Improved endothelial cell adhesion and proliferation on patterned titanium surfaces with rationally designed, micrometer to nanometer features

J, Lu; Rao, Masaru P.; MacDonald, N.C.; Khang, Dongwoo; Webster, Thomas J.

doi:10.1016/j.actbio.2007.07.008

Cited by 251 publications

(209 citation statements)

References 37 publications

Supporting

Mentioning

197

Contrasting

Unclassified

Order By: Relevance

“…Materials with nano-scale features have shown promise in enhancing various applications, including fuel cells 1 , sensors 2,3 , and biomedical devices 4,5 . A relatively new material is nanoporous gold (np-Au), which is produced by a nano-scale self-assembly process.…”

Section: Introductionmentioning

confidence: 99%

Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies

Daggumati

Kurtuluş

Dimlioglu

et al. 2013

JoVE

View full text Add to dashboard Cite

Nanostructured materials with feature sizes in tens of nanometers have enhanced the performance of several technologies, including fuel cells, biosensors, biomedical device coatings, and drug delivery tools. Nanoporous gold (np-Au), produced by a nano-scale self-assembly process, is a relatively new material that exhibits large effective surface area, high electrical conductivity, and catalytic activity. These properties have made np-Au an attractive material to scientific community. Most studies on np-Au employ macro-scale specimens and focus on fundamental science of the material and its catalytic and sensor applications. The macro-scale specimens limit np-Au's potential in miniaturized systems, including biomedical devices. In order to address these issues, we initially describe two different methods to micropattern np-Au thin films on rigid substrates. The first method employs manually-produced stencil masks for creating millimeter-scale np-Au patterns, while the second method uses lift-off photolithography to pattern sub-millimeter-scale patterns. As the np-Au thin films are obtained by sputter-deposition process, they are compatible with conventional microfabrication techniques, thereby amenable to facile integration into microsystems. These systems include electrically-addressable biosensor platforms that benefit from high effective surface area, electrical conductivity, and gold-thiol-based surface bioconjugation. We describe cell culture, immunostaining, and image processing techniques to quantify np-Au's interaction with mammalian cells, which is an important performance parameter for some biosensors. We expect that the techniques illustrated here will assist the integration of np-Au in platforms at various length-scales and in numerous applications, including biosensors, energy storage systems, and catalysts. Video LinkThe video component of this article can be found at

show abstract

Section: Introductionmentioning

confidence: 99%

Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies

Daggumati

Kurtuluş

Dimlioglu

et al. 2013

JoVE

View full text Add to dashboard Cite

show abstract

“…The method described here presents a straightforward and time-efficient method to produce cell aligning features and to also cellularize with relative precision so that etching and bioprinting can both be performed under an hour. Other methods of producing aligning channels and grooves include deep reactive ion etching [26] , electron beam lithography [12] , direct laser writing [27] , femtosecond laser [22] , photolithography [28] , plasma dry etching [29] etc. (as reviewed by Li and colleagues [7] ).…”

Section: Discussionmentioning

confidence: 99%

A novel 3D printing method for cell alignment and differentiation

Bhuthalingam¹,

Lim²,

Irvine³

et al. 2015

ijb

View full text Add to dashboard Cite

Abstract:The application of bioprinting allows precision deposition of biological materials for bioengineering applications. Here we propose a 2 stage methodology for bioprinting using a back pressure-driven, automated robotic dispensing system. This apparatus can prepare topographic guidance features for cell orientation and then bioprint cells directly onto them. Topographic guidance features generate cues that influence adhered cell morphology and phenotype. The robotic dispensing system was modified to include a sharpened stylus that etched on a polystyrene surface. The same computer-aided design (CAD) software was used for both precision control of etching and bioink deposition. Various etched groove patterns such as linear, concentric circles, and sinusoidal wave patterns were possible. Fibroblasts and mesenchymal stem cells (MSC) were able to sense the grooves, as shown by their elongation and orientation in the direction of the features. The orientated MSCs displayed indications of lineage commitment as detected by fluorescence-activated cell sorting (FACS) analysis. A 2% gelatin bioink was then used to dispense cells onto the etched features using identical, programmed co-ordinates. The bioink allows the cells to contact sense the pattern while containing their deposition within the printed pattern.

show abstract

“…Проведено изучение ад-гезии, пролиферации и морфологии ЕС на узор-чатой титановой поверхности с параллельными дорожками шириной от 750 нм до 100 мкм и та-ким же расстоянием между ними [62]. На этих поверхностях ECs адгезировались в углублениях дорожек и имели правильную вытянутую форму и ориентацию.…”

Section: повышение биосовместимости металличе-ских поверхностей с помunclassified

Surface Modification of Bare-Metal Stents for Preventing Restenosis (Part 2)

Лотков¹,

Матвеева²,

Антонова³

et al. 2017

Kompleks. probl. serdečno-sosud. zabol.

View full text Add to dashboard Cite

Резюме. Вторая часть обзора посвящена вариантам модификации металлических стентов для ускоре-ния эндотелизации in situ. Представлены разработки. направленные на захват эндотелиальных прогени-торных клеток из кровотока с помощью специфических антител. Описаны возможности улучшения адгезии и интеграции эндотелиальных клеток на поверхности за счет формирования сайтов клеточного распозна-вания и имитация структур внеклеточного матрикса. Проанализированы различные способы физической и химической модификации, способствующие созданию условий для скорейшего формирования функцио-нально состоятельного эндотелиального слоя на искусственных поверхностях. Определен круг проблем и ограничений в использовании каждого из методов.Ключевые слова: металлические стенты, рестеноз, модификация поверхности, эндотелизация. Here we review the modifications of the metal stents to enhance in situ endothelialization, particularly antibody-mediated adhesion of endothelial progenitor cells. We focus on the strategies employing cell adhesion molecules and extracellular matrix proteins. We also encompass other physical and chemical approaches for surface biofunctionalization with their advantages and shortcomings. SURFACE MODIFICATION OF BARE-METAL STENTS FOR PREVENTING RESTENOSISKeywords: bare-metal stents, restenosis, surface modification, endothelialization НИИ КПССЗ имеет большой опыт в разработке изделий для сердечно-сосудистой хирургии, в частности биопротезов кла-панов сердца и сосудов. С 2010 года под руководством академика РАН л. С. Барбараша начаты исследования по созданию био-резорбируемых сосудистых протезов малого диаметра. Основная цель -создание полимерных конструкций с бионаправленным действием, способных заменить отдельные структуры живого организма, в частности сердечно-сосудистой системы. Научная но-визна поставленной задачи заключается в использовании нового подхода создания органа непосредственно в организме пациента за счет биофункциональности и биорезорбируемости полимерных конструкций. доказана долгосрочная проходимость полимерных сосудистых графтов на основе поликапролактона и композиции полигидроксибутирата/валерата и поликапролактона. В экспери-ментах in vitro доказано, что ростовые факторы, инкорпорируемые в состав биодеградируемых графтов, сохраняют свою био-логическую активность. В долгосрочных экспериментах in vivo доказано, что сосудистый эндотелиальный фактор роста ускоряет эндотелизацию и улучшает проходимость биодеградируемых полимерных сосудистых графтов.Ключевые слова: биодеградируемые полимеры, сосудистый графт, ростовые факторы. DEVELOPMENT OF TISSUE ENGINEERED SMALL DIAMETER VASCULAR GRAFT FOR THE CARDIOVASCULAR SURGERY NEEDS L. V. ANTONOVA, YU. A. KUDRYAVTSEVA Federal State Budgetary Scientific Institution Research Institute for Complex Issues of Cardiovascular Diseases. Kemerovo, RussiaRICICd has large experience in the development of products for cardiovascular surgery, in particular, the bioprosthesis heart valves and blood vessels. Since 2010, under the leadership of the academician of RAS L. S. Bar...

show abstract

Improved endothelial cell adhesion and proliferation on patterned titanium surfaces with rationally designed, micrometer to nanometer features

Cited by 251 publications

References 37 publications

Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies

Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies

A novel 3D printing method for cell alignment and differentiation

Surface Modification of Bare-Metal Stents for Preventing Restenosis (Part 2)

Contact Info

Product

Resources

About