Improved ethanol production from the slurry of pretreated brewers’ spent grain through different co-fermentation strategies

Rojas-Chamorro, José A.; Romero‐García, Juan Miguel; Cara, Cristóbal; Romero, Inmaculada; Castro, Eulógio

doi:10.1016/j.biortech.2019.122367

Cited by 32 publications

(21 citation statements)

References 45 publications

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“…Alternatively, the hydrolysis of BSG can be carried out through alkali, acid, hydrothermal, or enzymatic pre-treatments (Rojas-Chamorro et al 2020 ; Paz et al 2019 ). Ethanologenic microorganisms have been used to produce ethanol from these hydrolyzed BSG, including Saccharomyces cerevisiae and Zigosaccharomyces rouxii (Liguori et al 2015 ).…”

Section: Brewers’ Spent Grain In Industrial Biotechnologymentioning

confidence: 99%

The role of microorganisms on biotransformation of brewers’ spent grain

Bianco

Budroni

Zara

et al. 2020

Appl Microbiol Biotechnol

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Brewers’ spent grain (BSG) is the most abundant by-product of brewing. Due to its microbiological instability and high perishability, fresh BSG is currently disposed of as low-cost cattle feed. However, BSG is an appealing source of nutrients to obtain products with high added value through microbial-based transformation. As such, BSG could become a potential source of income for the brewery itself. While recent studies have covered the relevance of BSG chemical composition in detail, this review aims to underline the importance of microorganisms from the stabilization/contamination of fresh BSG to its biotechnological exploitation. Indeed, the evaluation of BSG-associated microorganisms, which include yeast, fungi, and bacteria, can allow their safe use and the best methods for their exploitation. This bibliographical examination is particularly focused on the role of microorganisms in BSG exploitation to (1) produce enzymes and metabolites of industrial interest, (2) supplement human and animal diets, and (3) improve soil fertility. Emerging safety issues in the use of BSG as a food and feed additive is also considered, particularly considering the presence of mycotoxins. Key points • Microorganisms are used to enhance brewers’ spent grain nutritional value. • Knowledge of brewers’ spent grain microbiota allows the reduction of health risks.

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Section: Brewers’ Spent Grain In Industrial Biotechnologymentioning

confidence: 99%

The role of microorganisms on biotransformation of brewers’ spent grain

Bianco

Budroni

Zara

et al. 2020

Appl Microbiol Biotechnol

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“…This yield could be further enhanced by microorganisms that co-utilise pentose and hexose sugars. Rojas-Chamorro et al [77] achieved an ethanol yield of 86% by E. coli SL100 on a BSG hydrolysate with high xylose content. However, they reached the maximum ethanol yield after 91 h of fermentation due to the slower utilisation of xylose [77].…”

Section: Bioethanol Productionmentioning

confidence: 99%

“…Rojas-Chamorro et al [77] achieved an ethanol yield of 86% by E. coli SL100 on a BSG hydrolysate with high xylose content. However, they reached the maximum ethanol yield after 91 h of fermentation due to the slower utilisation of xylose [77]. White et al [78] also investigated the use of xylose and arabinose on BSG hydrolysate by Pichia stiptitis and Kluyveromyces marxianus.…”

Section: Bioethanol Productionmentioning

confidence: 99%

Optimised Fractionation of Brewer’s Spent Grain for a Biorefinery Producing Sugars, Oligosaccharides, and Bioethanol

et al. 2021

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Brewer’s spent grain (BSG) is the main by-product of the beer brewing process. It has a huge potential as a feedstock for bio-based manufacturing processes to produce high-value bio-products, biofuels, and platform chemicals. For the valorisation of BSG in a biorefinery process, efficient fractionation and bio-conversion processes are required. The aim of our study was to develop a novel fractionation of BSG for the production of arabinose, arabino-xylooligomers, xylose, and bioethanol. A fractionation process including two-step acidic and enzymatic hydrolysis steps was investigated and optimised by a response surface methodology and a desirability function approach to fractionate the carbohydrate content of BSG. In the first acidic hydrolysis, high arabinose yield (76%) was achieved under the optimised conditions (90 °C, 1.85 w/w% sulphuric acid, 19.5 min) and an arabinose- and arabino-xylooligomer-rich supernatant was obtained. In the second acidic hydrolysis, the remaining xylan was solubilised (90% xylose yield) resulting in a xylose-rich hydrolysate. The last, enzymatic hydrolysis step resulted in a glucose-rich supernatant (46 g/L) under optimised conditions (15 w/w% solids loading, 0.04 g/g enzyme dosage). The glucose-rich fraction was successfully used for bioethanol production (72% ethanol yield by commercial baker’s yeast). The developed and optimised process offers an efficient way for the value-added utilisation of BSG. Based on the validated models, the amounts of the produced sugars, the composition of the sugar streams and solubilised oligo-saccharides are predictable and variable by changing the reaction conditions of the process.

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“…For determining the concentration of ethanol sample was centrifuged and supernatant was collected separately. And sample was analysed by GC [15].…”

Section: Scale Up Of Ssfmentioning

confidence: 99%

“…However, cost for using two different systems for separate enzyme production and the instability of genetically engineered strains is major problem being faced by the industry. Co-culture is a technique which has been used by researchers where combination of two microbes have been employed to carry out the stepwise conversion in a controlled way [15]. Simultaneous sacchari cation and fermentation has been explored by the researchers by employing various combinations of microorganisms and enzymes.…”

Section: Introductionmentioning

confidence: 99%

Production of bioethanol from wheat straw via optimization of co-culture conditions of Bacillus licheniformis and Saccharomyces cerevisiae

Sharma

Jha

Panwar

2021

Preprint

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Bioethanol production has been a challenge for the researchers to enhance the bioethanol yield. In this study, we are reporting an efficient novel method to produce bioethanol. The process comprises co-culture technique to produce bioethanol from wheat straw by co-culturing Bacillus licheniformis and Saccharomyces cerevisiae. Simultaneous saccharification and fermentation allows wheat straw hydrolysis by cellulase enzyme produced by Bacillus licheniformis and conversion of produced reducing sugar into ethanol by Saccharomyces cerevisiae. Pre-treatment of wheat straw and optimization of co-culturing parameters like, time, pH, substrate concentrations and nitrogen source concentrations gave a net yield of 5.67% v/v bioethanol. Scale up of optimised media to fermenter has resulted in a significant enhancement of bioethanol production to 18.64% (v/v).

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Improved ethanol production from the slurry of pretreated brewers’ spent grain through different co-fermentation strategies

Cited by 32 publications

References 45 publications

The role of microorganisms on biotransformation of brewers’ spent grain

The role of microorganisms on biotransformation of brewers’ spent grain

Optimised Fractionation of Brewer’s Spent Grain for a Biorefinery Producing Sugars, Oligosaccharides, and Bioethanol

Production of bioethanol from wheat straw via optimization of co-culture conditions of Bacillus licheniformis and Saccharomyces cerevisiae

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