Improved functionalization of electrospun PLLA/gelatin scaffold by alternate soaking method for bone tissue engineering

Jaiswal, Akhilesh; Kashte, Shivaji; Soni, Vivek; Bellare, Jayesh

doi:10.1016/j.apsusc.2012.12.152

Cited by 75 publications

(49 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, an increase in fiber diameter after crosslinking is observed. Other authors observed similar findings, particularly evident in high content gelatin containing scaffolds (Jaiswal et al, ; Piccirillo et al, ). Possibly, the high ethanol concentration (85%, vol/vol) used during crosslinking determined a shrinking effect on gelatin, due to de‐swelling.…”

Section: Discussionsupporting

confidence: 63%

“…After immersion in SBF for 28 days, the S1 scaffold showed a flattening and partial degradation of the fibers, while the S2 and S3 fibers are completely coated by a mineral layer (Figure 3b). This observation suggests that the mineral layer formed on S2 and S3 scaffolds prevents degradation in aqueous solution (Hong, Reis, & Mano, ; Jaiswal, Kadam, Soni, & Bellare, ).…”

Section: Resultsmentioning

confidence: 98%

See 1 more Smart Citation

Electrospun poly(d,l‐lactide)/gelatin/glass‐ceramics tricomponent nanofibrous scaffold for bone tissue engineering

Bochicchio

Barbaro

Bonis

et al. 2020

J Biomedical Materials Res

View full text Add to dashboard Cite

Electrospun scaffolds are emerging as extracellular matrix (ECM)mimicking structures for tissue engineering thanks to their nanofibrous architecture. For the development of suitable electrospun scaffolds for bone tissue engineering, the addition of inorganic components has been implemented with the aim to confer important bioactivity like osteoinduction, osteointegration, and cell adhesion to the scaffolds. In this context, we propose a tricomponent electrospun scaffold composed of poly(d,l‐lactide), gelatin and RKKP glass‐ceramics. The bioactive RKKP glass‐ceramic system has attracted interest, due to the presence of ions such as La3+ and Ta5+, which turned out to be valuable as growth supporting agents for bones. In this work, RKKP glass‐ceramics were embedded inside the microfibers of electrospun scaffolds and the structural and biological properties were investigated. Our results showed that the glass‐ceramic microparticles were uniformly distributed in the fibrous structure of the scaffold. Furthermore, the glass‐ceramics promoted biomineralization of the scaffolds and improved cell viability and osteogenic differentiation. The mineralized layer formed on RKKP‐containing scaffolds after incubation in simulated body fluid medium has been shown to be hydroxyapatite by Raman spectroscopy and X‐ray diffraction. The results on differentiation studies of canine adipose‐derived mesenchymal stem cells grown on the electrospun scaffolds suggest that on varying the content of RKKP in the scaffold, it is possible to drive the differentiation toward chondrogenic or osteogenic commitment. The presence of ions, like La3+ and Ta5+, in the RKKP embedded polymeric composite scaffolds could play a role in supporting cell growth and promoting differentiation.

show abstract

Section: Discussionsupporting

confidence: 63%

Section: Resultsmentioning

confidence: 98%

Electrospun poly(d,l‐lactide)/gelatin/glass‐ceramics tricomponent nanofibrous scaffold for bone tissue engineering

Bochicchio

Barbaro

Bonis

et al. 2020

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…The roughness of a biomaterial-based graft is an important factor due to its e ect on various cellular behaviors such as protein adsorption, cell attachment, and cell proliferation [12,13]. Similar to the results reported by Milleret et al [14], surface roughness increased with increasing constituent ber diameter (Figure 2).…”

Section: Discussionsupporting

confidence: 79%

Impact of electrospun Tecophilic/gelatin scaffold biofunctionalization on proliferation of vascular smooth muscle cells

2017

View full text Add to dashboard Cite

Abstract. Nano brous composite sca olds based on Tecophilic (TP) and gelatin (gel) were electrospun, and further modi cation of their surfaces was performed by the adsorption of gelatin or bronectin biomolecules. The ability of coated sca olds to alter the proliferation rate of Smooth Muscle Cells (SMCs) was investigated via various assays and compared to cell proliferation on non-coated sca olds. The results con rmed the potential of both coated and non-coated composite sca olds to support SMC growth. Although the presence of bronectin increased the proliferation, adsorbed gelatin could reduce the proliferation of SMCs. The success of a tissue-engineered vascular graft depends on the ability of the sca old to control the proliferation rate of SMCs; thus, our study provides a better insight into the fabrication of functional constructs for vascular regeneration.

show abstract

“…Jaiswal et al concentrated their studies on comparison of cell viability on PLLA with PLLA/HA/gelatin. They observed better cell proliferation on PLLA compared with the hybrid scaffold, but better differentiation on the hybrid scaffold than the other . Other similar studies performed by Samiei et al and Feng et al also showed less proliferation on the scaffold containing HA.…”

Section: Discussionmentioning

confidence: 77%

Surface mineralized hybrid nanofibrous scaffolds based on poly(l‐lactide) and alginate enhances osteogenic differentiation of stem cells

Ataie

Shabani

Seyedjafari

2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Surface mineralized nanofibrous scaffolds hold great potential for bone tissue engineering applications. In this study, a new hybrid nanofibrous scaffold composed of alginate/poly(l‐lactide) nanofibers was fabricated using electrospinning method and then crosslinking process was employed. Hydroxyapatite crystal formation took place using in situ precipitation by immersion of the scaffolds in simulated body fluid solution for 10 days at 37°C. The morphologies of the scaffolds were observed using scanning electron microscope. Hydroxyapatite crystal formation on the surface of nanofibers was confirmed using scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, Fourier transform infrared spectroscopy and X‐ray diffraction analysis. The biocompatibility of the fabricated scaffolds was evaluated using mesenchymal stem cell culture and MTT assay. According to alkaline phosphatase activity and calcium content assays, it was concluded that the osteogenic differentiation of stem cells was considerable on scaffolds containing hydroxyapatite crystals in comparison with other specimens. The results showed biocompatibility of the scaffolds and their support from stem cell adhesion, growth and osteogenic differentiation, so the hydroxyapatite‐coated poly(l‐lactide)/alginate hybrid nanofibrous scaffolds hold suitable characteristics for bone tissue engineering applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 586–596, 2019.

show abstract

Improved functionalization of electrospun PLLA/gelatin scaffold by alternate soaking method for bone tissue engineering

Cited by 75 publications

References 59 publications

Electrospun poly(d,l‐lactide)/gelatin/glass‐ceramics tricomponent nanofibrous scaffold for bone tissue engineering

Electrospun poly(d,l‐lactide)/gelatin/glass‐ceramics tricomponent nanofibrous scaffold for bone tissue engineering

Impact of electrospun Tecophilic/gelatin scaffold biofunctionalization on proliferation of vascular smooth muscle cells

Surface mineralized hybrid nanofibrous scaffolds based on poly(l‐lactide) and alginate enhances osteogenic differentiation of stem cells

Contact Info

Product

Resources

About