Improved efficiency of doubled haploid generation in hexaploid triticale by in vitro chromosome doubling

Würschum, Tobias; Tucker, Matthew R.; Reif, Jochen C.; Maurer, Hans Peter

doi:10.1186/1471-2229-12-109

Cited by 47 publications

(30 citation statements)

References 19 publications

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“…Acquiring embryogenic competence requires the application of some type of external cue(s) leading to a stress(s) that preventing microspores from continuing their normal mode of gametophytic development and inducing sporophytic differentiation. In the genus Brassica, microspores are often induced by heat shock (Prem et al 2005), anti-microtubular (Würschum et al 2012), and mutagenic agents (Ahmadi et al 2012), stress hormones (Ahmadi et al 2014a) or polyamines (Ahmadi et al 2014b). Upon stress treatment and embryogenesis induction, many biochemical and morphological changes occur which is closely related to alterations in gene expression pattern (Tsuwamoto et al 2007;Muñoz-Amatriaín et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

et al. 2015

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The BnSERK1 and BnSERK2 are involved in the process of microspore embryogenesis induction, development, and plantlet regeneration. Little is known about regulatory role of somatic embryogenesis-related kinase (SERK) genes family in the induction of microspore embryogenesis, development and plant regeneration. In this study, the expression of two SERK genes (SERK1 and SERK2) was assessed during the microspore embryogenesis and plantlet regeneration in Brassica napus L. The BnSERK1 was severely up-regulated 1-5 days following microspore culture and its expression drastically decreased in the globular-heart and also torpedo staged microspore-derived embryos (MDEs). In addition, high levels of BnSERK1 transcript were detected in the MDE maturation phase and in the roots and shoots of the regenerated plantlets which indicates a broader role(s) of BnSERK1 in the organ formation, rather than being specific to the embryogenesis. Results of partial sequencing indicated that the BnSERK1 shares a conserved serine-threonine kinase catalytic domain and exhibited 95 % similarity with AtSERK1, CsSERK1, BrSERK1, NaSERK1, and NbSERK1. A steady increase in the expression of BnSERK2 was observed during the MDE initiation and development so that, the highest expression was noted in the MDE maturation phase i.e., late cotyledonary MDEs. Our results also indicated low amounts of BnSERK2 transcript at the onset of rhyzogenesis but significantly higher expression in the developing roots. In contrast, the BnSERK2 strongly up-regulated during the both initially and developed shoots. The BnSERK2 shares highly conserved LRR-RLK domain when compared with different species tested so that, high homology (100 %) was noticed with BrSERK2. Based on our findings, MDE formation and plantlet regeneration seem to be correlated with both BnSERK1 and BnSERK2 expression.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

et al. 2015

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“…and rye (Secale cereale L.) developed to combine grain quality, productivity, and disease resistance of wheat with rye's excellent environmental adaptability and tolerance. It is amenable to androgenesis (Immonen and Robinson 2000;Oleszczuk et al 2004;Ponitka and Slusarkiewicz-Jarzina 2007;Würschum et al 2012) and registered cultivars have been created via the doubled haploid approach (Dr. Z. Banaszak-Plant Breeding Danko Ltd Poland, Dr. H. Wos-Plant Breeding Strzelce Ltd Poland, pers. comm.…”

Section: Introductionmentioning

confidence: 99%

The origin of clones among androgenic regenerants of hexaploid triticale

2014

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Doubled haploids (DH) have become a standard tool in breeding and genetic studies of many crops and in most cases androgenesis is the only available route of their production. It has been recently observed that some populations of DH lines obtained via androgenesis contain high proportions of clones. This seriously reduces the efficiency of breeding and may jeopardize genetic studies. This study was designed to determine at which stage of androgenesis these clones are created, using samples set aside during routine production of DH lines in breeding of hexaploid triticale. The fate of each androgenic structure was carefully followed through the entire regeneration process, and all obtained plants were genotyped using DNA markers. Overall, 189 plants were regenerated forming 33 families, each originating from a single original androgenic structure (callus, polyembryos). In ca. 80 % of cases all members of a family were genetically identical. However, in about 20 % of cases the families of regenerants were genetically heterogeneous, showing that not all androgenic structures originate from single microspores.The evidence shown here demonstrates that retention of single plants from each original structure guarantees the production of only unique genotypes but it reduces the total output of plants. If maximum output is desired, multiple regenerants from single callus can be retained but must be genotyped using at least 10 polymorphic markers to identify clones.

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“…In vitro application of colchicine is an alternative for doubled haploid production using microspore culture. Using this method of colchicine application, Würschum et al (2012) showed a pronounce increase in the proportion of doubled haploid triticale plants, thus a promising alternative to the in vivo approach. The antimicrotubule herbicides such as amiprophos methyl, oryzalin, and pronamide are effective in doubling chromosome sets of maize; being less toxic than that of colchicine (Häntzschel and Weber, 2010).…”

Section: Conventional Methods To Induce Doubled Haploidsmentioning

confidence: 96%

Haploids: Constraints and opportunities in plant breeding

Dwivedi

Britt

Tripathi

et al. 2015

Biotechnology Advances

226

134

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The discovery of haploids in higher plants led to the use of doubled haploid (DH) technology in plant breeding. This article provides the state of the art on DH technology including the induction and identification of haploids, what factors influence haploid induction, molecular basis of microspore embryogenesis, the genetics underpinnings of haploid induction and its use in plant breeding, particularly to fix traits and unlock genetic variation. Both in vitro and in vivo methods have been used to induce haploids that are thereafter chromosome doubled to produce DH. Various heritable factors contribute to the successful induction of haploids, whose genetics is that of a quantitative trait. Genomic regions associated with in vitro and in vivo DH production were noted in various crops with the aid of DNA markers. It seems that F2 plants are the most suitable for the induction of DH lines than F1 plants. Identifying putative haploids is a key issue in haploid breeding. DH technology in Brassicas and cereals, such as barley, maize, rice, rye and wheat, has been improved and used routinely in cultivar development, while in other food staples such as pulses and root crops the technology has not reached to the stage leading to its application in plant breeding. The centromere-mediated haploid induction system has been used in Arabidopsis, but not yet in crops. Most food staples are derived from genomic resources-rich crops, including those with sequenced reference genomes. The integration of genomic resources with DH technology provides new opportunities for the improving selection methods, maximizing selection gains and accelerate cultivar development. Marker-aided breeding and DH technology have been used to improve host plant resistance in barley, rice, and wheat. Multinational seed companies are using DH technology in large-scale production of inbred lines for further development of hybrid cultivars, particularly in maize. The public sector provides support to national programs or small-medium private seed for the exploitation of DH technology in plant breeding.

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Improved efficiency of doubled haploid generation in hexaploid triticale by in vitro chromosome doubling

Cited by 47 publications

References 19 publications

Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

Molecular characterization and expression analysis of SERK1 and SERK2 in Brassica napus L.: implication for microspore embryogenesis and plant regeneration

The origin of clones among androgenic regenerants of hexaploid triticale

Haploids: Constraints and opportunities in plant breeding

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