Improved gas chromatography packings with fluidized drying

d/(g cm"3) = 0.91694 -(8.149 X 10'4Xf/°C) -(5.1 X 10"7Xf/°C)2 = 3 X 10"5 g cm"3(1) where d is the density at temperature t. Values calculated from eq 1 are listed in column 3 of Table I. The good agreement with the experimental values demonstrates the concordance of both data sets. Column 5 contains literature data. The single value at 27 °C reported by Scatchard and Wilson is in excellent agreement with our value interpolated by using eq 1; these authors used essentially the same purification procedure as we used. The four values of Onken ( 7) are in poor agreement with our values. This discrepancy in d and the previously mentioned discrepancy in n2^a re consistent with the presence of a small amount of water contamination in his samples. The effect of this would be to lower the refractive index and raise the density in the way observed. Glossary d density, g cm"3 t temperature, °C standard deviation Literature Cited (1) Guzman, F.; McLure, I. A. Int. Data Ser. B 1980, 109.(2) Weast, R. C" Ed. "Handbook of Chemistry and Physics", 55th ed.:

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“…The slurry was then transferred to a fluidizer similar to that used by Kruppa et al (39). Excess liquid was drawn off by the house vacuum.…”

Section: Methodsmentioning

confidence: 99%

Limiting activity coefficients of nonpolar and polar solutes in both volatile and nonvolatile solvents by gas chromatography

Thomas¹,

Newman²,

Long³

et al. 1982

J. Chem. Eng. Data

153

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“…Glass columns, 1.85-m X 4.0-mm i.d., were packed with 70/80 mesh Chromosorb W (AW) (DMCS) coated with 10% (w/w) stabilized diethyleneglycolsuccinate (Analabs, Inc.). During the last 2 years of the study column packings were prepared by the filtration-fluidization technique (Kruppa et al, 1967).…”

Section: Methodsmentioning

confidence: 99%

Varietal differences and seasonal effects on fatty acid composition and stability of oil from 82 peanut genotypes

Worthington¹,

Hammons²,

Allison³

1972

J. Agric. Food Chem.

100

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“…The column material and conditions employed are similar to those described by Albersheim et al (4). The column material is a liquid phase consisting of 0.2% (poly)ethylene glycol adipate, 0.2% (poly)ethylene glycol succinate, and 0.4% XF-1 150 silicone oil coated on Gas-Chrom P (100-200 mesh) by means of a "Hi-Eff" fluidizer (32 For the analysis of the alditol acetates formed from the neutral sugars, the following temperature program is employed: 7 min postinjection hold at 120 C, followed by a linear 1 C per min temperature rise to 180 C. The temperature is then maintained at 180 C for approximately 6 min or until the elution of the final sample component, myoinositol hexaacetate, is completed. For the analysis of the alditol acetates formed from the uronic acids, a linear temperature rise of 1 C per min from 150 to 180 C is employed with an approximately 6 min hold at the upper temperature limit.…”

Section: Plantmentioning

confidence: 99%

A Gas Chromatographic Method for the Determination of Aldose and Uronic Acid Constituents of Plant Cell Wall Polysaccharides

1972

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A major problem in determining the composition of plant cell wall polysaccharides has been the lack of a suitable method for accurately determining the amounts of galacturonic and glucuronic acids in such polymers. A Two methods for the enzymatic determination of uronic acids have also been described. The first of these methods, employing an NADH-linked enzyme system from Bacillus polymyxa for the reduction of D-galacturonic acid, has been used for the quantitative determination of monomeric galacturonic acid in the presence of large amounts of galacturonic acid oligomers (40). An NAD-linked uronate dehydrogenase from Pseudomonas syringae has also been used for the determination of D-galacturonic acid (8, 9). In this case both D-galacturonic and D-glucuronic acids are oxidized, and what is determined is the sum of the concentrations of the two acids. Hence, it is impossible. by this method, to distinguish these two compounds, both of which are common constituents of plant polysaccharides.With the development of gas-liquid chromatography of carbohydrates (11, 23. 24, 61, 67), this powerful method became available for the quantitative analysis of monosaccharide mixtures (54). These techniques have been refined by a number of investigators to the point that the gas chromatographic analysis of the neutral sugar components of plant cell wall polysaccharides is accurate, efficient, and convenient (4, 13-16, 19, 26, 27, 29. 39, 47, 57, 59). Of the various techniques available, those involving the determination of neutral sugars following conversion to the corresponding alditol acetates (4, 13, 16, 59) have proven the most satisfactory for determining cell wall polysaccharide composition.The gas chromatographic determination of the uronic acids, the other major component of plant cell wall polysaccharides, has received less attention. Tamura and Imanari (28,62) have investigated a variety of glucuronide derivatives, and have concluded that the trimethylsilyl ethers are satisfactory for gas chromatographic analysis of such compounds. Other investigators have examined the gas chromatographic behavior of the trimethylsilyl ether derivatives of iduronic, glucuronic (34,35), and galacturonic (56) acids. Separation of the anomeric forms of uronic acids as trimethylsilyl derivative has also been explored (17. 30. 31, 50, 63, 70, 71). In addition, Raymond and Nagel (52) have demonstrated the feasibility of separating the trimethylsilyl derivatives of galacturonic acid oligomers by means of gas chromatography. A major disadvantage in the use of trimethylsilyl derivatives for cell wall polysaccharide analysis lies in the difficulty of achieving satisfactory separations of the derivatives of each of the aldoses and uronic acids found in plant cell walls. No gas chromatographic column material which permits such a separation is available at present.Another approach to hexuronic acid analysis has been presented by Perry and Hulyalkar (48) in which salts of the uronic acids are converted to aldono-1,4-lactones from which tr...

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Improved gas chromatography packings with fluidized drying

Cited by 32 publications

References 3 publications

Limiting activity coefficients of nonpolar and polar solutes in both volatile and nonvolatile solvents by gas chromatography

Limiting activity coefficients of nonpolar and polar solutes in both volatile and nonvolatile solvents by gas chromatography

Varietal differences and seasonal effects on fatty acid composition and stability of oil from 82 peanut genotypes

A Gas Chromatographic Method for the Determination of Aldose and Uronic Acid Constituents of Plant Cell Wall Polysaccharides

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