of adsorbed proteins could be eluted with 2 M NaCl + 20% isopropanol in 20 mM sodium phosphate buffer, pH 7.0.

Bovine lactoferrin purification from whey using Yellow HE-4R as the chromatographic affinity ligand

“…Triazine dyes are pseudobioaffinity ligands widely used for chromatography, which allows the purification of proteins with favorable cost/selectivity ratio . Triazine dyes used in the textile industry in tons are low‐cost ligands.…”

Isolation of lactoferrin from whey by dye-affinity chromatography with Yellow HE-4R attached to chitosan mini-spheres

“…These results show that the maximum adsorption capacity of chitosan matrices was higher than that previously reported for Sepharose matrices (27.82 for Red HE-3B and 40.24 mg g À1 for Yellow HE-4R) (Baieli et al, 2014). C250-Y showed higher maximum adsorption capacity than C250-R. Matrices of the C500 series showed similar values for both dyes, thus evidencing that increased ligand density not always corresponds to a higher adsorption capacity of a protein (Wolman, Smolko, Cascone, & Grasselli, 2005).…”

Chromatographic matrix based on hydrogel-coated reticulated polyurethane foams, prepared by gamma irradiation

“…It has been used to immobilize different kinds of ligands onto microfiltration membranes and applied to purify proteins by specific recognition. The following ligands have been used: iminodiacetic acid (Grasselli et al, 1999), sulfonic groups (Camperi et al, 1999), reactive dyes (Wolman et al, 2005), amino groups (Trelles et al, 2010) and arsenic ligands (Carbajal et al, 2009). In all these cases, the base materials were hydrophobic polymers such as polyethylene or polysulfone, the irradiation solvent was an ethanol/water mixture, and the main grafted monomer was GMA.…”

“…In a previous study, the use of the second monomer DMAA onto SRIGP of polysulfone membranes allowed obtaining an adsorptive membrane containing a surface grafted polymer with improved adsorptive properties. The presence of the hydrophilic monomer DMAA enhanced the adsorptive capacity as a consequence of improved hydrophilic properties of the grafted polymethacrylate layer (Wolman et al, 2005). Therefore, in the present work, we also studied the grafting procedure onto the PVA-coated rPUF using GMA/DMAA.…”