Improved high-temperature ethanol production from sweet sorghum juice using Zymomonas mobilis overexpressing groESL genes

Kaewchana, Anchittha; Techaparin, Atiya; Boonchot, Nongluck; Thanonkeo, Pornthap; Klanrit, Poramate

doi:10.1007/s00253-021-11686-0

Cited by 9 publications

(9 citation statements)

References 66 publications

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“…One of the critical successes in ethanol production under high-temperature conditions is the utilization of high-potential thermotolerant microorganisms [5,6,8]. Several thermotolerant ethanologenic yeasts for HTEF have been reported, such as Kluyveromyces marxianus [8][9][10], Saccharomyces cerevisiae [3,5,6], Pichia kudriavzevii [11], Meyerozyma guilliermondii [12], and Saccharomycodes ludwigii [7]; however, very few reports have considered thermotolerant ethanologenic bacteria, specifically Zymomonas mobilis [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…PCC7120 [27], solvent stress in Clostridium acetobutylicum [28,29], and toxic metabolite stress in Escherichia coli [17]. Recently, we successfully developed a recombinant Z. mobilis overexpressing groESL, designated Z. mobilis R301, which can withstand several stress conditions, including high temperatures, high sugar, and ethanol concentrations of up to 40 • C, 300 g/L, and 102.57 g/L, respectively [16]. This recombinant strain can also utilize sweet sorghum juice (SSJ) as a carbon source for ethanol production.…”

Section: Introductionmentioning

confidence: 99%

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Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant Zymomonas mobilis Overexpressing groESL Genes

Charoenpunthuwong,

Klanrit,

Chamnipa

et al. 2023

Energies

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High-temperature ethanol fermentation (HTEF) using high-potential thermotolerant ethanologenic microorganisms is a promising platform for ethanol production in tropical or subtropical areas. This study aims to evaluate the ethanol production potential of recombinant Zymomonas mobilis R301 overexpressing groESL genes under normal and high-temperature conditions and the expression of genes involved in the heat shock response and ethanol production pathway during ethanol fermentation using sweet sorghum juice (SSJ) as feedstock. Growth characterization analysis revealed that the recombinant Z. mobilis R301 exhibited multi-stress tolerance toward heat, acetic acid, and furfural. Based on the statistical experimental design, the optimum conditions for ethanol production from SSJ by the recombinant R301 at 30 °C were a sugar concentration of 171.67 g/L, cell concentration of 9.42% (v/v), and yeast extract concentration of 10.89 g/L, while those at 40 °C were a sugar concentration of 199.48 g/L, yeast extract concentration of 10.88 g/L, MgSO4 concentration of 1.05 g/L, and initial pH of 6.8. The maximum ethanol concentrations and productivities achieved in this study were 63.26 g/L and 1.17 g/L.h at 30 °C and 58.62 g/L and 1.22 g/L.h at 40 °C. The overexpression of the groES and groEL genes and upregulation of other heat shock-responsive genes at 40 °C enhanced cell growth, viability, and fermentation capacity of recombinant Z. mobilis R301 under heat stress. The current study demonstrated that recombinant Z. mobilis R301 exhibited high potential for ethanol production from SSJ or other sugar-based raw materials under high-temperature conditions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant Zymomonas mobilis Overexpressing groESL Genes

Charoenpunthuwong,

Klanrit,

Chamnipa

et al. 2023

Energies

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“…An optimum yeast mass (40 g) of S. cerevisiae with a distillate volume of 13.6 mL has been found after 96 h of fermentation time, with a distillate/ethanol yield of 15.2 mL. However, under the optimal conditions for fermentation, the volume of the bioethanol distillate was found to be 30 mL with a distillation temperature of 70-80 • C. From this approach to ethanol production, its refractive index (1.354), density (0.367 g/mL), and boiling points (71-72 • C) were reported [113]. In another report, bioethanol production was discussed from the utilization of Sri Lankan rotten fruits (without skin), including jackfruit waste.…”

Section: Bioethanolmentioning

confidence: 92%

“…In another report, bioethanol production was discussed from the utilization of Sri Lankan rotten fruits (without skin), including jackfruit waste. This ethanol was produced in a batch process with an optimization of the fermentation process parameters [112,113]. In the optimization of the fermentation process, some optimization techniques such as the Genetic Algorithm (GA), Response Surface Methodology (RSM), and also Particle Swarm Optimization (PSO) were discussed.…”

Section: Bioethanolmentioning

confidence: 99%

The Utilization of Jackfruit (Artocarpus heterophyllus L.) Waste towards Sustainable Energy and Biochemicals: The Attainment of Zero-Waste Technologies

Sarangi,

Srivastava,

Singh

et al. 2023

Sustainability

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The valorisation of food and fruit wastes has the potential for the production of sustainable energy and biochemicals. Approximately 70% of the weight of the original jackfruit (Artocarpus heterophyllus L.) fruit is lost during its processing as waste in the form of peeled skin and core, both of which have not been utilized and, thus these contribute to disposal as well as pollution issues. The major components such as cellulose and hemicellulose can be easily biologically transformed into bioenergy sources such as ethanol, methanol, and butanol; valuable phenolics and biotechnological products such as pectin, citric acid, bromelain, ferulic acid, and vanillin; and many other products. These residues can also be utilized as essential sources for the biological transformation process, leading to the production of numerous products with added value, such as phenolic antioxidants, phenolic flavour compounds, and organic acids. Thus, the value addition of jackfruit waste can support sustainable solutions towards food and nutritional security. In this way, zero waste can be achieved through novel biorefineries, which are critically highlighted in this paper. Furthermore, novel technologies for the conversion of jackfruit waste are summarized with recent findings.

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“…Overexpression of genes can result in recombinant strains that tolerate a variety of stress levels. For instance, a recombinant Z. mobilis named Z mobilis R301, which overexpresses the groESL genes, can withstand multiple stress conditions, including high temperatures (40 °C), high sugar concentrations (30 g/L), and high ethanol concentrations (102.57 g/L) [ 109 , 110 ]. Additionally, the overexpression of ZMO1721 , a dioxygenase-encoding gene in Z. mobilis ZM4, along with NADH-dependent reductase enzymes involved in the reduction of phenolic aldehydes to phenolic alcohols, enhances the synthesis of phenolic aldehydes (4-hydroxybenzaldehyde, syringaldehyde, and vanillin), glucose consumption, and ethanol production.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive network of stress-induced responses in Zymomonas mobilis during bioethanol production: from physiological and molecular responses to the effects of system metabolic engineering

Asefi,

Nouri,

Pourmohammadi

et al. 2024

Microb Cell Fact

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Nowadays, biofuels, especially bioethanol, are becoming increasingly popular as an alternative to fossil fuels. Zymomonas mobilis is a desirable species for bioethanol production due to its unique characteristics, such as low biomass production and high-rate glucose metabolism. However, several factors can interfere with the fermentation process and hinder microbial activity, including lignocellulosic hydrolysate inhibitors, high temperatures, an osmotic environment, and high ethanol concentration. Overcoming these limitations is critical for effective bioethanol production. In this review, the stress response mechanisms of Z. mobilis are discussed in comparison to other ethanol-producing microbes. The mechanism of stress response is divided into physiological (changes in growth, metabolism, intracellular components, and cell membrane structures) and molecular (up and down-regulation of specific genes and elements of the regulatory system and their role in expression of specific proteins and control of metabolic fluxes) changes. Systemic metabolic engineering approaches, such as gene manipulation, overexpression, and silencing, are successful methods for building new metabolic pathways. Therefore, this review discusses systems metabolic engineering in conjunction with systems biology and synthetic biology as an important method for developing new strains with an effective response mechanism to fermentation stresses during bioethanol production. Overall, understanding the stress response mechanisms of Z. mobilis can lead to more efficient and effective bioethanol production. Graphical Abstract

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Improved high-temperature ethanol production from sweet sorghum juice using Zymomonas mobilis overexpressing groESL genes

Cited by 9 publications

References 66 publications

Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant Zymomonas mobilis Overexpressing groESL Genes

Optimization Condition for Ethanol Production from Sweet Sorghum Juice by Recombinant Zymomonas mobilis Overexpressing groESL Genes

The Utilization of Jackfruit (Artocarpus heterophyllus L.) Waste towards Sustainable Energy and Biochemicals: The Attainment of Zero-Waste Technologies

Comprehensive network of stress-induced responses in Zymomonas mobilis during bioethanol production: from physiological and molecular responses to the effects of system metabolic engineering

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