Improved fat transplantation survival by using the conditioned medium of vascular endothelial growth factor transfected human adipose‐derived stem cells

Abstract

Autologous fat transplantation has been applied widely in clinic. However, the low survival rate is still a problem to be solved. Studies shows that the human adipose-derived stem cells (ADSCs) transfected by vascular endothelial growth factor (VEGF) can improve the survival rate of autologous fat transplantation. Our study is to evaluate the effects of the conditioned medium of VEGF-transfected human adipose-derived stem cells (VEGF-ADSCs-CM) on fat transplantation. ADSCs were isolated and transfected with MOI = 40. The study was divided into three groups, VEGF-ADSCs-CM group, normal-ADSCs-CM group and control group. The conditioned media for VEGF-ADSCs-CM group and normal-ADSCs-CM group were collected, and then mixed with fat, with the mixtures being injected into the back of nude mice. On 4, 7, 15, 30, 60 days after transplantation, the grafts were evaluated on the wet weight, histology, ELISA and western blot. As the results revealed, the survival rate of VEGF-ADSCs-CM group was highest with the best fat cell morphology, and the VEGF secretion of VEGF-ADSCs-CM group was also highest. Therefore, our study demonstrates that VEGF-ADSCs-CM can improve the survival rate of fat transplantation effectively, and VEGF-ADSCs-CM can be regarded as an effective assisted method for fat transplantation.

Crucial lncRNAs associated with adipocyte differentiation from human adipose-derived stem cells based on co-expression and ceRNA network analyses

“…Also, PTPRB was reported to suppress the tyrosine phosphorylation of VEGFR2 during adipocyte differentiation (Kim et al, 2019). Generally, VEGF functions by binding with VEGFR2, while transfection of VEGF to ASCs increased fat cell survival (Zhang et al, 2017). These findings suggest PTPRB may also be downregulated to promote VEGF secretion and activate its mediated pathways, ultimately inducing adipogenic differentiation from ASCs.…”

Effects of VEGF‐ANG‐1‐PLA nano‐sustained release microspheres on proliferation and differentiation of ADSCs

“…Recently, accumulating evidence has strongly implied that several cytokines are essential to improve the survival rate of vascular endothelial cells (VECs). Vascular endothelial growth factor (VEGF) is one of the most important growth factors to promote angiogenesis, since it was proven to promote VECs proliferation and migration, facilitate blood vessel formation, extend the lifespan of VECs, and enhance vascular permeability (Zhang et al, ). Several studies suggested that VEGF is a highly secreted mitogenic growth factor, which specifically acts on VECs, induces matrix metalloproteinase (MMP) and collagenase high expression, and promotes the degradation of extracellular matrix (ECM), and then facilitates the migration of ECs and causes the generation of new capillaries (Podar et al, ).…”

Application of Adipose Stem Cell Glue in Facial and Breast Plastic

“…Because it is not easy to obtain ADSCs from the donor site during liposuction, the additional ADSCs obtained are diffused into the liposuction liquid, resulting in a very small amount of ADSCs in the adipose tissue obtained, resulting in a decrease in the survival rate of fat particle transplantation [7]. Therefore, adding enough ADSCs to the transplanted fat can significantly reduce the incidence of fat transplantation and increase the survival rate of fat [7,8]. SVF-gel enriches ADSCs by purely physical means, which is safe [9].…”