Improved Growth and High Inheritance of Melanocortin-4 Receptor (mc4r) Mutation in CRISPR/Cas-9 Gene-Edited Channel Catfish, Ictalurus punctatus

Coogan, Michael; Alston, Veronica; Su, Baofeng; Khalil, Karim; Elaswad, Ahmed; Khan, Mohd Golam Quader; Johnson, Andrew; Xing, De; Li, Shangjia; Wang, Jinhai; Simora, Rhoda Mae C.; Lu, Cuiyu; Page-McCaw, Patrick; Chen, Wenbiao; Michel, Maximilian; Wang, Wenwen; Hettiarachchi, Darshika; Hasin, Tasnuba; Butts, Ian A. E.; Cone, Roger D.; Dunham, Rex A.

doi:10.1007/s10126-022-10146-8

Cited by 10 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RCC mc3r +/− also exhibited slightly more visceral fat mass compared to WT (Figures 7E-H), indicating that MC3R and MC4R played an essential role in lipid accumulation and the role of MC4R was more significant than that of MC3R, consistent with previous studies in mice (3,46). The mc4r mutants have been researched in several species including fish, pigs, and chickens (3,(47)(48)(49)(50)(51)(52). In channel catfish, the body weights of mc4r-deficient fish exhibited 20%-38% improvement compared to WT (49).…”

Section: Discussionsupporting

confidence: 88%

Cloning, distribution, and effects of growth regulation of MC3R and MC4R in red crucian carp (Carassius auratus red var.)

Huang,

Deng,

Yang

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

BackgroundMelanocortin-3 and -4 receptors (MC3R and MC4R), G protein-coupled receptors, play vital roles in the regulation of energy homeostasis. To understand the functions of mc3r and mc4r in the energy homeostasis of red crucian carp (Carassius auratus red var., RCC), we cloned mc3r and mc4r, analyzed the tissue expression and localization of the genes, and investigated the effects of knockout of mc3r (mc3r+/-) and mc4r (mc4r+/-) in RCC. ResultsThe full-length cDNAs of RCC mc3r and mc4r were 1459 base pairs (bp) and 1894 bp, respectively. qRT-PCR indicated that mc3r and mc4r were profusely expressed in the brain, but lower expressed in the periphery tissues. ISH revealed that mc3r and mc4r were located in NPP, NPO, NAPv, NSC, NAT, NRL, NLTl, and NLTp of the brain, suggesting that mc3r and mc4r might regulate many physiological and behavioral aspects in RCC. To further verify the roles of mc3r and mc4r in energy homeostasis, the mc3r+/- and mc4r+/- fish were obtained by the CRISPR/Cas9 system. The average body weights, total lengths, body depths, and food intake of mc4r+/- fish were significantly higher than those of mc3r+/- and the normal wild-type (WT) fish, but there was no difference between the mc3r+/- and WT fish, indicating that the RCC phenotype and food intake were mainly influenced by mc4r but not mc3r. Interestingly, mc4r+/- fish displayed more visceral fat mass than mc3r+/- and WT fish, and mc3r+/- fish also exhibited slightly more visceral fat mass compared to WT. RNA-seq of the liver and muscle revealed that a large number of differentially expressed genes (DEGs) differed in WT vs. mc3r+/-, WT vs. mc4r+/-, and mc3r+/- vs. mc4r+/-, mainly related to lipid, glucose, and energy metabolism. The KEGG enrichment analysis revealed that DEGs were mainly enriched in pathways such as steroid biosynthesis, fatty acid metabolism, fatty acid biosynthesis, glycolysis/gluconeogenesis, wnt signaling pathway, PPAR signaling pathway, and MAPK signaling pathway, thereby affecting lipid accumulation and growth. ConclusionIn conclusion, these results will assist in the further investigation of the molecular mechanisms in which MC3R and MC4R were involved in the regulation of energy homeostasis in fish.

show abstract

Section: Discussionsupporting

confidence: 88%

Cloning, distribution, and effects of growth regulation of MC3R and MC4R in red crucian carp (Carassius auratus red var.)

Huang,

Deng,

Yang

et al. 2024

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

“…Furthermore, to enhance the quality of the pork, genes encoding enzymes involved in meat tenderization, such as calpastatin and μ-calpain, have been successfully modified [ 57 ]. Moreover, reducing fat deposition and increasing feed efficiency have been achieved by targeting the Stearoyl-CoA desaturase (SCD) gene, which is involved in the synthesis of fatty acids in pigs [ 66 ]. The MC4R gene involved in appetite regulation in chickens has been targeted to improve feed efficiency and reduce feed intake [ 35 ] ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

Advancement of animal and poultry nutrition: Harnessing the power of CRISPR-Cas genome editing technology

Mishu,

Nath,

Sohidullah

et al. 2024

J Adv Vet Anim Res

View full text Add to dashboard Cite

CRISPR-associated proteins and clustered regularly interspaced short palindromic repeats (CRISPR-Cas) technology has emerged as a groundbreaking advancement in animal and poultry nutrition to improve feed conversion efficiency, enhance disease resistance, and improve the nutritional quality of animal products. Despite significant advancements, there is a research gap in the systematic understanding and comprehensive use of the CRISPR-Cas method in animal and poultry nutrition. The purpose of this study is to elucidate the latest advancements in animal and poultry nutrition through CRISPR-Cas genome editing technology, focusing on gene manipulation in metabolism, immunity, and growth. Following preferred reporting items in meta-analysis and systematic reviews guidelines, we conducted a systematic search using several databases, including Scopus, PubMed, and Web of Science, until May 2024, and finally, we included a total of 108 articles in this study. This article explores the use of the CRISPR-Cas system in the advancement of feed additives like probiotics and enzymes, which could reduce the use of antibiotics in animal production. Furthermore, the article discusses ethical and regulatory issues related to gene editing in animal and poultry nutrition, including concerns about animal welfare, food safety, and environmental impacts. Overall, the CRISPR-Cas system holds substantial promise to overcome the challenges in modern animal agriculture. By enriching the nutritional quality of animal products, increasing disease resistance, and improving feed efficiency, it offers sustainable and cost-effective solutions that can revolutionize animal and poultry nutrition

show abstract

“…Biotechnology, including gene banking and transgenic fish, has the potential to transform aquaculture practices. These innovative techniques improve catfish growth and disease resistance and ultimately increase aquaculture yield [ 30 , 31 ]. Additionally, catfish diets vary based on their age, size, and species.…”

Section: Introductionmentioning

confidence: 99%

Catfishes: A global review of the literature

Chandra Segaran,

Azra,

Piah

et al. 2023

Heliyon

View full text Add to dashboard Cite

Improved Growth and High Inheritance of Melanocortin-4 Receptor (mc4r) Mutation in CRISPR/Cas-9 Gene-Edited Channel Catfish, Ictalurus punctatus

Cited by 10 publications

References 41 publications

Cloning, distribution, and effects of growth regulation of MC3R and MC4R in red crucian carp (Carassius auratus red var.)

Cloning, distribution, and effects of growth regulation of MC3R and MC4R in red crucian carp (Carassius auratus red var.)

Advancement of animal and poultry nutrition: Harnessing the power of CRISPR-Cas genome editing technology

Catfishes: A global review of the literature

Contact Info

Product

Resources

About