Improved effectiveness and efficiency in measuring soil enzymes as universal soil quality indicators using microplate fluorimetry

Trap, Jean; Riah, Wassila; Akpa‐Vinceslas, Marthe; Bailleul, Caroline; Laval, Karine; Trinsoutrot‐Gattin, Isabelle

doi:10.1016/j.soilbio.2011.10.010

Cited by 30 publications

(17 citation statements)

References 8 publications

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“…For example, Trap et al. () reported that the values of soil enzyme activity obtained by the spectrophotometric method were around eight times higher than those revealed by the fluorimetric method, and Deng et al. () found that results from these two methods were comparable.…”

Section: Methodsmentioning

confidence: 99%

“…Studies have shown that these two methods can yield inconsistent results. For example, Trap et al (2012) reported that the values of soil enzyme activity obtained by the spectrophotometric method were around eight times higher than those revealed by the fluorimetric method, and Deng et al (2013) found that results from these two methods were comparable. In our case, 31 pairs of soil enzyme activities used the microplate fluorimetric method, and 170 used the spectrophotometric method (Appendix S1: Table S1).…”

Section: Data Collectionmentioning

confidence: 99%

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Enhanced activity of soil nutrient‐releasing enzymes after plant invasion: a meta‐analysis

Zhou

Staver

2019

Ecology

107

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Plant invasion can significantly alter soil nutrient cycling of ecosystems. How these changes are linked to soil enzyme activities is still unknown, however, even though these are proximate agents of organic matter decomposition and nutrient release. We performed a meta‐analysis of 60 case studies examining responses of 10 unique soil enzymes to plant invasion, and tested whether invaded soils differed in their enzyme activities from uninvaded soils. We also examined whether increases in soil nutrient‐releasing enzyme activity were paralleled by enhanced soil nutrient availability after plant invasion. Overall, we found that plant invasion had significant impacts on the activities of seven types of soil enzymes. Plant invasion had inconsistent impacts on C‐decomposing enzymes, but invaded sites had significantly higher activities of soil enzymes related to N‐ and P‐release than noninvaded sites. Increases in nutrient‐releasing enzyme activity after plant invasion ranged from +23% to +69%, which potentially results in a linear increase of soil nutrient availability in response to enhanced enzyme activities. Invaded soils also had higher nutrient stocks and soil microbial biomass than uninvaded soils. Our results suggest that enhanced activity of soil nutrient‐releasing enzymes after plant invasion may accelerate nutrient cycling, potentially creating a nutrient‐rich soil environment that benefits invaders and promotes their persistence, as invasive plants often appear to be more resource‐demanding and competitive than native species.

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Section: Methodsmentioning

confidence: 99%

Section: Data Collectionmentioning

confidence: 99%

Enhanced activity of soil nutrient‐releasing enzymes after plant invasion: a meta‐analysis

Zhou

Staver

2019

Ecology

107

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“…The potential activities of three C‐acquiring enzymes (β‐1,4‐glucosidase, βG, EC 3.2.1.21; β‐D‐1,4‐cellobiosidase, CBH, EC 3.2.1.91; β‐1,4‐xylosidase, βX, EC 3.2.1.37), two N‐acquiring enzymes (β‐N‐acetyl glucosaminidase, NAG, EC 3.1.6.1; leucine aminopeptidase, LAP, EC 3.4.11.1), and one P‐acquiring enzyme (alkaline phosphatase, AP, EC 3.1.3.2) in the soil samples were measured following a modified fluorescence method (German et al, ; Trap et al, ). In brief, 1 g of fresh soil was homogenized in 125‐ml 2‐morpholin‐4‐ylethanesulfonic acid buffer (pH = 6.5) or 2‐amino‐2‐(hydroxymethyl) propane‐1,3‐diol (Tris) buffer (pH = 7.5).…”

Section: Methodsmentioning

confidence: 99%

“…The potential activities of three C-acquiring enzymes (β- (German et al, 2011;Trap et al, 2012). In brief, 1 g of fresh soil was homogenized in 125-ml 2-morpholin-4-ylethanesulfonic acid buffer (pH = 6.5) or 2-amino-2-(hydroxymethyl) propane-1,3-diol (Tris) buffer (pH = 7.5).…”

Section: Enzyme Assaysmentioning

confidence: 99%

Soil enzyme activity and stoichiometry along a gradient of vegetation restoration at the Karst Critical Zone Observatory in Southwest China

et al. 2019

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The “Grain for Green Programme” was implemented in the 1990s as a solution to the extreme degradation of karst landscapes that cover one‐third of China, largely caused by decades of poorly managed intensive agriculture. The recovery of soil functions is key to the success of ecosystem regeneration of abandoned croplands where the carbon (C) and nutrient cycles have been severely perturbed by cultivation. However, an ecological ‘tipping point’ beyond which soil functions are unrecoverable in manageable timescales may have been passed in the fragile, subtropical karst ecosystem. The aim of this study was to use the activity of key enzymes for C, nitrogen (N), and phosphorus (P) acquisition in the soil as a proxy for the biological response to vegetation restoration after agricultural abandonment in a severely degraded karst catchment at the Karst Critical Zone Observatory in Guizhou Province. In 2016, a space‐for‐time approach was used to establish a chronosequence of vegetation recovery: sloping cropland < recently abandoned sloping cropland < shrubland < secondary (regenerated) forest < primary (natural) forest. Soils were sampled from the surface to the bedrock (up to 80‐cm depth) in each recovery phase. The activity of all enzymes in the top 0 to 30‐cm depth increased after abandonment and was positively correlated with soil nutrient and water contents. Nitrogen deficiencies were indicated by the reduced ratios of C‐ relative to N‐hydrolase activity and the increased ratios of N‐ relative to P‐hydrolase activity in the abandoned croplands and shrublands. Phosphorus deficiencies were indicated by the reduced ratios of N‐ relative to P‐hydrolase activity and C‐ relative to P‐hydrolase activity in the soils of the shrubland and secondary forest compared with the primary forest. Our results revealed that near‐to‐natural biological soil function was recoverable as vegetation naturally restored and suggested that the rate of recovery may be accelerated by managed nutrient amendments during the early stages after abandonment. This new information may help to inform the managed regeneration of degraded agricultural land in nutrient‐poor, subtropical environments.

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“…Soil enzymes mainly belong to hydrolases, oxidoreductases, transferases and lyases (Tabatabai and Dick 2002). Hydrolases are the most commonly measured class of enzymes in soils (Burns et al 2013;Nannipieri et al 2002, Trap et al 2012Riah et al 2014). Enzymes respond to soil management changes before changes in other soil parameters are detectable.…”

Section: Introductionmentioning

confidence: 99%

Inter-Laboratory Validation Of An ISO Test Method For Measuring Enzyme Activities In Soil Samples Using Colorimetric Substrates

Cheviron

Grondin

Marrauld

et al. 2021

Preprint

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The evaluation of soil quality requires the use of robust methods to assess biologically based-indicators. Among them, enzyme activities are used for several decades, but there is a clear need to update their measurement methods for routine use, in combining feasibility, accuracy and reliability. To this end, the platform Biochem-Env optimised a miniaturised method to measure enzyme activities in soils using colorimetric substrates in micro-well plates. The standardization of the method was carried out within the framework of ISO/TC 190/SC 4/WG 4 "Soil quality – Biological methods” workgroup, recommending an inter-laboratory evaluation for the publication of a full ISO standard.That evaluation, managed by the platform, was based on the measurement, in six soils of contrasted physicochemical properties, of the ten soil enzyme activities described in the standard. Eight laboratories were involved in the validation study. Only 2.7% of outliers were identified from the analyses of the whole dataset. The repeatability and reproducibility of the method were determined by computing, respectively, the intra-laboratory (CVr,) and inter-laboratory (CVR) coefficients of variation for each soil and enzyme. The mean CVr ranged from 4.5% (Phosphatase) to 9.9% (αGlucosidase), illustrating a reduced variability of enzyme activities within laboratories. The mean CVR ranged from 13.8% (Alkaline Phosphatase) to 30.9% (Phosphatase).Nevertheless, the method was repeatable, reproducible and sensitive. It also proved to be applicable for measuring enzyme activities in different types of soils. These results have been found successful by ISO/TC 190/SC4 and resulted in the publication of ISO 20130:2018 standard.

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Improved effectiveness and efficiency in measuring soil enzymes as universal soil quality indicators using microplate fluorimetry

Cited by 30 publications

References 8 publications

Enhanced activity of soil nutrient‐releasing enzymes after plant invasion: a meta‐analysis

Enhanced activity of soil nutrient‐releasing enzymes after plant invasion: a meta‐analysis

Soil enzyme activity and stoichiometry along a gradient of vegetation restoration at the Karst Critical Zone Observatory in Southwest China

Inter-Laboratory Validation Of An ISO Test Method For Measuring Enzyme Activities In Soil Samples Using Colorimetric Substrates

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