Improved high-performance liquid chromatographic method for the determination of coenzyme Q10 in plasma

Grossi, G.; Bargossi, A.; Fiorella, P.L.; Piazzi, S.; Battino, Maurizio; Bianchi, Giampaolo

doi:10.1016/0021-9673(92)80289-7

Cited by 82 publications

(54 citation statements)

References 12 publications

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“…However, these methods were timeconsuming, complicated and lacking validation for measuring milk CoQ 10 . The first study was reported by Compagnoni et al (2004), who adopted a method of Grossi et al (1992) to measure CoQ 10 levels in 15 samples. In brief, samples were treated with methanol and extracted with n-hexane.…”

Section: Discussionmentioning

confidence: 99%

Determination of coenzyme Q10 in human breast milk by high-performance liquid chromatography

Tang

Miles

Steele

et al. 2006

Biomed. Chromatogr.

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An isocratic HPLC method was developed for the determination of coenzyme Q(10) (CoQ(10)) in human breast milk. After a single-step liquid-liquid extraction, the milk extract was injected directly into the HPLC system. The analytical method is based on pre-column inline treatment of CoQ(10). Chromatographic separation of CoQ(10) and coenzyme Q(9) (CoQ(9)) internal standard was achieved using a reversed-phase Microsorb-MV C(18) analytical column. CoQ(10) and CoQ(9) were monitored by an electrochemical detector (ECD). An excellent linearity (r = 0.999) was observed for CoQ(10) in the concentration range 0.06-2.5 micromol L(-1) in breast milk. The limit of quantitation (LOQ) was 60 nmol L(-1). Coefficients of variations (CVs) for intra-day and inter-day assay precisions were less than 5%. A total of 194 breast milk samples were analyzed for the CoQ(10) concentration; the mean value was 0.32 +/- 0.21 micromol L(-1).

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Section: Discussionmentioning

confidence: 99%

Determination of coenzyme Q10 in human breast milk by high-performance liquid chromatography

Tang

Miles

Steele

et al. 2006

Biomed. Chromatogr.

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“…There are some studies about plasma or serum CoQ 10 concentrations, which are usually employed for the assessment of CoQ 10 status in humans, primarily because of the ease of the sample collection. There are several excellent methods based on HPLC for the analysis of CoQ 10 in plasma or serum (Lang & Parcker 1987;Grossi et al 1992;Gvozdjáková et al 2000;Tang et al 2001).…”

Section: Levels Of Coq 10 In the Human Organismmentioning

confidence: 99%

Nourishing and health benefits of coenzyme Q<sub>10</sub>

Borekova¹,

Hojerová²,

Koprda³

et al. 2008

Czech J. Food Sci.

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“…The mobile phase was methanol:ethanol (40:60). The column was maintained at a flow rate of 1.5 ml/min and the total oxidized CoQ10 was detected at 275 nm [17]. Serum spiked with purified CoQ10 was used as quality control.…”

Section: Methods Of Analysismentioning

confidence: 99%

Relative Bioavailability and Antioxidant Potential of Two Coenzyme Q10 Preparations

et al. 2003

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Coenzyme Q10 (CoQ10) is synthesized by the human body and found in certain foods. Daily supplementation of CoQ10 could protect against heart disease but the bioavailability of CoQ10 supplements depends on the formulation taken. We compared the bioavailability and antioxidant properties of two commercial CoQ10 formulations, a commercial grade CoQ10 powder (commercial grade CoQ) and a new BT-CoQ10 BIO-TRANSFORMED^® (BT-CoQ10) obtained by fermentation of a soy-based, CoQ10-rich media with baker’s yeast. Eleven healthy individuals participated in a randomized two-way crossover trial, with a 3-week washout period. Capsules containing 300 mg of either BT-CoQ10 or commercial grade CoQ10 were given daily for 1 week and multiple blood samples were taken for CoQ10, glutathione and glutathione peroxidase (GPx) determination. In 3 subjects, baseline plasma CoQ10 levels were lower prior to BT than prior to commercial grade CoQ treatment. In the remaining participants, ingestion of BT vs. commercial grade CoQ significantly increased maximum plasma CoQ10 concentration (+126%, p = 0.04) and tended to increase CoQ10 area under the curve from 0 to 24 h (+160%, p = 0.07). One week of treatment with each formulation increased plasma CoQ10 but did not alter plasma glutathione or GPx activity. The enhanced bioavailability of the BT product might be due to its predominantly reduced, hydrophilic membrane-complex form.

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Improved high-performance liquid chromatographic method for the determination of coenzyme Q10 in plasma

Cited by 82 publications

References 12 publications

Determination of coenzyme Q10 in human breast milk by high-performance liquid chromatography

Determination of coenzyme Q10 in human breast milk by high-performance liquid chromatography

Nourishing and health benefits of coenzyme Q<sub>10</sub>

Relative Bioavailability and Antioxidant Potential of Two Coenzyme Q10 Preparations

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