Improved Growth of Tissue Cultures of the Onion, <i>Allium cepa</i>

Dunstan, David I.; Short, K. C.

doi:10.1111/j.1399-3054.1977.tb01525.x

Cited by 163 publications

(63 citation statements)

References 11 publications

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“…Novak (1990) noted that B5 (Gamborg et al 1968)-based media have been in common use for Allium tissue cultures. Dunstan & Short (1977) obtained a substantial increase in A. cepa callus growth rate after increasing the ammonium, phosphate and nitrate levels of the B5 medium. Their so-called BDS medium has served as the basal medium in a number of subsequent studies (Phillips & Luteyn 1983;Phillips & Hubstenberger 1987;Lu et al 1989), Somatic embryogenesis has been observed in callus cultures of A. cepa (Dunstan & Short 1977;Phillips & Luteyn 1983); A. fistulosum (Phillips & Hubstenberger 1987;Shahin & Kaneko 1986) and in F1 hybrids between A. cepa and A. fistulosum (Shahin & Kaneko 1986;Phillips & Hubstenberger 1987;Lu et al 1989).…”

Section: Introductionmentioning

confidence: 98%

High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species

Valk¹,

Scholten²,

Verstappen³

et al. 1992

Plant Cell Tiss Organ Cult

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High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species Valk, P. van der; Scholten, O.E.; Verstappen, F.; Jansen, Ritsert; Dons, J.J.M. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. The plant regeneration ability of zygotic embryo-derived callus cultures was studied for 12 A. cepa varieties and accessions, two A. fistulosum varieties, one A. fistulosum x A. cepa interspecific hybrid and two A. porrum varieties. Compact embryogenic callus was induced on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid. The embryogenic calluses of all three Allium species were similar in appearance. For all accessions tested plants could be regenerated at a high frequency from this compact callus through somatic embryogenesis, when using kinetin supplemented MS medium (regeneration medium). Addition of abscisic acid to the regeneration medium stimulated the formation of both somatic embryos and shoots for a number of varieties. Concerning shoot regeneration from callus cultures, significant differences existed between genotypes of all accessions except one.Abbreviations: 2,4-D-2,4-dichlorophenoxyacetic acid, VDH-Van Der Have Seed company

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Section: Introductionmentioning

confidence: 98%

High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species

Valk¹,

Scholten²,

Verstappen³

et al. 1992

Plant Cell Tiss Organ Cult

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“…Podem-se encontrar vários meios para tal finalidade, como MS (Murashige & Skoog, 1962), WPM [(Wood Plant Medium) Lloyd & McCown (1980)], MSM (meio MS modificado por Monteiro-Hara, 2000), BDS (Dunstan & Short, 1977) e o meio DSD1 (Silva & Doazan, 1995).…”

Section: Introductionunclassified

Micropropagação do maracujazeiro-do-sono

Santos¹,

Ramos

Pasqual³

et al. 2010

Rev. Ceres

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Micropropagation of Passiflora setacea DC.Propagation of Passiflora setacea DC. is extremely difficult, therefore tissue culture techniques become a viable alternative. The objective of this study was to evaluate the in vitro germination and determine the best culture medium for micropropagation. This work was carried out in two stages; in the first, seeds were placed in ½ MS culture medium and 30 g L -1 of sucrose, distributed in tubes and supplemented with different AG 3 concentrations. The pH of culture medium was adjusted for 5.8 before adding 6.0 mg L -1 of agar. After seed inoculation, the cultures were kept in a growth room under 35 µmol.m -2 .s -1 , 26±1ºC and photoperiod of 16 hours. Just after germination, the seedlings were transferred to tubes containing ½ MS, constituting a second experiment, in order to test culture media with different sucrose concentrations. The experiments were arranged in a complete randomized design, in a 3x3 factorial, with four repetitions and 15 seeds/plot (first experiment) and a 4x4 factorial, with four repetitions and 3 plants/plot (second experiment). The best results for micropropagation were obtanied in MSM medium with 28.51 and 28.74 g L -1 of sucrose. The highest germination speed index was obtained with 20 mg L -1 of AG 3 combined with seed tip scarification.

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“…On the other hand, Luciani et al (2006) tested different explants for micropropagation of variety 069, which were cultivated on BDS medium (Dustan & Short, 1977), supplemented with picloram, 2,4-D and BA. The basal plates and meristems resulted in the highest values of shoot regeneration, and 2,4-D proved to be better than picloram for inducing callus and shoot formation.…”

Section: Organogenesismentioning

confidence: 99%