Improved estimation of the incidence of Microdochium nivale in winter wheat stems in England and Wales, during 1992, by use of benomyl agar

Pettitt, Timothy; Parry, D. W.; Polley, Romina

doi:10.1016/s0953-7562(09)81279-3

Cited by 32 publications

(20 citation statements)

References 6 publications

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“…Attempts to determine the proportion of each species in plant samples by isolation into axenic culture may be confounded because, in the absence of selective media, the relative amounts of each pathogen may not be accurately measured. For example, F. culmorum tends to outcompete Microdochium nivale, which may result in an underestimation of the prevalence of the latter in mixed infections (Pettitt et al, 1993). Whatever the method, the isolation of pathogens from plant tissues can only reveal what may be grown out of the plant rather than what is within the plant.…”

Section: Introductionmentioning

confidence: 99%

Refinement of PCR‐detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum

Lees²,

et al. 1998

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Existing PCR-based assays for the detection of the cereal pathogen Fusarium avenaceum were found to cross-react with F. tricinctum. An investigation into the phenetic relationship between these two species using two different marker systems revealed a close relationship between them despite their being from separate taxonomic sections. Whilst RFLP differences in the ITS regions surrounding the nuclear 5·8 S rDNA were insufficient to discriminate between isolates of the two species, they were clearly differentiated by RAPD profiling. RAPD fragments from F. avenaceum isolates were screened for hybridization to F. tricinctum DNA on Southern blots. One of 12 selected RAPD fragments showed no hybridization to genomic DNA from F. tricinctum. This fragment was cloned and sequenced, and the sequence obtained was used to design PCR primers. The primers were found to be specific for F. avenaceum, with no cross-reactions obtained with F. tricinctum or any other wheat pathogen assayed. The primers were able to differentiate between the two species in infected plant material, in contrast to the earlier assays.

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Section: Introductionmentioning

confidence: 99%

Refinement of PCR‐detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum

Lees²,

et al. 1998

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“…Microdochium nivale (Fries) Samuels and Hallett (teleomorph Monographella nivalis (Schaffnit) Müller) is distributed worldwide and causes snow mould of cereals and turfgrasses (Cook, 1981), foot rot (Harris, 1986;Pettitt et al, 1993) and head blight diseases of cereals when high humidity and low temperature occur during anthesis (Cassini, 1981;Cook, 1981). It has been recorded as one of the dominant pathogen species identified from wheat samples in Western Europe (Duben and Fehrmann, 1979; Parry, 1990;Daamen et al, 1991;Maurin and Chénet, 1993), and resistance tests with artificial inoculation have been developed in order to assess levels of resistance of cereals to M nivale (Bruehl, 1982;Litschko et al, 1988;Miedaner et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

A comparison of biological, molecular and enzymatic markers to investigate variability within Microdochium nivale (Fries) Samuels and Hallett

Maurin¹,

Rezanoor

Lamkadmi³

et al. 1995

Agronomie

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“…In UK surveys, Microdochium nivale was the predominant Fusarium pathogen isolated from winter wheat seeds (Reeves and Wray 1994) and stem-bases (Locke et al 1987;Parry 1990;Pettitt et al 1993;Polley and Turner 1995). However, the source of M. nivale inoculum causing foot rot was not determined.…”

Section: Discussionmentioning

confidence: 99%

“…Surveys in the UK have shown M. nivale to be the predominant pathogen on stem-bases of winter wheat (e.g. Locke et al 1987;Pettitt et al 1993). It has been proposed, in diagrams of Fusarium disease cycles ) that non-lethal seedling blight may act as a source of inoculum for the subsequent development of foot rot and ear blight (Fig.…”

mentioning

confidence: 99%

The effect of a mixture of seed-borne Microdochium nivale var. majus and Microdochium nivale var. nivale infection on Fusarium seedling blight severity and subsequent stem colonisation and growth of winter wheat in pot experiments

Haigh

Jenkinson²,

Hare

2008

Eur J Plant Pathol

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Greenhouse experiments were conducted in order to determine the impact of seed-borne Microdochium nivale var. nivale and var. majus inoculum, and seed treatment with a carboxin+thiram mixture, on the development of seedling blight, and on subsequent stem colonisation and growth of winter wheat (cv. Cadenza). Experiments were conducted at temperatures favourable (3°C) and unfavourable (22°C) to M. nivale. Seed-borne inoculum resulted in seedling blight symptom development when plants were grown at 3°C, but not when plants were grown at 22°C. For seedlings grown at 3°C, plants arising from heavily blighted seedlings developed more severe symptoms of stem colonisation, when compared with those arising from seedlings from carboxin+thiram treated seeds. In addition, the vigour of such plants (assessed by determining the number of tillers and ears per plant, stem length, green leaf area, dry weight and yield) was also significantly lower than for plants arising from carboxin+thiram treated seeds. Microdochium nivale var. majus and var. nivale appeared to have little effect on plant vigour from seedlings grown at 22°C. This is the first recorded incidence of seedling blight affecting subsequent plant growth. Microdochium nivale var. majus and var. nivale stem colonisation increased from growth stage (GS) 40-49 to harvest in plants raised from seedlings grown at both temperatures. Microdochium nivale var. majus and var. nivale were isolated from the second node at GS 40-49 and the third node at harvest of plants from seedlings grown at 3°C. For plants from seedlings raised at 22°C, M. nivale var. majus and var. nivale were isolated from the first node at GS 40-49 and the second node at harvest. Carboxin+thiram seed treatment decreased the extent and severity of stem colonisation on plants from seedlings grown at 22°C.

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Improved estimation of the incidence of Microdochium nivale in winter wheat stems in England and Wales, during 1992, by use of benomyl agar

Cited by 32 publications

References 6 publications

Refinement of PCR‐detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum

Refinement of PCR‐detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum

A comparison of biological, molecular and enzymatic markers to investigate variability within Microdochium nivale (Fries) Samuels and Hallett

The effect of a mixture of seed-borne Microdochium nivale var. majus and Microdochium nivale var. nivale infection on Fusarium seedling blight severity and subsequent stem colonisation and growth of winter wheat in pot experiments

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