Improved Extraction Procedure for Carotenoids from Human Milk

Schweigert, Florian J.; Hurtienne, Andrea; Bathe, Katharina

doi:10.1024/0300-9831.70.3.79

Cited by 34 publications

(28 citation statements)

References 9 publications

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“…Plasma a-tocopherol, being stable at 280°C for at least 2.3-15 years, was determined using a modified gradient reversed-phase HPLC system (Waters, Eschborn, Germany) after organic extraction as previously described (15). Results were compared with standard reference material (968a fatsoluble vitamins in human serum; National Institute of Standards and Technology, Gaithersburg, USA).…”

Section: Data Collectionmentioning

confidence: 99%

Low Plasma α-Tocopherol Concentrations and Adverse Clinical Outcomes in Diabetic Hemodialysis Patients

Espe

Raila

Henze

et al. 2013

Clinical Journal of the American Society of Nephrology

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SummaryBackground and objectives Trials with the antioxidant vitamin E have failed to show benefit in the general population. Considering the different causes of death in ESRD, this study investigated the association between plasma concentrations of a-tocopherol and specific clinical outcomes in diabetic hemodialysis patients.Design, settings, participants, & measurements In 1046 diabetic hemodialysis patients (participants of the German Diabetes and Dialysis Study), a-tocopherol was measured in plasma by reversed-phase HPLC. By Cox regression analyses, hazard ratios were determined for prespecified end points according to baseline plasma a-tocopherol levels: sudden death (n=134), myocardial infarction (n=172), stroke (n=89), combined cardiovascular events (n=398), fatal infection (n=107), and all-cause mortality (n=508).Results Patients had a mean age of 6668 years, and mean plasma a-tocopherol level was 22.869.6 mmol/L. Levels of a-tocopherol were highly correlated to triglycerides (r=0.63, P,0.001). Patients in the lowest a-tocopherol quartile had (in unadjusted analyses) a 79% higher risk of stroke and a 31% higher risk of all-cause mortality compared with patients in the highest quartile. The associations were attenuated after adjustment for confounders (hazard ratio stroke =1.56, 95% confidence interval=0.75-3.25; hazard ratio mortality =1.22, 95% confidence interval=0.89-1.69, respectively). There was no association between a-tocopherol and myocardial infarction, sudden death, or infectious death.Conclusions Plasma a-tocopherol concentrations were not independently associated with cardiovascular outcomes, infectious deaths, or all-cause mortality in diabetic hemodialysis patients. The lack of association can partly be explained by a confounding influence of malnutrition, which should be considered in the planning of trials to reduce cardiovascular risk in dialysis patients.

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Section: Data Collectionmentioning

confidence: 99%

Low Plasma α-Tocopherol Concentrations and Adverse Clinical Outcomes in Diabetic Hemodialysis Patients

Espe

Raila

Henze

et al. 2013

Clinical Journal of the American Society of Nephrology

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“…Retinol was determined using a modified gradient reversed-phase HPLC-system (Waters, Eschborn, Germany) after organic extraction as previously described (33). Briefly, retinol was extracted twice with n-hexane from serum and separated on a C30 column (5 m, 250 ϫ 4.6 mm; YMC, Wilmington, NC).…”

Section: Subjectsmentioning

confidence: 99%

High-Normal C-Reactive Protein Levels Do Not Affect the Vitamin A Transport Complex in Serum of Children and Adolescents with Type 1 Diabetes

et al. 2007

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Type 1 diabetes is associated with the presence of inflammation, which in turn affects parameters used to assess the vitamin A status. In the present study, we evaluated the influence of inflammatory status on retinol, retinol-binding protein 4 (RBP4), and transthyretin (TTR) in children and adolescents with type 1 diabetes. A total of 40 children with type 1 diabetes (median age, 14.2 y; median BMI-SDS, 0.53; median diabetes duration, 5.8 y; median HbA 1c , 7.3%) and 46 healthy subjects (median age, 12.8 y; median BMI-SDS, 0.34; median HbA 1c 5.4%) were recruited. Serum levels of CRP were significantly elevated (p ϭ 0.005) and retinol concentrations were significantly lower (p ϭ 0.02) in children and adolescents with type 1 diabetes compared with healthy subjects. Serum RBP4 and TTR showed no differences between the groups. Healthy children with CRP levels above 0.6 mg/L had significant lower levels of retinol (p ϭ 0.03). This was not observed in children with type 1 diabetes. The results suggest that, in contrast to healthy children, minor CRP elevation does not affect vitamin A transport complex in serum of children with type 1 diabetes. (Pediatr Res 62: 741-745, 2007)

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“…Water was added to the samples to adjust the aqueous/organic ratio to prevent the formation of an emulsion [18]. Five percent potassium hydroxide was added for saponification to remove significant matrix interference from the high level of lipids in the samples [18][19][20]. To minimize the loss of lutein and zeaxanthin, we chose a low-concentration alkali saponification and shortened the saponification duration.…”

Section: Optimzation Of Sample Preparation For Breast Milk Samplesmentioning

confidence: 99%

Determination of Carotenoids in Human Serum and Breast Milk Using High Performance Liquid Chromatography Coupled with a Diode Array Detector (HPLC-DAD)

2017

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High performance liquid chromatography (HPLC) coupled with a diode array detector (HPLC-DAD) for the identification and quantification of carotenoids, namely all-trans lutein, zeaxanthin, β-cryptoxanthin, α-carotene, and β-carotene, in biological samples such as human serum and breast milk, has been developed and validated. Good chromatography separation was achieved using a binary mobile phase system on a YMC C 30 column (150 × 2.1 mm, 3 µm) at 30 • C. Owing to the smaller column particle size and diameter of the column, the separation was achieved in 18 min, which is significantly reduced from the typical 30-40 min of other methods. The diode array detector (DAD) acquisition was set at a wavelength of 445 nm; 3D spectra ranging from wavelengths of 240-600 nm were also recorded. Peaks were identified by matching their retention time and spectra with those of standards. Quantification was achieved by internal standard calibration using echinenone as the internal standard. Good linearity was obtained for each compound (R 2 > 0.9999). The method quantification limits (MQLs) for serum and breast milk were 10 ng/mL and 5 ng/mL, in matrix, respectively. A spike recovery study and standard reference material (SRM) from the National Institute of Standards and Technology (NIST) 968e analysis has proven that the method has a high degree of accuracy, precision, and robustness. The stability study showed that the carotenoid standard and sample extracts could be stored in a chilled autosampler at 8 • C up to 48 h without being comprised, which provides guidance on re-test time frames. The freeze/thaw process was found to be detrimental to carotenoids, and should always be avoided. Most importantly, UV standardization of the stock standard is to be performed prior to each assay, and simply taking the values on Certificate of Analysis (CoA) for calculation of the standard concentration is not recommended.

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Improved Extraction Procedure for Carotenoids from Human Milk

Cited by 34 publications

References 9 publications

Low Plasma α-Tocopherol Concentrations and Adverse Clinical Outcomes in Diabetic Hemodialysis Patients

Low Plasma α-Tocopherol Concentrations and Adverse Clinical Outcomes in Diabetic Hemodialysis Patients

High-Normal C-Reactive Protein Levels Do Not Affect the Vitamin A Transport Complex in Serum of Children and Adolescents with Type 1 Diabetes

Determination of Carotenoids in Human Serum and Breast Milk Using High Performance Liquid Chromatography Coupled with a Diode Array Detector (HPLC-DAD)

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