Improved estimation of biological nitrogen fixation of soybean cultivars (Glycine max L. Merril) using 15N natural abundance technique

Houngnandan, Pascal; Yemadje, R. G. H.; Oikeh, Sylvester O.; Djidohokpin, C. F.; Boeckx, Pascal; Cleemput, Oswald Van

doi:10.1007/s00374-008-0311-5

Cited by 29 publications

(15 citation statements)

References 32 publications

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“…The %Ndfa of the two cultivars is comparable to the upper (69%) and lower (36%) quartiles of a meta-analysis of N 2 fixation in soybean at ambient [CO 2 ] (Salvagiotti et al 2008). The difference in %Ndfa between cultivars, which implies the variation in N 2 fixation ability among genotypes, was also reported in other soybean N 2 fixation studies at ambient [CO 2 ] (Danso et al 1987;Houngnandan et al 2008;Matsunami et al 2009). Elevated [CO 2 ] increased %Ndfa for Zhonghuang 13 from 59% to 79% (p00.07, Fig.…”

Section: Nitrogen Fixationsupporting

confidence: 76%

Effect of elevated carbon dioxide on growth and nitrogen fixation of two soybean cultivars in northern China

et al. 2012

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The effect of elevated carbon dioxide (CO 2 ) concentration on symbiotic nitrogen fixation in soybean under open-air conditions has not been reported. Two soybean cultivars (Glycine max (L.) Merr. cv. Zhonghuang 13 and cv. Zhonghuang 35) were grown to maturity under ambient (415±16 μmol mol −1 ) and elevated (550±17 μmol mol −1 ) [CO 2 ] at the free-air carbon dioxide enrichment experimental facility in northern China. Elevated [CO 2 ] increased above-and below-ground biomass by 16-18% and 11-20%, respectively, but had no significant effect on the tissue C/N ratio at maturity. Elevated [CO 2 ] increased the percentage of N derived from the atmosphere (%Ndfa, estimated by natural abundance) from 59% to 79% for Zhonghuang 13, and the amount of N fixed from 166 to 275 kg N ha −1 , but had no significant effect on either parameter for Zhonghuang 35. These results suggest that variation in N 2 fixation ability in response to elevated [CO 2 ] should be used as key trait for selecting cultivars for future climate with respect to meeting the higher N demand driven by a carbon-rich atmosphere.

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Section: Nitrogen Fixationsupporting

confidence: 76%

Effect of elevated carbon dioxide on growth and nitrogen fixation of two soybean cultivars in northern China

et al. 2012

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“…Subsequently, the NA-weed method resulted in higher observed N fixed values and greater method precision than the NA-wheat method, particularly in 2010 when mean d 15 N differences between broadleaf weeds and both legumes consistently exceeded 2%. Differences in d 15 N values among reference species grown in close proximity have been observed by a number of investigators (Bremer et al 1993;Hauggaard-Nielsen et al 2010;Houngnandan et al 2008;Pate et al 1994) and attributed to different isotopic discrimination among species, dissimilar rates of plant N uptake throughout the growing season, and different rooting patterns. These are all possible reasons for the observed differences in N fixation by the NA-wheat and NAweed methods in this study and further emphasize the importance of using multiple reference plants when utilizing the NA method (Unkovich et al 2008).…”

Section: Comparison Of N Fixation Methodsmentioning

confidence: 93%

Nitrogen fixation by pea and lentil green manures in a semi-arid agroecoregion: effect of planting and termination timing

McCauley

Jones

Miller

et al. 2012

Nutr Cycl Agroecosyst

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Crop-fallow systems dominate many semi-arid agricultural regions despite fallow's negative effects on soil and water quality. Annual legumes grown as a fallow-replacement crop, and terminated prior to maturity, can reduce these negative effects without substantially decreasing plant available water for the subsequent crop. Interest in growing legume green manures (LGMs) in synthetically-fertilized systems is increasing in the northern Great Plains of North America, partly due to the N-fixing capabilities of legumes; however, little is known about the effects of planting and termination time on N fixation amounts in the region. A 2-year field study was initiated in southwest Montana to determine the effects of planting time (spring or summer) and termination time (e.g. flower or pod) on the amount of N fixed by field pea (Pisum sativum cv. Arvika) and lentil (Lens culinaris cv. Richlea). Two methods, 15 N natural abundance and N difference, were used to quantify N fixation, with wheat or in-crop weeds as reference plants. In 2009, N fixed by spring-planted lentil was higher by pod than flower (P = 0.03). Termination time did not affect the amount of N fixed by spring-planted pea, despite more biomass by pod than flower. In 2010, both spring-planted crops fixed more N by pod than flower (P \ 0.01) and more N was fixed by spring-planted than summer-planted crops (P \ 0.01). These results should prove useful to growers interested in selecting management practices that optimize N fixation of LGMs.

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“…As reported by Houngnandan et al (2008) in the last decades, isotope ratio mass spectrometry has been used to detect small variations in 15 N abundance in soil and plant materials. These changes can be used for assessing N 2 fixation under field conditions without the additional costs of 15 N-enriched fertilizer.…”

Section: Introductionmentioning

confidence: 99%

B value and isotopic fractionation in N2 fixation by chickpea (Cicer arietinum L.) and faba bean (Vicia faba L.)

López‐Bellido

Redondo

et al. 2010

Plant Soil

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A prerequisite for the calculation of the proportion of a legume's nitrogen derived from the atmosphere (%Ndfa) using the 15 N natural abundance technique (NA) is the determination of the B value (δ 15 N value for a legume when completely dependent on N 2 fixation for growth). With this objective, Kabuli-type chickpea seeds were inoculated with Mesorhizobium ciceri, strain ISC-6, and an indigenous strain mixture (ISM) from the Guadalquivir Valley (southern Spain); and mayor-equina-type faba bean seeds were inoculated with Rhizobium leguminosarum strain ISL-19. Both legumes were grown in perlite with an N-free nutrient solution and in a growth chamber; uninoculated plants were also grown under the same conditions. Samples of the inoculated plants were collected at full flowering and maturity, analyzing total N and 15 N content of the nodules, root, shoot and seeds. The seeds sown and uninoculated plants at wilting were also analyzed. The uninoculated chickpea and faba bean plants showed strong 15 N discrimination in the remobilization of reserve proteins from the cotyledons to the shoot. Only 52% of the 15 N from the chickpea seed and 46% from the faba bean seed were translocated to the shoot. The B whole plant value for both inoculated species (−1.44‰ in chickpea and −1.33‰ in faba bean) demonstrated the existence of a significant isotopic discrimination of N in the fixation of atmospheric N 2 . The B shoot value did not show significant differences between full flowering and seed maturity for either species, which allowed field sampling to be performed for the calculation of % Ndfa between both growth stages. Each species presented different 15 N discrimination for the different parts analyzed. In chickpea, strong discrimination was observed between the nodules (+3.11‰) and the rest of the plant (−1.67‰); this discrimination was less pronounced in faba bean and always with negative values between the underground parts (nodules and root, with a mean value of −0.75‰) and the shoot (−1.71‰). The B shoot value obtained was −1.80‰ in chickpea and −1.71‰ in faba bean. However, given the importance of the amount of seed N in chickpea and faba bean, seed 15 N contribution should be considered in the calculation of the B shoot value using Plant Soil (2010) 337:425-434 a mass balance model which considers 50% of this amount. In this case, the adjusted B shoot values would increase to −2.05‰ and −1.89‰ in chickpea and faba bean, respectively.

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Improved estimation of biological nitrogen fixation of soybean cultivars (Glycine max L. Merril) using 15N natural abundance technique

Cited by 29 publications

References 32 publications

Effect of elevated carbon dioxide on growth and nitrogen fixation of two soybean cultivars in northern China

Effect of elevated carbon dioxide on growth and nitrogen fixation of two soybean cultivars in northern China

Nitrogen fixation by pea and lentil green manures in a semi-arid agroecoregion: effect of planting and termination timing

B value and isotopic fractionation in N2 fixation by chickpea (Cicer arietinum L.) and faba bean (Vicia faba L.)

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